2. Steps for Southern Blotting Digest the DNA with an appropriate restriction enzyme. Run the digest on an agarose gel. Denature the DNA‚ which would separate double-stranded DNA into single-stranded DNA. Transfer the denatured DNA to the membrane. Probe the membrane. Visualize your radioactively labeled target sequence. If you used a radiolabeled 32P probe‚ then you would visualize by autoradiograph. 3. a) She placed the nucleotide as she did because the EcoR I cuts in one
Premium DNA Molecular biology Gene
Earth Systems Quarter 3 Credit Recovery Overview Reference textbook: Glencoe Earth Science (2008) + Ecology Module (2005 or 009) Pre-Assessment 3 English versions 1 Spanish version Answer keys Modules 12 Assignments 3 Labs 1 Answer Key document that includes all assignments and labs Final Assessment 3 English versions Answer keys Materials List For lab work. Most materials are re-useable‚ but there are a few consumable items that will need replacing. newspapers or old magazines‚ scissors‚
Premium Project management Washington Ecosystem
Associate Program Material Appendix D Selecting a Topic and Brainstorming Worksheet Complete the following and post as an attachment. List two objects‚ people‚ subjects‚ or concepts you are going to compare and/or contrast. Mcdonald’s and Hardee’s What are the similarities between the two objects‚ people‚ subjects‚ or concepts? List as many similarities as you can. Both are fast food restaraunts and they both sell breakfast as well as hamburgers and they both have different
Premium Writing process Writing Learning
University of Phoenix Material Appendix D Part I Define the following terms: Term Definition Ethnic group Being a member of an ethnic group‚ especially of a group that is a minority within a larger society. Anti-Semitism Discrimination against prejudice or hostility towards Jews. Islamophobia A irrational fear or prejudice towards Muslims and Islam. Xenophobia A fear of foreigners or strangers or of that which is foreign or strange. Persecution The systematic mistreatment
Premium Roman Catholic Church Catholic Church Discrimination
Understand How to Organise and Report Data That Has Been Researched. 1.1 Describe different ways of organising data that has been researched. Data is a group of information that are used for various purposes like analysis‚ evaluations and to arrive at certain results or conclusions. Data reporting is a process where data is extracted form a source or many sources and then converted into a format that can be used for a purpose. It is usually presented in the form of a report in tables‚ charts‚ statistics
Premium Data Data analysis Chart
During the time of my career working as an LPN‚ I decided to work on a Sub-Acute unit in a nursing home. The unit consisted of 30 beds and each nurse assigned 15 patients. One night during the evening shift‚ I noticed the other nurse administering medications in the hallway to a patient in a wheelchair. I approached the nurse and questioned if it is appropriate to dispense medications in the hallway related to the policy of the company. I further elaborated on during orientation‚ the preceptor
Premium Patient Nursing Nurse
Pontificia Universidad Javeriana Facultad de Comunicación y Lenguaje Lingüística Aplicada Juliana Castañeda González Quang‚ V. D. (2007). Critical Applied Linguistics: Concerns and Domains. In this article‚ Vo Dai Quang’s objective is to understand what is Critical Applied Linguistics (CAL) and to do so‚ he lists some concerns and questions that can bring a closer understanding of what critical applied linguistics means. Quang’s concerns have to do with: “the scope and coverage of applied
Premium Linguistics Applied linguistics
Associate Program Material Appendix D Selecting a Topic and Brainstorming Worksheet Complete the following and post as an attachment. |List two objects‚ people‚ subjects‚ or concepts you are going to | | |compare and/or contrast. | | | |Hamburgers‚French fries
Free Writing Writing process Essay
What training solution was adopted by OCS? To unlock the potential of the OCS team and help them perform to their fullest abilities‚ the business embarked on the NVQ Level 2 Customer Service Programme. This NVQ framework gave a wide range of units relevant to job roles. Different achievement levels allowed easy progression and flexibility to undertake additional modules whilst ‘naturally-occurring’ service situations were used as evidence of competence. Has the training and qualification
Premium Customer service Skill Customer
Week 5 iLab Report Angelo Scianni DeVry University NETW360: Wireless Technologies and Services Observing the Wireless Spectrum Submitted to: Professor: Yassin Date: 8/5/14 Observing the Wireless Spectrum Introduction Guidelines: It’s always best to introduce a paper to the reader. It sets the tone and provides an overview of what will be covered and what the goals are. What is the intent of the lab? What issues are addressed? Why is it important? What are the goals? In this lab
Premium Wireless Radio Wireless network