incubation. Β-galactosidase activity was estimated in lysed cells by measuring the rate of appearance of o-nitrophenolate (oNP) in an assay containing 2.7 mM o-nitrophenol galactose (oNPG) as substrate. The reaction was stopped by the addition of 0.4 M Na2CO3 and vortexed. The absorbance of the tubes were taken at 420nm and 550nm (ε=4.5 mM-1 cm-1) using the latter reading to correct for
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Survey of the Sciences Unit 4: The Science of Fireworks 7-7-14/7-14-14 What is a firework? An explosive or combustible used for display. In the 19th century the introduction of new ingredients such as magnesium and aluminum greatly heightened the brilliance of such displays. There are two main classes of fireworks‚ force-and-spark and flame. In force and spark compositions‚ potassium nitrate‚ sulfur‚ and finely ground charcoal are used‚ with additional ingredients that produce various types
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Investigating the Determining Characteristics of Cations and Anions Chem 111 Sec 560 Introduction: The purpose of this lab was to study the specific characteristics of cations and anions‚ and ultimately to be able to identify an unknown substance based on our studies and tests using the logic trees developed through the experiment. A logic tree is a graphical display of the findings from this lab which‚ through a series of yes/no questions‚ elimination
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Emilia Monroe August 27‚ 2014 Experiment #01 The Determination of the Percent of Water in a Compound CHEM 1315-022 For experiment one‚ The Determination of the Percent of Water in a Compound‚ the sole purpose of conducting this experiment was to determine the percent of water found in compounds such as Magnesium Sulfate‚ Copper Sulfate‚ and so on. Along with determining the percentages of the hygroscopic compounds the experiment allowed for the exploration of separation of hydrogen bonds
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Conductivity of Ionic and Molecular Aqueous Solutions Abstract Aqueous solutions that have free ions have higher conductivity values than solutions with most molecules. The purpose of the experiment was to see if ionic strength of an aqueous solution determines its conductivity. We tested each compound by putting the conductivity probe into its aqueous solution. Then the computer would analyze the data and give us a conductivity value. After each solution was tested‚ we used deionized water
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DKFLSDFKLFKJ | Acid-Base Titration. | kfjhdkjhvdkfj | | April Jowers | 12/19/2012 | DKFJSDKJFHDSKJHF | Introduction In this lab we will use basic titrating skills and techniques in order to titrate HCl. We will also be practicing how to prepare the solution. Using the titration data‚ we can practice our stoichiometric skills and also become more familiar with using lab equipment. Titration is the process of measuring the exact volume of a solution of known concentration that is
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Volume of Ca(OH)2 used 17.75mL 7.25mL ** average volume from two trials is 21.125mL Questions 1. Calculation of [OH-] from titration data (using average of all trials) C=0.0500M V=0.01L n= CxV n= (0.0500M)(0.01L) n= 0.0005mol 2HCl(aq) + Ca(OH)2(aq) CaCl2(s) + H2O(l) 0.0005 mol of HCl X 1 mol of Ca(OH)2 = 0.00025 mol of Ca(OH)2 2 mol of HCl Therefore‚ nCa(OH)2 = 0.00025 mol Ca(OH)2(aq) Ca2+ + 2OH- 0.00025mol Ca(OH)2 X 2 mol OH- = 0.0005 mol
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Observations: Part I: Reaction between iron and oxygen Appearance of steel wool after 20 minutes From (relatively) shiny metal strips to not-so-shiny strips that are stained with dark‚ reddish-brown stuff. Part II: Decomposition of hydrogen peroxide Appearance of potato and hydrogen peroxide combination: Lots of white‚ foamy bubbles around the potato slices. Day 2 Observations: Part III: Reaction between zinc and acetic acid Appearance of penny in vinegar after
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MODULE 2 WORKSHEET 6 CALCULATIONS INVOLVING GASES Syllabus reference 9.3.2 1 Two identical gas flasks‚ A and B‚ are kept at the same temperature and pressure. Flask A contains 10 g of ethane gas‚ C2H6. Flask B contains sulfur dioxide gas‚ SO2. Calculate the mass of sulfur dioxide gas in flask B. moles ethane 10/30 0.33 mol moles SO2 0.33 mol mass SO2 0.33 64 21 g 2 What mass of nitric oxide‚ NO‚ is present in a 2.5 L flask at a pressure of 100 kPa and 0ºC? mole NO 2.5/22.71
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CBSE Continuous and Comprehensive Evaluation (CCE) Sample Question Papers Solutions Term 1 (April to September 2013) Science Class OSWAAL BOOKS CONTENTS l Solutions q Sample Question Paper 6 q Sample Question Paper 7 q Sample Question Paper 8 q Sample Question Paper 9 q Sample Question Paper 10 1 - 7 8 - 13 14 - 20 21 - 26 27 - 32 ll SAMPLE QUESTION PAPER–6 Section-A 1. Moving charges / electric current / magnet (any two). 2. (i) To develop male secondary sex organs
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