Lastly‚ record the volume of the sodium hydroxide used on the table. 10. Repeat this procedure twice for each tablet type. Results Assay 1 Assay 2 Assay 3 Mass of tube + aspirin (g) 2.76g 2.77 g 2.75 g Mass of tube (g) 2.46g 2.46 g 2.46 g Mass of aspirin (g) 0.3g 0.31 g 0.29 g Initial burette reading (cm3) 20cm3 32.6 cm3 37.5 cm3 Final burette reading (cm3) 32.6cm3 47.5 cm3 47.8 cm3 Volume
Premium Aspirin Salicylic acid Acetic acid
Figure 3.1: Typical standard chromatogram of Ritonavir and Lopinavir Figure 3.2: Typical sample chromatogram of Ritonavir and Lopinavir 3.3.2 Method validation The optimized RP-HPLC method for simultaneous assay of Ritonavir and Lopinavir was validated according to ICH guidelines with respect to system suitability‚ linearity‚ sensitivity‚ accuracy‚ precision and robustness. 3.3.3 System suitability Before sample analysis‚ the chromatographic parameters used in this analysis must conform the system
Premium Analytical chemistry Chromatography
II.Results and Discussion A.Isolation of Agrobacterium tumefaciens: Six bacterial colonies were observed and screened on the selective medium. The bacterial colonies was able to characterized after 72 hours of incubation‚ bacterial colonies were visible with naked eyes on YEMA plates. The colonies appeared were medium sized bubble shaped‚ round‚ regular‚ sticky and white coloured colonies were observed. From these initial results‚ the isolated bacteria were tentatively identified as Agrobacterium
Premium Bacteria Microbiology Escherichia coli
for “Enzyme-linked Immunosorbent Assay." II. Introduction: The interaction of antigen and antibody outside the body can be used to determine if patient has an infectious or an autoimmune disease. In this particular exercise‚ the sample blood is collected from three different patients. And after‚ separating the red blood cells and serum‚ a serum was used to perform the ELISA test. a. ELISA Test ELISA is an abbreviation for “Enzyme-linked Immunosorbent Assay." An ELISA test uses components of
Premium Antibody Immune system ELISA
states: Oxford university press Inc. Sigma-Aldrich Corporation .LLC‚ 2013. Comet assay Available from: www.sigma aldrich.com‚ s.l.: s.n. The John Innes centres‚ 2013. The John Innes centres (What is electron microscopy). [Online] Available at: http://www.jic.ac.uk Tsai‚ C.‚ 2013. In situ hybridization Available from: www.ncbi.nlm.nih.gov‚ USA: Elsevier. Inc. Wochenschrift‚ W. K.‚ 2011. Application of the comet assay method in clinical studies Available from: link.springer.com. Volume 123‚ pp. 693-695
Premium DNA
Next‚ the “3D Skin Modle” a project developed using human reconstructed skin (RS) models with the combinations of micronucleus (MN) as well as Comet assays. These models reflect the use of conditions that dermally are applied to products‚ such as that of cosmetics. These two assays are going good and new stages of them are being created to make it that much better (Pfunder). “Metabolism” is the last of the three projects that investigated enzymes of the skin among
Premium Animal testing Animal rights Testing cosmetics on animals
Methods Antibacterial activity of aqueous and organic seed extracts was assessed using agar diffusion assay‚ minimum inhibitory concentration and viable cell count studies; and their antibacterial effect was compared with some standard antibiotics. The presence of major phytoconstituents was detected qualitatively and quantitatively. The isolated phytoconstituents were subjected to disc diffusion assay to ascertain their antibacterial effect. Results Hot water and acetone seed extracts showed considerably
Premium Fennel Apiaceae Alternative medicine
sensitivity is also low‚ ranging from 50-80%. Both methods are inherently limited because they rely on the organism to be viable for proper detection. These limitations have led to interest in development of molecular assays for the detection of T. vaginalis DNA from clinical specimens. PCR assays have been developed that are specific and show an improved sensitivity. With appropriate awareness of importance of this commonly neglected pathogen and with use of adequate diagnostic tests this parasitic organism
Premium Immune system AIDS Cancer
using the rat paw oedema assay‚ and for its in vitro IÀ scavenging effects on reactive oxygen species (ROS) (HOI‚ O2 ‚ HOCl‚ ROOI and H2O2)‚ reactive nitrogen species (RNS) (ONOOÀ and INO)‚ and I DPPH radical. The protein‚ free amino acid‚ and phenolic contents of the tincture were also determined. Pertaining to the anti-inflammatory activity‚ the intraperitoneal administration of the tincture inhibited carrageenan-induced rat paw oedema‚ whereas in the scavenging assays the IÀ tincture showed to
Premium Amino acid Antioxidant
UNIVERSITY OF DAR ES SALAAM COLLEGE OF NATURAL AND APPLIED SCIENCE Dept: Molecular biology and biotechnology Name: Ernest Medard Reg#:2010-04-00120 Degree program: Bsc. MBB BL 390: research project TITLE: production of Laccase and Pectinase enzymes by Schizophyllum spp and its application in dye decolorization Project supervisor: Dr. R. Masalu Lab scientist: Mr. Chuwa INTRODUCTION: Due to rapid industrialization and urbanization‚ a lot of chemicals including dyes are manufactured and
Premium Dye Enzyme