In the first part of this lab‚ E.coli cells were transformed with an R-plasmid carrying a tetracycline resistant gene‚ giving rise to tetracycline resistant E.coli strain. This was accomplished through transformation‚ which allowed E.coli to directly uptake the naked DNA molecule carrying the antibiotic resistant gene (1). However‚ in order to take up the DNA and incorporate them into their genome via recombination‚ cells must be competent (1). Therefore‚ E.coli cells which are not competent under
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11 – 2013 Collegium Civitas TAP MA Course First‚ Some questions... How big is the EU budget? What are its sources? Which states do you think contribute the most to it? And which states are net-recipients? What is the common budget spent on? Is it larger than a state’s budget? How does it compare? Why has the budget and its negotiation become so controversial? Current budget negotiations highlight complex nature of the EU budget For first time ever its possible that the budget for 2014-2020
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The Happiness Advantage: The Seven Principles of Positive Psychology that Fuel Success at Work Traditionally‚ most people believe that in order to be successful they must work hard‚ and once they are successful‚ they will be happy. Today we have the beliefs that if we can just find that great job or win that promotion‚ happiness will follow. This theory may be the cause of many people leading unhappy lives. The Happiness Advantage: the Seven Principles of Positive Psychology to Fuel Success
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those in the writings of William Faulkner and Gabriel Garca Marquez‚ at the same time finding a departure point in old Chinese literature and in oral tradition‚" according to the citation for the award. Mo‚ 57‚ whose real name is Guan Moye‚ is the first Chinese writer to win the honor‚ which also comes with a financial award of 8 million Swedish krona‚ or $1.2 million. "I grew up in an environment immersed with folk culture‚ which inevitably come into my novels when I pick up a pen to write. This
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Advantages and dis advantages of credit cards More and more people are using credit cards now a days .As the number of people using credits cards rises‚ also the number of people with bad credit rises .Instead of carrying a wad of cash in your wallet it’s not really hard to think about carrying something small and convenient .How about a small piece of plastic that’s about four inches long. More people are using plastic currency more than paper. But before you think about of just getting just any
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The Advantages of Computer Technology in Second Language Accession Inspired by the rapid development of technology from the 1980s‚ computer has now become an influential component of second language learning pedagogy. Educators recognize that utilizing computer technology and its attached language learning programs can be convenient to create both independent and collaborative learning environments and provide students with language experiences as they move through the various stages of second language
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WORLD TRADE ORGANIZATION(WTO) Introduction: WTO is an international body founded in 1995 to encourage international trade and economic development by reducing tariffs and other restrictions.This organization deals with regulation of trade between participating countries; it provides an outline for negotiating and formalizing trade agreements‚ and a dispute resolution process. WTO’s current Director-General is Pascal Lamy‚ who leads a staff of over 600 people in Geneva‚ Switzerland. Agreements:
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The Advantages of Drones By: Jacob Griffin INTRODUCTION: Since the beginning of time‚ man has had a great desire to fly. In 1903‚ the Wright Brothers were the first men to achieve sustained‚ powered flight. Long after man first flew and with the invention of advanced electronics‚ people decided they wanted to fly small planes for themselves as a hobby. For years‚ people have enjoyed flying radio controlled aircraft. An advance in technology correlates to an advance in electronics‚ which results
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World Trade Organization and Regional Trade Agreement do they interfere in each other business. Introduction: World trade organization is an international organization that regulate trade between nations. The purpose behind it is to help procedure of good and services‚ exports and imports conduct their businesses (What is the WTO?‚ 2014). Based in Geneva Switzerland World Trade Organization which was established in 1 January 1995‚ Created by Uruguay Round negotiations‚ there are 160 member countries
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