Gram Stain: Gram is an empirical method to distinguish the species of bacteria into two groups Gram-positive and Gram-negative based on the physicochemical properties of the cells. First‚ a smear was prepared by use a sterile transfer loop that been flamed to removes some bacteria from slant agar and placed on the slide; mixed with one drop of water and let air dried. After dried‚ heat fixation the slide by passed the slide over a flame quickly 2-3 times to stick bacteria to the slide. Next‚ the
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Acid and Base Titrations: Preparing Standardized Solutions Introduction: This experiment focuses on titrations of acids and bases. A titration depends on addition of a known volume of solution and is a type of volumetric analysis. Many titrations involve either acid-base reactions or oxidation-reduction reactions. In this experiment we do one of each. We monitor the pH of the reaction with the use of a color indicator. We also learn about the standardization of bases (NaOH) and acids (HCl) which
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the pH titration curves for the titration of a strong acid with a strong base and of a weak acid with a strong base‚ and find Ka of the weak acid. Procedure: Part I 1. Pour 30 ml of the HCl solution of unknown concentration in a 100 ml beaker. 2. Add a stirring magnet. 3. Place the beaker on the magnetic heater and activate the rotating motor but not the heater. 4. Insert the probes of the calibrated pH-probe in the beaker and record the pH. 5. Position a burette over the beaker and fill it up with
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Chapter 4: Nucleic Acids and the RNA World 1. 4.1 – What is a Nucleic Acid? * Nucleic acids are made up of monomers called nucleotides * Three components of a nucleotide: 1. Phosphate group—attached to the 5’ carbon 2. Sugar – carbonyl group and several hydroxyl groups 3. Nitrogenous base * The prime (‘) symbols indicate the carbon being is part of the sugar—not attached to the nitrogenous base. * Four different nucleotides‚ each of which contains a different nitrogenous
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ICSE Class X Chapter 5 Hydrogen Chloride and Hydrochloric Acid Hydrogen chloride (HCl) : Formula : HCl Nature : Covalent. Molecular Mass : 36·5u [i.e. HCl = 1 + 35·5 = 36·5] Occurrence : (i) Present in gastric juices. (ii) Present in volcanic gases. Laboratory preparation of HCl gas : Hydrogen chloride gas is prepared in laboratory by heating conc. H2SO4 with NaCl. NaCl + H2SO4 Heat → NaCl + NaHSO4 Heat → NaHSO4 + HCl Sodium hydrogen sulphate Na2SO4 + HCl
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Acid-Base Extraction: Separation of an Organic Acid‚ Base and a Neural Compound Introduction/Background Acid base extraction is a widely used commercial method in separating acids‚ bases and neutral compounds. This is concept is based on several physical properties of the compounds‚ such as boiling point‚ melting points and solubility. The different solubility properties of the protonated and non-protonated forms of these compounds depend on the functional groups present‚ such
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Cause and Effect on Acid Rain Introduction Beautifully crafted statues are now worn out or severely damaged‚ aquatic wildlife population are dying off‚ forests have lost the leaves that protect their trees and produce food‚ and the rates of lung diseases in humans are rising around the world. The cause of all of these problems can be linked back to acid rain. Most people don’t understand the concept or science behind acid rain. The common belief is that it’s a natural process‚ but it’s actually
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solution or a known quantity of solid dissolved in solution. It may also be used to calculate the molar mass of an unknown acid or base. Purpose: I. Prepare a standard solution of sodium hydroxide II. Standardize a sodium hydroxide solution by using potassium hydrogen phthalate (KHP) III. When given whether the acid is monoprotic‚ diprotic‚ or triprotic ‚ determine the molar mass of the unknown organic acid. IV. Calculate the Molarity of the Soft drink. Materials: * 50 mL
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Abstract Acetic Anhydride and p-Aminophenol were heated in a vial attached to an air condenser to synthesize crude acetaminophen‚ resulting in 0.097 grams (47.48% yield). The crude acetaminophen was then recrystallized in a solvent of water and methanol over heat resulting in 0.082 grams (39.61% yield) of pure acetaminophen. Melting points of both crude and pure acetaminophen were taken‚ and found to be 165.9 - 170.9°C and 168.2 - 171.5°C‚ respectively. The literature melting point of acetaminophen
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Jennifer Everett Phase 4 DB Instructor: Clement Yedjou 3/10/2015 Acid rain Acid rain is a result of air pollution that is harmful to the environment‚ generally caused by fuels being burnt. When any type of fuel is burnt‚ many different chemicals are produced. These gases that are released react with the water in clouds‚ and the rain from these clouds is acid rain. This type of acid disposition can appear in many other forms besides just rain‚ such as sleet‚ snow‚ and fog (Editorial Board‚ 2013)
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