liquid nutrient broth which supports the growth of a wide variety of microorganisms whilst the agar function is the solid media onto which the bacteria can be isolated as independent colonies which are representatives of different bacterial species. Controlling microbial growth is necessary in numerous situations and is greatly significant in areas such as medicine. Growth of microorganisms is
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Morinda citrifolia / Noni Morinda citrifolia is a tree in the coffee family‚ Rubiaceae. Its native range extends through Southeast Asia and Australasia‚ and the species is now cultivated throughout the tropics and widely naturalised. It is commonly known as great morinda‚ Indian mulberry‚ nunaakai (Tamil Nadu‚ India)‚ dog dumpling (Barbados)‚ mengkudu (Indonesia and Malaysia)‚ apatot (Philippines)‚ kumudu (Bali)‚ pace (Java)‚ beach mulberry‚ cheese fruit or noni (from Hawaiian). •
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IDENTIFYING GRAM POSITIVE COCCI As mentioned in Exercise 8‚ “Identifying Gram Negative Rods”‚ identifying bacteria is a common activity in the microbiology lab. Like the game Clue™‚ each time you gather a piece of information to solve the mystery‚ you gather some information that supports some identities and eliminates others from contention. In the lab‚ the process continues as you gather more information until only one microbe remains and all others have been eliminated as possibilities. Thus
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diet to ensure they receive the proper nutrients. Benefits of taking dietary supplements can be abundant if you take them correctly and consult your physician. This enables you to get all the benefits that dietary supplements are uses for. Dietary supplements can prevent disease‚ promotes a good metabolism‚ and can aid in tissue and muscle restoration. The biggest benefit of a dietary supplement is to ensure you are receiving the correct amount of nutrients by taking a supplement that you are
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moved from one organism to another with the use of pGlo plasmids. It was hypothesized that if bacteria that were transformed with +pGlo plasmids are given the gene for GFP‚ then transformed cell colonies will be located on the LB/amp/ara and LB/amp agar plates. Cells that have been transformed with +pGlo plasmids have the ability to grow in ampicillin plates‚ and the arabinose sugar allows the colonies to be visibly fluorescent under ultraviolet light. The GFP is able to resist ampicillin because
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Nutrient Deficiency Four classes of essential nutrients 1. Amino acids 2. Fatty acids 3. Vitamins 4. Minerals (Obtained by ingesting and digesting them) Provide continuous energy for ATP production: cellular respiration and storage Used to build complex molecules for: 1. Growth 2. Maintenance 3. Reproduciton Amino Acids and Protein Deficiency 20 amino acids are used to make proteins Synthesize about ½ using organic N Essential are the remaining Average
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plate and the streak plate inoculation procedure for the separation of the cells of a mixed culture so that discrete colonies can be isolated. ii. To prepare a stock culture of an organism using isolates from the mixed cultures prepared on the agar streak-plate and/or the spread plate. Introduction : In order to be able to adequately study and characterize a certain microorganism‚ microbiologists need to separate and isolate this microorganism from the many other microorganisms with which
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antiseptics on bacterial growth. By observing the effects of 5 different inhibitors including alcohol‚ bleach‚ soap solution and distilled water‚ it was determined what antiseptic or disinfectant was able to best inhibit this kind of bacterial growth. Nutrient Agar was poured into a Petri dish with four quadrants and then a pipette was used to place bacterial culture on top. Using forceps‚ a filter disc was dipped into each inhibitor and then into a separate quadrant of the Petri dish. The lid of the Petri
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molds‚ since they are readily carried about by air currents. Septa = hyphal cross walls which divide the filaments into separate cells. Petri Plate (dish) = a special covered dish in which mold is cultured. Medium = a solid nutrient used for culturing. Agar = a non-nutrient thickening agent which is thicker than gelatin but still quite soft. Smear = a thin film of microbial cells on a microscope slide. Fixing = passing the smear through the flame of the laboratory burner three times in rapid
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were made and recorded each week to narrow down the scope of identification. Data has been presented in the tables‚ charts and drawings herein and reflect the results of microscopic observations as well as the differential tests results on various agars and broth cultures. Although all tests were not conclusive‚ the unknown organism labeled Unknown #11 was found to be a member of the family Enterobacteriacea and Genus Serratia marcescens. INTRODUCTION The field of Microbiology is the
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