tissue using a variety of various. Analytical methods such as activity and protein assay were employed to determine the presence and purity of LDH. The cells were initially disrupted and proteins were solubilized. LDH was purified from the ammonium sulfate precipitated protein mixture by affinity chromatography and its activity was studied by spectrophotometric determination of NADH at 340 nm. From Pierce BCA assay of crude homogenate‚ initial protein concentration was shown to be 100 mg/ml. The
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roughly three times that of the printed label with a value of 100.2 mg/ml; cereal milk was twice that of its printed label with a value of of 84.7 mg/ml and muscle milk was found to have a higher printed label than the measure protein concentration from assay with a value of 35.8 mg/ml. It can be observed that measured values were significantly greater than printed label values (Table 5). These inconsistencies can be caused by multiple factors. Errors such as not adding enough dye reagent‚ incorrect dilution
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Cyclooxygenase-1 and -2 in Vitro Assay for Identification of Natural Products as Inhibitors of Prostaglandin Biosynthesis Ylva Noreen‚† Therese Ringbom‚† Premila Perera‚† Helena Danielson‚‡ and Lars Bohlin*‚† Division of Pharmacognosy‚ Department of Pharmacy‚ Biomedical Centre‚ Uppsala University‚ Box 579‚ S-751 23 Uppsala‚ Sweden‚ and Department of Biochemistry‚ Biomedical Centre‚ Uppsala University‚ Box 576‚ S-751 23 Uppsala‚ Sweden Received July 18‚ 1997X A radiochemical enzyme assay for studying cyclooxygenase
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Hi Robert‚ I enjoyed your information on Luis Pasteur. He was most definitely an amazing microbiologist. I must agree with you that his contributions to science and the world are without question remarkable and through your presentation of this scientist‚ I was reminded of studying him in my 10th grade biology class many many years ago. In addition to all the contributions you mentioned‚ I believe Luis Pasteur could also be considered the founder of medical microbiology who broke the germ
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of the other unknown protein solutions can be determined (Lambert et.al‚ 2011).The different assays used for this protein quantification were Lowry‚ Bradford (Coomassie Blue) and UV direct. Protein assays help to determine the amount of desired particle
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from E. coli. This enzyme hydrolyzes lactose and turns it into galactose and glucose. Since it is difficult to assay the activity of β-galactosidase‚ we will be using the artificial substrate‚ o-nitrophenyl-β-galactoside (ONPG) instead of lactose. ONPG is an analog of lactose and an advantage of using ONPG is that it is easy to determine the amount of ONPG cleaved by using spectrometric assay (1). The β-galactosidase hydrolyzes ONPG and yields a yellow solution that contains o-nitrophenol and galactose
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Proximate and Phyto Chemical Screening of CISSUS POPULNEA ONOJAH‚ P.K.‚ 2SALAWU‚ O.W.‚ AND 3UMAR .S. CHEMISTRY DEPARTMENT‚ KOGI STATE UNIVERSTIY‚ P.M.B. 1008‚ ANYIGBA ‚ KOGI STATE. NIGERIA. Abstract The work reports the chemical evaluation‚ nutritional and flavoring properties of Cissus Populnea. This spices contain crude protein (37% - 21%)‚ crude fibre (23 — 22%)‚ crude fat (3.10 — 20%)‚ carbohydrate (16 — 24%) total ash content (2.0 — 3. 10%)‚ Moisture (1.68 — 1.82%). The spices are sources of
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COAL GRADING IN INDIA: The gradation of non-coking coal is based on Useful Heat Value (UHV)‚ the gradation of coking coal is based on ash content and for semi coking / weakly coking coal it is based on ash plus moisture content ‚ as in vogue as per notification. Grades of Coking Coal Grade Ash Content Steel Grade –I Not exceeding 15% Steel Grade -II Exceeding 15% but not exceeding 18% Washery Grade -I Exceeding 18% but not exceeding 21% Washery Grade -II Exceeding
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ANALYTICAL BIOCHEMISTRY Analytical Biochemistry 334 (2004) 196–198 www.elsevier.com/locate/yabio Notes & Tips A spectrophotometric assay for the quantiWcation of polyethylenimine in DNA nanoparticles Martin Bertschinger‚ Sophie Chaboche‚ Martin Jordan¤‚ Florian M. Wurm Swiss Federal Institute of Technology Lausanne‚ Laboratory of Cellular Biotechnology‚ Lausanne VD1015‚ Switzerland Received 29 April 2004 Available online 28 August 2004 Since the Wrst description of polyethylenimine (PEI)1 as a
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Title: Preparation and Assay of Phenolase and Peroxidase from Sweet and Irish Potato Aim To design and conduct an experiment to demonstrate the presence of enzyme activity in the preparation provided. To examine the effect of the inhibitors provided. To test whether the other phenolic substrates provided can be oxidized by the enzyme preparation. To test for the presence of peroxidase activity in the enzyme preparation. To test the effect of the inhibitor provided on peroxidase activity
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