The purpose of this paper is to share my first deployment experience as the first sergeant. Serving in a war zone is an experience you will never forget. As a result of my first deployment to Afghanistan as a first sergeant‚ I practiced a new perspective on life‚ instilling trust in my Soldiers and NCOs to continue the mission. Perspective that I developed from the beginning with the pre-deployment preparation at the National Training Center (NTC) through the actual deployment to Afghanistan and
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ADMS 101 0 EXPLORING THE FUNCTIONS OF BUSINESS TODAY Eytan Lasry‚ Atkinson 232‚ 416.736.2100 eytan@yorku.ca x. 22878‚ Agenda • About me • About the course • Intro to critical thinking About me About the a.k.a. Syllabus Course outline Course • Survey of the functions of business description – Accounting‚ Finance‚ Strategy‚ OB/HR‚ Operations… • Critical discourse about: – Management & sub-disciplines – Current & future business challenges • Course readings – Pair “classic” management
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microscopic assay and capillary assay using chemoeffectors like malate‚ glucose and streptomycin with E. carotovora as test organism. Results from the migration experiment showed P. aeruginosa‚ a polarly flagellated organism‚ swims faster than E. carotovora which is a peritrichously flagellated organism. In demonstration of motility and chemotaxis using microscopic assay‚ movement towards malate and away from streptomycin was clearly observed. Colony counts from using the capillary assay showed the
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and can be used directly without dilution. But sample A2 needed to be diluted 1 + 2 with distilled water before use i.e. I ml of A2 was delivered into a clean LP4 tube by glass pipette followed by 2 ml of water. This diluted sample was used for the assay. Sample D was also prepared in the same way but without dilution. Duplicate tubes were prepared from 200 micro litres of all samples A to D.Standards were prepared from the
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methylene bis-acrylamide (a cross linking agent) to acrylamide. The immobilized enzyme was then tested via spectrophotometric assay at 510nm for kinematic activity and stability relative to its free enzyme counterpart. The enzymatic reaction was induced and analyzed via the addition of hydrogen peroxide (H2O2)‚ phenol‚ and 4-aminoantipyrine. To test stability a spectrophotometric assay (again at 510 nm) was conducted after both immobilized and free enzymes were heated at 70˚C for four minutes. The results
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Adding Enzymes to Improve Beef Tenderness By Chris R. Calkins‚ Ph.D. and Gary Sullivan‚ University of Nebraska Importance of Beef Tenderness Beef palatability is affected by many factors and tenderness is cited as one of the most important. Consumers are willing to pay a premium for a guaranteed tender product with the potential to increase the value of the middle meats over $60 per carcass (Miller et al.‚ 2001). Consequently‚ the meat industry is in a continual search for methods to improve the
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One unit of CAT activity was defined as an absorbance change of 0.01 as units/min. Reduced glutathione assay (GSH) Reduced glutathione was estimated by the method of Jollow et al. [15]. 1.0 ml sample of 10% homogenate was precipitated with 1.0 ml of (4%) sulfosalicylic acid. The samples were kept at 4ºC for 1 hr and then centrifuged at 1200 × g for 20 min at 4ºC. The total volume of 3.0 ml assay mixture composed of 0.1 ml filtered aliquot‚ 2.7 ml phosphate buffer (0.1 M‚ pH 7.4) and 0.2 ml DTNB (5
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2- What is the application of isozymes in clinical diagnosis? Give the names of some enzymes used in diagnostic kits. Testing the serum content is a very useful tool in the clinical diagnosis in general. Normally the enzymes are present inside the cells and the enzyme activity in the serum is very low. As the tissue gets damaged‚ due to a disease or infection‚ enzymes can be released from the damaged cells into the serum. As enzymes are biological catalysts‚ the detection of the presence and the
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BIOCHEMISTRY 304 Enzyme Kinetic Sample Problems #1 September 2004 1 Given the reaction k1 kp E + S ES E + P k-1 where k1 = 1 x 107 M-1 sec-1 k-1 = 1 x 102 sec-1‚ and kp = 3 x 102 sec-1 a) Calculate Ks b) Calculate Km (a) k-1 1 x 102 sec-1 Ks = k1 = 1 x 107 M-1 sec-1 = 1 x 10-5 M (b) k-1 + kp (1 x 102 sec-1) + (3 x 102 sec-1)
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Running Head: JUSTIFICATION REPORT Benefits of a Keurig Coffee Maker Eric XXXX Strayer University Online English 315 Edward F. Palm‚ Ph.D November 12‚ 2011 November 12‚ 2011 Eric XXXX XXXXXXXXXXXXXX. XXXXXXXXXX XXXXXXXXXXX XXXXXXXX November 12‚ 2011 Mr. Mark Smith‚ COO Flight Evolution Concepts P.O. Drawer Z Ft Monroe‚ VA 23604 Dear Mr. Smith: Enclosed is a copy of my justification report regarding the funding and replacement of the current office coffee maker
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