In this report‚ the concentration of total protein and albumin were calculated with the interpretation of a standard curve. To determine the concentration of total protein in human serum‚ a total protein assay was performed by using the Biuret reagent. Proteins react to this compound because it contains two amide bonds. When the Biuret reagents is introduced to a sample of protein‚ it forms a blue or violet complex. The concentration of albumin in human serum is determined by using the Bromocresol
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also be used to defat the protein. Lastly‚ the solution undergoes differential centrifugation. This will separate the protein crude from the liquid. (Campbell) After protein purification‚ the crude now undergoes characterization. Activity assay‚ Bradford assay‚ and Warburg-Christian method can be used to characterize proteins. Spectrophotometric analysis are usually used to determine some properties of the protein such as protein concentration. Results Discussion A. Invertase from yeast The
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to use the Bradford and DC Protein Assay in order to determine our concentration of E. Coli BL21(DE3) lysate. Principle of Methods: To accomplish this objective‚ each pair of lab partners received an E. Coli lysate sample which we had to thaw by warming it with our hands and centrifuge it. We extracted a total of 12 samples with known concentrations of bovine serum albumin and 10 samples of different amounts of lysate put into them. Using the different assays explained later in this report‚ we
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Bovine Serum Albumin (BSA) standard curve and observe how the protein concentration of BSA and Lysozyme is affected by the addition of detergents‚ Sodium Dodecyl Sulphate (SDS) and Triton X-100. This study involves two procedures. Firstly a protein assay needs to be carried out in order to investigate the
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Uday J Vyas Dr. Colbert Dennis ENG 106 NLM 20th July 2013. This assay compares and contrasts between two main fields of studies which are Arts stream and Science stream and reflects on similarities and distinguishing features of respective fields of studies. Both of these fields of studies have wide scope of learning through various branches. Arts offers literary arts‚ more often called literature – including poetry‚ novels and short stories among others and performing arts
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microplate reader is to measure the absorbance at 595 nm. The data collected needs to be plotted on a gridline-based graph and the standard curve is a line of best fit that is equally spaced in-between the highest and lowest data points. The Bradford assay is performed three times in succession (in triplicate) for Wash 1-6 and Elution 1-6. The absorbance value will be extrapolated from the standard curve created on the gridline-based graph. The total amount of protein in ug that was present in the 0
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counting oxygen bubbles generated by an elodea stem tend to not be “student” proof or reliable. This is a particular problem if your laboratory instruction emphasizes student-generated questions. Over the years‚ I have found the floating leaf disk assay technique to be reliable and understandable to students. Once the students are familiar with the technique they can readily design experiments to answer their own questions about photosynthesis. The biology behind the prodedure: Leaf disks float
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Enzyme assay lab report Health and safety: 4-nitrophenol is harmful. Introduction: Enzymes are quaternary structured proteins that are specific biological catalysts that speed up a reaction without being used up. They contain an active site that allows substrate to bind to a specific area on the enzyme which is of a complimentary shape of the substrate. There are two models of enzyme action‚ the Lock and Key model and the Induced Fit model. The Lock and Key model states that the enzyme has a specific
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Proteus vulgaris Antibiotic Sensitivity Assay By: David Toelkes BIO 225 June 12‚ 2012 Lab Partners Alexis Blackmon Cheryl Cardiff Megan Dereani Logan Freeman Abstract Development of bacterial resistance to the antimicrobial drugs identified the need to determine a given bacterium’s susceptibility or resistance to a given drug which prompted W. M. M. Kirby and A. W. Bauer to develop a single disk method for susceptibility testing. This experiment used the Kirby-Bauer disk diffusion
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Discussion: The liver homogenate was expected to have the highest amount of protein concentration‚ DNA concentration‚ and total glucose when compared to the other homogenates. The liver was the biggest organ in B. taurus and it carried out numerous functions such as producing bile‚ synthesizing proteins for blood clots‚ and recycling old red blood cells. The liver ended up containing the most DNA concentration‚ but it was surpassed in protein concentration by the kidney. The protein concentration
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