The antioxidant capacity of Bifurcaria bifurcata extracts from the Peniche coast was evaluated by the analysis of the total polyphenol content (TPC)‚ by the DPPH free radical scavenging activity and by Oxygen Radical Absorbance Capacity (ORAC). The results are expressed in Table 1. The methanolic fraction was more potent than the dichloromethane fraction‚ with an EC50 of 58.82µg/mL (50.65 – 68.31)‚ which was very similar with the standards‚ ascorbic acid and BHT‚ that exhibited an EC50 of 22.43µg/mL
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Lactate Dehydrogenase (LDH) is an cytosolic enzyme that participates in anaerobic glycolysis. LDH couples the reduction of pyruvate into lactic acid to the oxidation of NADH to NAD+‚ which allows glycolysis to continue to produce ATP in the absence of oxygen. LDH is a tetramer‚ a protein complex of 4 polypeptide subunits‚ composed either a subunit expressed strongly in the heart‚ H type‚or a subunit that is highly expressed in the muscle‚ M type. There are 5 isozymes of LDH composed the 2 subunit
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Giardiasis Disease Etiology: Giardiasis‚ also known as “traveler ’s diarrhea‚” [3] is caused by Giardia intestinalis. Other names for this parasite are Giardia lamblia‚ and Giardia duodenalis. There are many different genetic assemblages of this parasite‚ some that infect only mammals‚ some that infect primarily humans‚ and a few that will affect both animals and man. [1] Transmission: While G. intestinalis “can live in the intestines of animals and people” it is very rare for a human to
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between 1% and 21% every 8 minutes and 4 minutes‚ respectively. The cells were subjected to IH for a total of 12 hours‚ consisting of 60 cycles. Following this‚ the model cells were co-incubated with various nanoparticles for 4 hours‚ and the CCK-8 assay was used to detect changes in cell viability. Subsequently‚ the cells were co-incubated with different concentrations of PDA@MT for 4 hours and then stained using a live/dead staining kit to assess the effect of varying nanoparticle concentrations
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TITLE AND AUTHOR Lab 7 Analysis of purified Concanavalin A via:Hemagglutination INTRODUCTION The purpose of this lab was to test the biological activity of ConA by performing a hemagglutination assay. If ConA is active then agglutination will occur due to ConA’s free receptors being able to bind to the glucose residues on the sheep’s red blood cells. If ConA is not active then no agglutination will occur. To test the hemagglutination reaction‚ two types of ConA solutions
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Bacon once said: “Knowledge is power.” In our present day society‚ we are fortunate to have developed resources that greatly improve the quality of our lives. For example‚ technology has advanced to the point where we are able to perform genetic assays. These tests detect changes in chromosomes‚ genes‚ and proteins to help identify the likelihood of a child being born with different genetic conditions. Personally‚ I believe this test proves to be highly beneficial because it educates couples who
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The United States is the land of opportunity and competition‚ so in order for robotics in healthcare to succeed it must make sense financially. Robot-assisted surgery is sometimes considered to be the most expensive form of surgery due to the cost of the robotic system‚ but that is not true when the indirect costs of robot-assisted surgery are also considered. Landeen et al. studied the cost effectiveness of the four methods of hysterectomy‚ and they concluded robot-assisted hysterectomy was the
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CHAPTER 1 Coagulation Pathway and Physiology Jerry B. Lefkowitz‚ MD Introduction Our understanding of blood clotting is intimately tied to the history of civilization. With the advent of writing 5000 years ago‚ it could be argued that the first symbols used for blood‚ bleeding‚ or clotting represented the first published coagulation pathway. The ancient peoples of the world always held blood in utmost mystical esteem. Through the ages‚ this esteem has been transmitted to modern times in the
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cell lysis was determined using the weight of the pellet. The pellets were then suspended in B-cell lysis and incubated for 15 min at room temperature. The concentration of cell lysate was determined using a Bradford assay and the samples were diluted accordingly. The Bradford assay was obtained by shining a light with a wavelength of 405 nm through the cell lysate sampled. The data obtained was absorbance and was then plotted vs. the concentrations of the samples. The line
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and Catto‚ J.W.F. (2008) ’Haematuria’‚ Surgery‚ 26(4)‚ pp. 150-153. Flores‚ R. (1978) ’A rapid and reproducible assay for quantitative estimation of proteins using bromophenol blue’‚ Analytical Biochemistry‚ 88(2)‚ pp. 605-611. Meurman‚ J.H.‚ Rytömaa‚ I.‚ Kari‚ K.‚ Laakso‚ T. and Murtomaa‚ H. (1987) ’Salivary pH and glucose after consuming various beverages‚ including
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