affect cell viability during the lab experimentation one more test was conducted because the first experiment was done with a much more concentration of caffeine. This resulted in killing most of the cells and run another experiment of the trypan blue assay with lower concentrations of caffeine. The second experiment
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distilled water (sample free-disc). From the Microbiological Assay Plate Testing‚ the zone of inhibition was determined. The collection of Sambong leaves‚ Onion and Takip Kuhol leaves was grinded and was submerged in a 95% ethyl alcohol. After 24 hours of submersion‚ the treatments were put into water bathing for the evaporation of ethyl alcohol. The extract produced was then brought to the testing center and was tested through a Microbiological Assay Plate Testing. The zones of inhibition were determined
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Analysis of Glycated Hemoglobin by HPLC Technology Shalini Kini Product Specialist Diabetic EpidemiologyWhere Do we Stand!?! World Scenario * IDF Diabetes Atlas 5th Edition 2012 update. http://www.idf.org/diabetesatlas/5e/Update2012 Did U Know!!??! One Adult in Ten will have diabetes by 2030!!! Year 2012 Year 2030 63.01m India 101.2m India • India Ranked Second in the world in Diabetes Prevalence‚ behind China. *IDF Diabetes Atlas- Fifth Edition http://www.idf
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TITLE: The Amount of Protein in Chicken Tissues over Cooked Various Periods of Time. ABSTRACT: In this lab‚ we are using a BioRad protein assay dye to determine the concentration of protein in our chicken. The dye binds to the amino acid residues‚ which allow us to find the concentration of protein (BioRad Protein Assay for Tissues). Our hypothesis was the longer chicken is cooked the less protein is available. To test our hypothesis‚ we made samples using our chicken and distilled water to determine
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2.3. Extraction of the flavonoids fraction of Cajanus cajan (FFCC) The Cajanus cajan L. seeds were air dried at 10% moisture‚ then powdered and preserved in a refrigerator at -80°C. Five hundred gm from the powdered seeds were defatted with hexane‚ then extracted with methanol (MeOH - 70%) four times‚ then concentrated under vacuum to yield 37 g. Polyamide and sephadex LH-20 chromatography columns were used to fraction the MeOH (70%) extract of Cajanus cajan. Seven flavonoid fractions were collected
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synthesized novel naphthoquinone aromatic amides and evaluated for anticancer activity. Benzamide 80 showed potent inhibition against NCI-H187 cell lines while naphthamides 81 and 83 were the most potent inhibition against KB cells. The decatenation assay revealed that compounds 82 and 83 inhibit hTopoIIa activity at 20 µM‚ while three other compounds 81‚ 82‚ and 84‚ exhibited hTopoIIα inhibitory activity at concentration of 50 µM [53]. Novel oxo-heterocyclic fused naphthalimide derivatives were prepared
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solution. To establish a baseline‚ start by putting 10 mL of 1.5% H2O2 into a clean glass beaker. Add 1 mL of H2O (instead of enzyme solution). Add 10 mL of H2 SO4 (1.0 M). Mix well. Remove a 5-mL sample. Place this 5-mL sample into another beaker and assay for the amount of H2O2 as follows. Use a syringe to add KMnO4 ‚ a drop at a time‚ to the solution until a persistent pink or brown color is obtained. Remember to gently swirl in the solution after adding each drop. Check to be sure that you understand
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Experiment: DNA damage and mutations in human cells when exposed to nitric oxide Aim: To examine mutations after in vitro exposure of nitric oxide to human cells. Abstract: Nitric oxide (NO) is a anatomical carrier formed by various cell types. In this experiment nitric oxide is made to react with undamaged human cells and solutions of DNA‚ RNA ‚ guanine or adenine in aerobic conditions. TK6 human lymphoblastoid cells were altered. It has been observed that deamination of purines‚ pyrimidines
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Objectives This experiment aims to analyse the given sample of acetaminophen‚ Sample A‚ to see if it complies with the monograph in the British Pharmacopoeia (BP) for the identification of acetaminophen‚ the limit test for p-aminophenol and the assay for acetaminophen. Procedure Identification for Acetaminophen Potassium bromide (KBr) disc technique was used to prepare the sample for the infrared absorption spectrophotometry. The agate mortar and pestle were cleaned with absolute ethanol using
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1. What are antibodies and why are antibodies ideal for targeting? An antibody‚ also known as an immunoglobulin‚ is a large Y-shaped protein used by the immune system to identify and neutralize foreign objects such as bacteria and viruses. The antibody recognizes a unique part of the foreign target‚ termed an antigen.[1][2] Each tip of the "Y" of an antibody contains a paratope (a structure analogous to a lock) that is specific for one particular epitope (similarly analogous to a key) on an antigen
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