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In the first part of this lab‚ E.coli cells were transformed with an R-plasmid carrying a tetracycline resistant gene‚ giving rise to tetracycline resistant E.coli strain. This was accomplished through transformation‚ which allowed E.coli to directly uptake the naked DNA molecule carrying the antibiotic resistant gene (1). However‚ in order to take up the DNA and incorporate them into their genome via recombination‚ cells must be competent (1). Therefore‚ E.coli cells which are not competent under
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Gisselle A. Clarito BSFS-103 “THE FIRST MET” It was the first day‚ and the first year of Sandra’s life of being college. She was a kind of nervous walking along the lobby on that day. She can’t find a friend who is fitted to her. But‚ unfortunately‚ she met a friend name Andrea. Andrea was a first year student also and a Psychology student in Clarito National University. They become friends since they met. Then‚ on that time‚ Andrea and Sandra become confident on introducing their selves on
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These two English Translations of the Russian poem by Andrei Voznesenky are about a girl being rejected for the first time. The young girl’s feelings of hurt and rejection are described in terms of coldness throughout the poem. “The first ice of human hurt”. The theme that is put across from the poem is of growing up‚ and the experience of loosing someone for the first time. Both of these poems are very similar‚ but they have both been translated in two different styles. This helps to alter the meaning
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The first and last Dan Savage‚ an American writer‚ once stated‚ “Ultimately life is disease‚ death and oblivion. It’s still better than high school” (Savage). This quote represents what my high school career was like; for example‚ I have been to 4 different high schools. I was in foster care‚ so I would move around a fair amount. Moving around‚ I experienced many first and last experiences in high school. Despite what Savage stated‚ not all of my first and last experiences in high school were horrible
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anyways. I was pretty nervous. On the first day of school‚ my mom woke me up by turning on my light and said “first day of school! Wake up! I then went downstairs and quickly ate breakfast. Then I raced upstairs to my room to get dressed‚ followed by brushing my teeth. All I could think about was that it’s my first day of middle school. The more and more I thought about it‚ the more butterflies I could feel in my stomach. Before I left‚ I brushed my dark‚ straightened hair‚ and said “bye mom
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this term has defined my entire life over the past four years. My first taste of high school was in the spring of my 8th grade year. My band director hosted a marching band clinic for rising freshman. Fresh off of a personal victory of being awarded the Director’s award for Musical Excellence‚ I eagerly attended. After getting the taste of high school marching band‚ earning a spot on the snare line my freshman year‚ and getting my music down‚ I was ready to march my first steps on the field.
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In first grade‚ it was my first year in public school so I didn’t have as much friends as the the people that were in kindergarten last year. When I walked into the room‚ both of my parents on each of my side they introduced to my teacher then I sat in the empty seat. The person assigned to sit next was a girl named Chloe. At first sight‚ I mesmerized by the amount of silly bands she had. At that moment I knew I had to be her friend. Half way into the semester she started treating me badly. I would
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My first culture shock experience was 4 years ago when I came in to this country. It is very diverse here in America. There are many cultures and people from around the world. In my country the ratio is about ninety percent Turkish and My first culture shock experience was 4 years ago when I came in to this country. It is very diverse here in America. There are many cultures and people from around the world. In my country the ratio is about ninety percent Turkish and theMy first culture shock experience
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only thing running through my head as I attempted to complete the last half mile of my first cross-country meet‚ and at least it was running. I on the other hand‚ was not. I had started the race completely confident in my ability to run three miles‚ but after a grueling two and a half laps of tripping over tree roots‚ splashing through mud‚ and struggling over an enormous hill that marked the last quarter of the lap‚ I was just ready to go home and take a shower. When my feet crossed onto the threshold
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