2/15/2013 background on transformation of bacteria with pGLO plasmid Experiment #5 Aim: Purpose of this lab is to have plasmid activity transformed Material: Bacteria starter plate‚ pGLO DNA Plasmid‚ microcentrifuge tubes‚ Ice‚ water bath‚ CaCl2 Transformation solution‚ (LB) agar plate‚ (LB/Amp) agar plate‚ (LB/Amp/ara) agar plate‚ Micropipette‚ and Micropipette tips. Method: Genetic transformation is a procedure which is done by taking genes from one organism and putting them in another organism
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What’s in a Medicine? (WM) In this document you will find the following material to cover the new AS specification with your students. Notes for students and teachers Revised Chemical Ideas 13.4 part b Answers to problems for 13.4 part b Revised activity WM3 Answers to questions for revised activity WM3 Revised check your notes Revised map of the unit 2 3 6 7 9 10 11 WM © Harcourt Education Ltd 2004 Salters Advanced Chemistry These pages have been downloaded from www.heinemann.co.uk/science 1 Notes
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of increasing atomic number with the elements having similar properties placed in vertical columns is known as periodic table. MATERIALS/REAGENTS AND EXPERIMENTAL PROCEDURE For the Group IA & IIA Elements‚ each small amount samples ofLi2CO3‚ Na2CO3‚ K2CO3‚ MgCO3‚ CaCO3 and BaCO3 was place in the different test tubes. After placing them in the test tubes‚ we identified the physical state‚ color‚ and appearance of each. Then we test the solubility of each sample by using water. For the Group IVA
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Chloride Sodium Chloride Strontium Chloride Procedure: 1. Put on safety goggles 2. Wear gloves when handling chemicals 3. Obtain a 250 – mL beaker half-full with water. Obtain 6 wooden splints 4. Obtain six weighing dishes labeled “CaCl2”‚ “CuCl2”‚ “LiCl”‚ “NaCl”‚ “KCl”‚ “SrCl2” – each containing a small scoopful of each metallic solid in the corresponding weighing dish 5. Put 10 drops of alcohol on the solid in the watch glass 6. Light a match – wooden splint – watch glass. Observe
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was observed in the reaction(actual yield)‚ divided by the mass of the precipitate that was hypothesized in the calculations (theoretical yield). This number was multiplied by a hundred to determine the percent yield (equation 4). Equation 1: Na2CO3 (aq) + Ca(NO3)2 (aq) CaCO3 (s) + 2NaNO3 (aq) Equation 2: n = OR mole = Equation 3: Percent Yield = × 100 Hypothesis: The law of conservation of mass states that the mass of the reactants must be equal to the mass
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Topic 10 Rate of Reaction Part A Unit-based exercise Unit 36 An introduction to rate of reaction Fill in the blanks 1 concentration; time 2 instantaneous 3 a) the gaseous product / carbon dioxide evolved b) loss in mass 4 a) colorimeter b) standard alkali c) the gaseous product / carbon dioxide evolved 1 time to reach the opaque stage 5 transmittance; 6 hydroxide; titrimetric True or false 7 T In the oxidation of methanoic acid by bromine‚ the intensity of the orange colour of bromine
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DESIGN OF AN ABSORBER Problem statement To design an absorber (packed tower) for the absorption of 785.15 kg/h of sodium metabisulphite (Na2S2O5) at an average temperature of 68˚C and Y atm pressure with sulphur dioxide gas counter-currently reacting with Na2SO3 rich feed. Introduction Absorbers are vertical towers used in distillation‚ gas absorption and liquid-liquid extraction processes. Absorbers can be operated co-currently or counter currently. In the former‚ both fluids involved are fed
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Experimental Objectives In experiment #1 the main objective is to test the solubility and the strength of the ionic compounds that are formed during titration. Solubility is a measure of the degree at which a compound is able to dissolve. Titration is used in this experiment to determine the Ksp of the saturated solution of Mg(OH)2 Ca(OH)2 SR(OH)2. Procedure and Observations Saturated solutions of Mg(OH)2‚ Ca(OH)2‚ and Sr(OH)2 are all prepared in which they need to be diluted with distilled
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contains four sections‚ SECTION A to SECTION D. Instructions: Answer all questions in SECTION A - SECTION D. Make sure that the section heading is included and your answers are correctly numbered. The assignment must have a completed cover sheet. It must be placed in the drop-box on or before the deadline. st SECTION A ELECTRONIC STRUCTURE & IONIZATION ENERGY 1. 2. Write the electronic structure in s‚p‚d notation of the following: O‚ Na‚ Na+‚ Al‚ Cl- and Co
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Determining the value of molar mass of chosen compound Marta Dürrigl 1mmA Chemical reaction: b.) CaCO3 (s) + 2HCl (aq) ----------- CaCl2 (aq) + CO2 (g) + H2O(l) AIM: The aim of this investigation is to experimentally determine the molar mass of carbon dioxide (CO2) by measuring its volume and calculating its mass. CO2 which we will measure will arise as a product of a chemical reaction between Calcium Carbonate (CaCO3) and Hydrochloric acid (HCl) as it is shown in the chemical
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