Nur fateha rasul Good morning to our english teacher Madam Mohana . Today ‚ we want to discuss about living in countryside is better than living in city . There are many points that we can discuss ‚ such as Living in countryside is healthier than living in city Living in countryside our social is better than living in city Cost living in countryside cheaper than living in city Living in countryside is more safety than living in city That’s all from me . Thank you . Nurul
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Alamar Blue assay are performed to show cell viability and proliferation. In the procedure we treated our cell line‚ SUM 149 cells with different concentrations of an unknown treatment. SUM 149 cells are an inflammatory breast cancer cell line. The treatments were used to suppress cell growth and proliferation. The station that I performed my work was station C. I platted my cells and aloud a previously determined allotted time for cell proliferation and growth to occur. The cells were then treated
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The reaction between alpha-amino acid and ninhydrin involved in the development of color are described by the following five mechanistic steps: alpha-amino acid + ninhydrin ---> reduced ninhydrin + alpha-amino acid + H2O alpha-amino acid + H2O ---> alpha-keto acid +NH3 alpha-keto acid + NH3 ---> aldehyde + CO2 Step (1) is an oxidative deamination reaction that removes two hydrogen from the alpha-amino acid to yield an alpha-imino acid. Simultaneously‚ the original ninhydrin is reduced
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A.‚ Cassin‚ P.E.‚ & Gambier‚ R.M. (2000). Biotechnology Explorations: Applying the Fundamentals. Page 157-158. Washington‚ DC: ASM Press. [4] Bradford Method. (2012). Retrieved July 14‚ 2012 from‚ http://www.scribd.com/doc/51367139/Protein-Assay-Using-Bradford-Method [5] BSA standards (2012). Retrieved July 16‚ 2012 from‚ http://www.piercenet.com/browse.cfm?fldID=02020108
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casein. Activity assay for invertase was performed using Benedict’s test and the enzymes inverting action on sucrose was confirmed. Warburg-Christian Method and Bradford Assay were also employed to determine the protein concentration in the albumin and the casein samples. The concentrations for the albumin and casein samples were found to be 0.519 and 0.327 mg/mL‚ respectively based on Warburg-Christian Assay; and 6.5x10-3¬ and 1.9x10-2 mg/mL based on Bradford Assay. In general‚ the assay indicates that
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Title: Isolation and purification of bovine milk α-lactalbumin. Abstract: The Bradford assay‚ a colorimetric protein assay‚ is based on an absorbance shift of the dye Coomassie Brilliant Blue G-250 in which under acidic conditions the red form of the dye is converted into its bluer form to bind to the protein being assayed. The (bound) form of the dye has an absorption spectrum maximum historically held to be at 595 nm. Objective: To determine the technique to a series of protein purification
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Expression and Purification of Human Platelet-Derived Growth Factor Beta (PDGF-B) Rachel C. Hermina-Stewart* and Elsie I. Parés-Matos† *Industrial Biotechnology Program‚ †Department of Chemistry University of Puerto Rico-Mayagüez Campus rachel.hermina@upr.edu December 14‚ 2009 ABSTRACT. Platelet-Derived Growth Factor (PDGF) is one of many proteins that regulate cellular growth and division. The PDGF family consists mainly of five different isoforms called as PDGF-A‚ PDGF-B‚ PDGF-C and PDGF-D. Three
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Results 5-6 Discussion 7-8 Conclusion 9 References 9 Introduction The concentration of protein in a sample can be obtained by using the Bradford protein assay method. The method requires a spectrophotometer and it is easily operated. The Bradford protein assay is a colorimetic assay based on the interaction of the blue dye “Coomassie” and the protein. The blue ye firsts donates free electron to the protein and causes the protein to lose its native state and exposing
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ELISA (Enzyme-Linked ImmunoSorbant Assay) ELISA is abbreviated term for Enzyme-Linked ImmunoSorbant Assay. This procedure is one of the most widely used methods in clinical immunology assays to detect the presence and absence of certain antigens or antibodies and also to quantify them when necessary. Quantification can be done in a range of microgram (µg) to nanogram (ng). The ELISA procedure takes advantage of the fact that most proteins will bind firmly to the surface of different kinds
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Main aim of experiment 5 is to use the biuret test to quantify the concentration of the Bovine Serum Albumin (BSA) then constructing a standard curve graph and to use the spectrophotometer to perform an enzyme assay using different concentration of the BSA. Experiment 5 also verifies the Beer-Lambert Law‚ which is the linear relationship between absorbance and concentration of an absorbing species. Absorbance formula is shown in fig. 1.1. However‚ the Beer-Lambert Law is not obeyed at high concentration
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