determine the protein concentration of the solution from the standard curve by diluting the solution. Dilution itself is a process of lessening a solute’s concentration in a solution. In this experiment‚ dilution can be done by reducing the amount of protein solution from 1.0 ml to 0.2 ml. Without the process of dilution‚ the absorbance will end up being an extrapolated value causing the result to be unreliable. Part 2 6.
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x 30cm) String for tying dialysis tubing Clear plastic drinking cups Sharpie Markers Funnels Glucose/starch solution Distilled water IKI solution Glucose TesTape 0‚ .2‚ .4‚ .6‚ .8‚ and 1 Molar Sucrose Solution Cork Borer Potatoes Scale Exercise 1: Diffusion Fill a dialysis bag with a sugar/starch solution and immerse the bag in a dilute iodine solution. Water‚ sugar‚ starch‚ and iodine molecules will all be in motion‚ and each molecule will move to a region of its lower
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solvent. The higher the solute in solvent‚ then there will be a higher rate of osmosis through the diffusion gradient forming a hypertonic or hypotonic solution. Solvent with equal or no solute forms an isotonic solution. Materials: Distilled water‚ sucrose‚ dialysis tubing‚ string‚ 250 ml beaker. Procedure: To demonstrate and isotonic solution we needed 3 inches of dialysis tubing. We tied off one end of the dialysis tubing to create a bag‚ filled it with distilled water‚ and tie of other end
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Introduction: To determine the biological changes that occur to potato cores over a period of time in different solutions of sucrose and to relate these changes to the phenomenon of osmosis. Method: We soaked several discs of potato cuted using a cork borer with around 1 centimeter of diameter and 2 milimeters of lenght into sucrose solutions with a different range of concentrations from 0 to 1.0M. Then we weighed all the potato cylinders on an electronic balance
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Lab Report Purpose: Osmosis and diffusion through potato core in different concentration (viable) : The concentration of solution in a solution will affect the movement of water across potato cell membrane Material: Potato core Sucrose solution (0.2‚0.4‚0.6‚0.8‚1.0) Electronic Balane Test tube and rack Scalpel Plastic Weighing tray Cork Borers Method: Day 1 use you cork borers to bore 6 vertical hole on your potato. remove the potato cylinders from
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experiment is to investigate the osmosis effect in potato cores while they are submerged in different concentrations of sucrose solution. Osmosis will take place while the potatoes are fully submerged in the sucrose solution. Apparatus æ Scalpel (1) æ Tile (1) æ Pr. Tweezers (1) æ 250ml Beakers (2) æ 100ml Water (Per Beaker) æ 100ml Sucrose Solution o 0.2M o 0.4M o 0.6M o 0.8M o 1.0M æ Measuring Cylinders (2) æ Ruler (1) æ Potato Cores (30) æ Stop Clock
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The process of osmosis doesn’t stop till both solutions are equal in and out of the cell membrane. Osmotic pressure is needed to stop the process of osmosis. “The rate in which osmosis occurs depends on the temperature of the solution‚ the concentration of the solute‚ and the electrical charge of the solute.” Osmosis occurs in the large and small intestines. After you chew your food it goes
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Abstract This report investigates the absorbance of methylene blue and carmine red using a spectrophotometer to determine the absorption spectra of both solutions. The concentration of the unknown solution of methylene blue was found to be 1.07 x 10-5 M by using the molar extinction coefficient‚ with absorption of 0.547. It was also found that the results concluded confirmed beer’s law with an R2 value of 0.9989. Introduction Spectrophotometry is the quantitative measurement of the absorbance
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Question 1: How does Osmosis works It refers to the movement of water molecules from an area of high-level water concentration‚ that is‚ a dilute solution‚ to an area of low-level water concentration‚ that is‚ a strong solution through a semi-permeable membrane. (Passmyexams.co.uk‚ 2015) Actually‚ water moves in a bi-directional manner with the aim of leveling up the concentration. However‚ a high number of water molecules move from the fresh water towards the salty water. The outcome
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Lab # 4 Determination of Density of Liquids Name: Lab Partner: Period: 3 Date Completed: 9/23/2014 Date Submitted: 9/29/2014 Data TABLE 4 DENSITY OF SALT SOLUTIONS-INDIVIDUAL GROUP’S RESULTS Concentration (%) Mass (g) Volume (mL) Density (g/mL) 0 9.9522 10.00 0.9952 4 10.1291 10.00 1.013 8 10.5233 10.00 1.052 12 10.7487 10.00 1.075 16 11.0297 10.00 1.103 Unknown # 10.6234 10.00 1.062 Calculations 1. Show all density
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