Cytotoxic and Genotoxic Effects of Gutkha using Allium cepa Assay EKTA BHATTACHARYA Roll BGC/BOT No. 112001 M.Sc Semester IV Examination 2013 Under the supervision of Dr. Shyamal Kr. Chakraborty Associate Professor‚ Cytogenetics Laboratory‚ PG Dept of Botany‚ Barasat Government College Cytotoxic And Genotoxic Effects of Gutkha using Allium cepa Assay EKTA BHATTACHARYA Roll BGC/BOT No. 112001 M.Sc Semester
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Sensors 2009‚ 9‚ 6084-6100; doi:10.3390/s90806084 OPEN ACCESS sensors ISSN 1424-8220 www.mdpi.com/journal/sensors Article From Lateral Flow Devices to a Novel Nano-Color Microfluidic Assay Saied Assadollahi 1‚2‚ Christiane Reininger 1‚3‚ Roland Palkovits 1‚3‚ Peter Pointl 1 and Thomas Schalkhammer 1‚2‚* 1 2 3 Attophotonics Biosciences GmbH‚ Viktor Kaplan Strasse 2‚ A-2700 Wiener Neustadt‚ Austria; E-Mails: palkovits@attophotonics.com (R.P.); Reininger@attophotonics.com(C.R.); pointl@attophotonics
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Title of Lab: Preparation and Assay of Phenolase and Peroxidase from Sweet and Irish Potato Hypothesis: Polyphenol Oxidase enzyme activity can be detected by change in colour of solution‚ Inhibitors prevent the reaction of the enzymes with substrates‚ the enzyme is relatively specific. Aim: To design and conduct an experiment to demonstrate enzyme activity of Polyphenol Oxidase and Peroxidase when mixed with catechol‚ caffeic acid‚ pyrogallad‚ tyrosine‚ guacol‚ and water‚ to test the effect of
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SOS—A Sample Ordering System for Delivering “Assay-Ready” Compound Plates for Drug Screening Biochemistry and Molecular Biology‚ Merck Frosst Centre for Therapeutic Research‚ 16711 TransCanada Hwy.‚ Kirkland‚ Quebec‚ Canada H9H 3L1; Phone: +1.514.428.3360; E-mail: christine_brideau@merck.com Abstract Many bottlenecks in drug discovery have been addressed with the advent of new assay and instrument technologies. However‚ storing and processing chemical compounds for screening remains
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Journal of Infection (2011) xx‚ 1e7 www.elsevierhealth.com/journals/jinf Improving the timeframe between blood collection and interferon gamma release assay using T-Cell XtendÒ J.J.M. Bouwman a‚*‚ S.F.T. Thijsen a‚ A.W. Bossink b a b Department of Medical Microbiology and Immunology‚ Diakonessen Hospital Utrecht‚ The Netherlands Department of Pulmonology‚ Diakonessen Hospital‚ Utrecht‚ The Netherlands Accepted 9 October 2011 T-Cell XtendÒ; TSPOT.TBÒ; Lithium heparin; IGRA; Interferon
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ASSAY OF OIL-DEGRADING POTENTIAL OF FUNGI ISOLATES ON DIESEL‚ KEROSENE AND PETROL USING ENRICHMENT METHOD. BOBOYE B.‚ *OLUKUNLE O. F.‚ ADETUYI F. C. AND ADEBIYI G. A ABSTRACT A study was carried out to assay for oil-degrading potential of fungi isolates on diesel‚ kerosene and petrol. Water samples were collected aseptically and analyzed microbiologically using standard techniques. The fungi isolated from the water samples were: Trichoderma viridae‚ Aspergillus niger‚ Aspergillus fumigatus
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Approximately 2 x 104‚ 5 x 104‚ 2 x 10 5 and 5 x 105 cells were seeded in 96‚ 24‚ 12‚ and 6 wells tissue culture plates respectively‚ and incubated at 37ᵒC in 5% CO2 for 24 h‚ preceding cell viability assay or
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0.5% violet crystal in 20% ethanol. The percentage of viral inhibition (%VI) was calculated by the following formula according to Nishimura‚ Toku and Fukuyasu (1977) [18]: %VI = 1 – [(PFU in treated cells/PFU in virus control)] x 100. Virucidal assay: A virus suspension (105 PFU mL-1) containing varying concentrations of the samples as above was incubated at 37°C in a water bath for 1 hour. Subsequently‚ the suspension was added to the cells (0.1 mL per well) and incubated again at 37°C for 1 hour
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2. Experimental 2.1. Experimental procedure for the synthesis of bone-like CS-PEG-HA-ZrO2 nanocomposites (BNC I-III). At first‚ ZrO2 nanoparticles (NPs) were synthesized by using the previously reported method [16]. Briefly‚ aqueous solution of sodium hydroxide (purchased from HiMedia Private Ltd.) (40 ml 0.05M) was slowly added in zirconium oxychloride octahydrate (purchased from Merck India Ltd.) solution (100 ml 0.01M) prepared in methanol-water (1:1) at 5 oC temperature. The mixture was stirred
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consequential preparation of CM Coating of plates for EPC culture Mononuclear cell isolation from human peripheral/ umbilical cord blood EPC- medium changes for CM incubation Collection of EPC-CM Immunohistochemical stainings of EPC Proliferation Assays 3.5.a. Presto Blue
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