Transformation of E. coli with your unknown plasmid Learning Goals: Insert your uncut unknown plasmid into chemically competent DH-5 E.coli cells and use antibiotic resistance to confirm the success of the transformation. You should familiarize yourself with the various methods of transformation and the advantages/disadvantages of each type. You should also understand how heat shock transformation works and how chemically competent cells make this type of transformation possible. For this transformation
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Introduction The purpose of this lab was to demonstrate the use of gene therapy on diseases that are caused by a single gene defect. This procedure was demonstrated on two different strains of baker’s yeast‚ EAY 235 and EAY 431‚ which both contained mutations in the LEU2 and TRP1 genes. Neither of these strains will grow without a proper medium that would supply both of these essential amino acids. The EAY 431 strain of yeast also contained a Rad 52 deletion‚ which caused EAY 431 to be a deficient
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Altered Nutrition 1. Nursing Process The nurse’s assessment findings include right sided weakness‚ slurred speech‚ and dysphagia. The nurse identifies that Mrs. Rusk is at high risk for several problems. 2. 1. In developing the nursing plan of care‚ which problem has the highest priority? A. Correct Aspiration. Aspiration‚ or the entry of foreign substances such as food or fluids into the lungs‚ may cause hypoxia or respiratory distress. Therefore‚ this is the highest priority in establishing
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Eosinophilic Esophagitis (EoE) the New Kid in Town Something Brand New! What comes to mind when you see EE or EoE? If you do not know what this stand for you’re not alone‚ only a handful of people know what those letters stand for. For those who do know what these letters stand for it is not a pleasant experience by any means. EE or EoE stands for eosinophilic esophagitis. What is this mouthful of a phrase you ask? EoE is an auto immune disease and is characterized as a relatively new disease
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Postoperative Diagnosis: Same. Operative Procedure: Right chest tube thoracostomy‚ pleura drainage. Anesthesia: Xylocaine 1%. Specimen Removed: Pleural fluid sent for cytology. INDICATIONS: This 61-year-old white male presents with recurrent pneumothorax on the right and increasing subcutaneous emphysema‚ despite earlier insertion of two small caliber chest tubes. Also‚ interval change in position of the right lower chest tube catheter has been noted radiographically. DESCRIPTION OF PROCEDURE:
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administered through an IV as well‚ but is seldom required. While a nasogastric (NG) tube can provide feedings and medications while reducing the risk of aspiration‚ it does not eliminate the risk of aspiration completely. The same is true for a percutaneous endoscopic gastrostomy (PEG) tube‚ which administers nutrients directly through the abdomen and into the stomach. Complications with these tubes may arise‚ such as premature removal of the tube or “osmotic gradients that lead to diarrhea” (Jauch et al
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Journal Article Review 1/2 The second article that I chose for this week was relating to the most common population that is seen in the pediatric developmental clinic: premature infants. The article begins with an introduction informing the reader of some statistical information relating to premature births and the costs of hospital stays. In the introduction‚ the problem is presented‚ informing the reader that there is a need for interventions that are evidenced-based‚ to help reduce hospital
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Intensive Care Units (ICUs) have undergone an impressive improvement and expansion over the last 50 years. Evidence strongly supports that the presence of a specialized critical care team which is directed by a dedicated intensivist physician as a leader reduce patients’ mortality and morbidity.1‚2 High quality care to critically ill patients also requires the adoption of a 24/7 intensivist model of critical care and the appropriate training‚ credentialing‚ and dedicating to critical care of all
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Amanda Lorenzetti Dr. Albert Farr Enc1101 5 October 2012 How to insert a Foley Catheter Catheter insertion is a medical procedure that empties the bladder of urine. First off‚ I should mention that there are three different types of catheters. The most common and the one I will be explaining in this paper is the Foley catheter or indwelling catheter. Which is a flexible latex tube inserted through the urethra and into the bladder. The tubing can also be made of Teflon‚ non-latex and silicone
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transformation. Remember that a gene is a piece of DNA which provides the instructions for making (codes for) a protein. This protein gives an organism a particular trait. Genetic transformation literally means change caused by genes‚ and involves the insertion of a gene into an organism in order to change the organism’s trait. Genetic transformation is used in many areas of biotechnology. In agriculture‚ genes coding for traits such as frost‚ pest‚ or spoilage resistance can be genetically transformed
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