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In the first part of this lab‚ E.coli cells were transformed with an R-plasmid carrying a tetracycline resistant gene‚ giving rise to tetracycline resistant E.coli strain. This was accomplished through transformation‚ which allowed E.coli to directly uptake the naked DNA molecule carrying the antibiotic resistant gene (1). However‚ in order to take up the DNA and incorporate them into their genome via recombination‚ cells must be competent (1). Therefore‚ E.coli cells which are not competent under
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The first and last Dan Savage‚ an American writer‚ once stated‚ “Ultimately life is disease‚ death and oblivion. It’s still better than high school” (Savage). This quote represents what my high school career was like; for example‚ I have been to 4 different high schools. I was in foster care‚ so I would move around a fair amount. Moving around‚ I experienced many first and last experiences in high school. Despite what Savage stated‚ not all of my first and last experiences in high school were horrible
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These two English Translations of the Russian poem by Andrei Voznesenky are about a girl being rejected for the first time. The young girl’s feelings of hurt and rejection are described in terms of coldness throughout the poem. “The first ice of human hurt”. The theme that is put across from the poem is of growing up‚ and the experience of loosing someone for the first time. Both of these poems are very similar‚ but they have both been translated in two different styles. This helps to alter the meaning
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anyways. I was pretty nervous. On the first day of school‚ my mom woke me up by turning on my light and said “first day of school! Wake up! I then went downstairs and quickly ate breakfast. Then I raced upstairs to my room to get dressed‚ followed by brushing my teeth. All I could think about was that it’s my first day of middle school. The more and more I thought about it‚ the more butterflies I could feel in my stomach. Before I left‚ I brushed my dark‚ straightened hair‚ and said “bye mom
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this term has defined my entire life over the past four years. My first taste of high school was in the spring of my 8th grade year. My band director hosted a marching band clinic for rising freshman. Fresh off of a personal victory of being awarded the Director’s award for Musical Excellence‚ I eagerly attended. After getting the taste of high school marching band‚ earning a spot on the snare line my freshman year‚ and getting my music down‚ I was ready to march my first steps on the field.
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My first culture shock experience was 4 years ago when I came in to this country. It is very diverse here in America. There are many cultures and people from around the world. In my country the ratio is about ninety percent Turkish and My first culture shock experience was 4 years ago when I came in to this country. It is very diverse here in America. There are many cultures and people from around the world. In my country the ratio is about ninety percent Turkish and theMy first culture shock experience
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The first day of tryouts at my new high school was going extremely well. Socializing on the sideline‚ I thought‚ “Maybe high school isn’t going to be that bad after all.” However‚ The last drill of the day destroyed my hopes for making the lacrosse team. Running toward the goal‚ I felt a massive pop in my hip. A ligament had torn‚ and I could hardly walk. For weeks as I hobbled along on my cane‚ I would catch people staring at me in the hallways. Whether the cane or just low self-confidence‚ I never
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In first grade‚ it was my first year in public school so I didn’t have as much friends as the the people that were in kindergarten last year. When I walked into the room‚ both of my parents on each of my side they introduced to my teacher then I sat in the empty seat. The person assigned to sit next was a girl named Chloe. At first sight‚ I mesmerized by the amount of silly bands she had. At that moment I knew I had to be her friend. Half way into the semester she started treating me badly. I would
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after our first game‚ when I caught my first touchdown pass. This experience was so special because I realized that I had what it took to be a great player‚ and that I needed to work hard to get there. I first fell in love with football watching it with my dad when I was young. After sitting down to watch a few games‚ I was hooked. My dad and I would go out in the backyard
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