(ELECTROPHORESIS OF SERUM PROTEIN) DATE : 10 OCTOBER 2013 PART A SEPARATION OF SERUM PROTEINS USING THE ELECTROPHORESIS METHOD. OBJECTIVES: 1. To understand the analytical methods involved in analyzing serum proteins. 2. To study the serum protein electrophoresis pattern to aid the understanding of the relationship between the structure and the function of proteins. PRINCIPLE OF PRACTICAL: This technique is based on the movement of charged particles such as proteins when placed
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If pH > pI‚ then the protein will have a negative charge and if pH < pI‚ the protein will have a positive charge. Buffer I has a pH >5‚ meaning both proteins carry a negative charge and bind to the DEAE (a positively charged resin). (b) pH = pKa + log10(Base/Acid) [Base = mM of sodium acetate; Acid = mM of acetic acid] = 4.7 + log10 (40/40) = 4.7 In order for the catalase to elute from the column‚ it must have lost its negative charge and stopped binding to the DEAE. Lowering the pH
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Title: Purification of Green Fluorescent Protein Introduction: Transformation is used to introduce a gene coding for a foreign protein into bacteria. Hydrophobic Interaction Chromatography (HIC) is used to purify the foreign protein. Protein gel electrophoresis is used to check and analyze the pure protein. Research scientists use Green Fluorescent Protein (GFP) as a master or tag to learn about the biology of individual cells and multicultural organisms. This lab introduces a rapid method
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In the past protein powder has been the main product of choice of bodybuilders and other weightlifters who spend all of their time in the gym with the goal of becoming as strong and muscular as they can. But‚ in the past years‚ many other athletes and people have discovered the many benefits that protein powders have‚ causing the popularity of this product to increase rapidly. Many studies have proven that using protein powder while performing a resistance exercise plan will increase overall muscular
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Background: Pepsinogen is an inactive protein that is the mucus lining of the stomach. When converted to pepsin‚ the enzyme is used to break down large or undigested protein they has been absorbed by the small intestine. The digestive power of pepsin is greatest at the acidity of normal gastric juice (pH 1.5–2.5). In the intestine the gastric acids are neutralized (pH 7)‚ and pepsin is no longer effective. "Pepsin (biochemistry)." Encyclopedia Britannica Online. Encyclopedia Britannica‚ n.d. Web
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Objective: To investigate how pH affects the rate of protein digestion Hypothesis: I predict that since pepsin breaks down the material the quickest at a pH of 2 in the stomach‚ test tubes #3 will reduce the greatest because it has pepsin to further break down the egg. Whereas the other test tubes will have a smaller reduction. I think test tube #2 will have the second largest reduction rate and then #6 because they all will have a greater rate at breaking down the material‚ Then‚ I predict the
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It makes sense to think that quick‚ post-workout protein intake might boost recovery and promote higher performances on subsequent days of training. Indeed‚ some research has suggested that protein intake can help athletes adapt to their workouts more effectively. However‚ such studies have been flawed methodologically‚ and a new investigation reveals that combining protein with carbohydrate after workouts is not a superior strategy‚ compared with taking carbs alone. You’ve heard them: The "experts"
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G-protein Coupled Receptors (GPCRs) are cell surface proteins that relay extracellular signals to the nucleus. These signals can be in the form of light‚ peptides‚ lipids‚ and sugars. Once activated by a ligand at the outer cell surface‚ GPCRs activate their cognate G-proteins that reside on the inner cell membrane. The activated G-proteins then slide along the inside of the cell membrane to activate a cascade of proteins and enzymes that in turn amplify the original message received by the GPCR
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Protein powder is bad because it contained a lot of additive‚ sugar and too much protein. Although natural protein provide immune system defense for the body and also contain antioxidant components‚ however‚ that does not mean that protein shake or protein powder has the same function as the natural protein. As time goes by‚ protein powder has become essential for muscle builder to consume. People drink protein shakes or take pills while they are working out or after performing intense exercise.
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The first half of module seven discussed protein digestion‚ absorption‚ and metabolism; while the second half focused on pathological stress and kidney disease. Protein digestion occurs in the stomach and small intestine. When a protein reaches the stomach it is denatured by hydrochloric acid. Once the protein is digested pepsin breaks apart the denatured protein into peptides. Peptides move into the small intestine where they encounter an enzyme that digest the peptides into di- or tripeptides‚
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