Invertase is a type of enzyme‚ a natural catalytic agent for biochemical reactions‚ can be obtained in Baker’s Yeast. Determination of the effect of pH on invertase activity is the primary objective of the experiment. Dinitrosalicyclic acid (DNS) Assay method is utilized to monitor the enzymatic activity of invertase. Invertase was subjected to different pH (3.87‚ 4.0‚ 5.5‚ 7.3 and 10.55) of buffer solution and was observed under 540 nm absorbance using spectrophotometer. After observation and analysis
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COMMON TECHNICAL DOCUMENT CEFTEN (CEFTIBUTEN CAPSULES 400 MG) MODULE 3 QUALITY 3.1 MODULE 3 TABLE OF CONTENT Sr. No. | Contents | Page No. | 3.1 | MODULE 3 TABLE OF CONTENT | 2 | 3.2 | BODY OF DATA | 4 | 3.2.S | Drug Substance | 4 | 3.2.P | Drug Product | 5 | 3.2.P.1 | Description and Composition of the Drug Product | 5 | 3.2.P.2 | Pharmaceutical Development | 6 | 3.2.P.2.1 | Components of the Drug Product | 8 | 3.2.P.2.1.1 | Drug Substance | 8 | 3.2.P
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control group then the cut-off value of mean+2 SD were used (Table 4). Any OD value from each ELISA assay that equaled or exceeded the cut-off value was regarded as positive for leptospirosis infection and sensitivity (%) of the assay was calculated. Specificity (%) of the assay was calculated based on the number of control group below the calculated cut-off. The sensitivity of the serological assay‚ based on cut-off
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A simple‚ rapid‚ specific and highly sensitive spectrofluorimetric method has been developed for the quantification of dabigatran etexilate mesylate (DAB) in bulk and capsule dosage form. A linear relationship was found between fluorescence intensity and DAB concentration in the range of 0.01-1.0 μg/ml in DMSO as solvent at an emission wavelength of 391 nm after excitation at 334 nm‚ with a good correlation coefficient (0.989). The detection and quantification limits were found to be 0.005 and 0
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OUTLINE OF TOPICS IMMUNOLOGY AND SEROLOGY Dec 14‚ 2014 8:00 am – 12:00 nn Part 1: IMMUNOLOGY UNIT 1: INTRODUCTION 1. Historical Development 2. Definition of Terms UNIT II: IMMUNITY 1. Natural/Innate Immunity First line of Defense Anatomical/ Physical Barriers of Infections Second Line of Defense Physiological Barriers Biochemical Factors Cellular Factors Phagocytosis Third Line of Defense Immune response 2. Acquired/Adaptive Immunity Active Acquired Immunity Passive Acquired Immunity Humoral Immunity
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The AMES test also known as bacteria reversed mutation assay is used to evaluate the mutagenic properties of test articles. The test was first developed by Bruce Ames in 1974 (Krebsfaenger). The amino acid dependent strain of S. typhimurium and E. coli are used in this experiment where in the absence of the external histidine source‚ the cells cannot grow to form colonies. Specifically these strains of Salmonella are defective in 1.) Repair of mutations (uvrB) and 2.) A rfa mutation (eliminating
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The effect of food source (mung bean Vigna radiata vs. black-eyed pea Vigna unguiculata) on relative inhibition of acetylcholinerase due to malaoxon in bean beetle Callosobruchus maculatus Background and Hypotheses: Recent studies‚ most notably Gbaye et al. (2011‚ 2012)‚ have investigated the sensitivity of bean beetles in the genus Callosobruchus to organophosphate insecticides (OPs). Economically this is important work given that these beetles are pests that threaten agricultural yields
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nitrogen and stored at -80 ˚C after 0.22 μm pore membrane filtration. Evaluation of DNA-protein interaction by gel retardation assay Protein-DNA complexes were formed using 1 µg of plasmid pTriEx-1.1 Hygro at different pDNA: protein molar ratios (1:100‚ 1:200‚ 1:500‚ 1:1000‚ 1:2000‚ 1:8000) during 10 minutes. The increasing molar ratios were assessed by gel retardation assay on a 0.8% agarose gel and visualized by ethidium bromide staining. For further experiments‚ all samples were prepared in the
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This work is focused on evaluation of the potential anticancer effect of a kola nut extract on the human immortalized myelogenous leukemia line (K562) and the human immortalized line of T-lymphocyte cells (Jurkat). The objectives of this research are as follows: 1- Does kola nut extract inhibit leukemia? 2- Does the growth in inhibition induced by kola nut extract fellow a dose- and time- dependent response? 3- Does kola nut extract induce apoptosis or necrosis? 4- Which cell line‚ a K562 or Jurkat
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1. By using two specific examples of an infectious disease and cancer (lymphoma or leukemia‚ etc) describe the application of the following techniques in detection and diagnosis. a) Flow cytometry analysis “Feline Panleukopenia (FP) is a highly contagious viral disease of cats caused by the Feline Parvovirus‚” (American Veterinary Medical Association‚ 2013). This disease is a secondary infection that caused by the apoptosis of infected animal’s cells and reduces the expression of interleukin-2
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