This experiment tested the growth of E.coli with inserted plasmid on an agar plate with Ampicillin. One colony of E.coli resistant to Ampicillin was grown during this experiment. The overall goal of the experiment was to successfully grow E.coli on the agar plate‚ which would show that the plasmid had been effectively inserted into the bacteria’s genes. This experiment helped students understand how plasmids were inserted into bacteria and used in real life situations. It also showed how the bacteria’s
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Lab 11 Methodology By using aseptic‚ a little cultured bacteria was inoculated on the TSA agar. A quadric streak was making. Inoculation loop was heated and keep it cold for a while before the next quadratic streak. Six agar plates were observed for 24 hour at temperature of 30ºC. Choose one from the dense colony and make a sub-culture on the new agar plate. The step was repeated to get a single colony‚ which is pure colony. a) Sequestration of bacteria from fish organs Methodology
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unknown mixture‚ using a number of laboratory tests. Over the semester‚ multiple tests were carried out to identify between gram-positive and gram-negative bacteria including the Mannitol Salt Agar (MSA) media test‚ which selects only for gram positive bacteria‚ and the use of Eosin Methylene Blue Levine (EMB) Agar media‚ which selects for gram negative bacteria and differentiates between lactose fermenters (paracolons) and non-lactose fermenters (coliforms).These tests along with other selective and/or
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vegetables daily Taking megadoses of various vitamin supplements daily Eating 4 ounces of chicken liver once a week None of these are correct. 4.what is the proper diet for a cow? Grass 5. Which of the following substances is a nutrient that does not provide any energy for the human body? Water Protein Carbohydrate Alcohol 6. Scientists conduct a study in which 100 adults with chronic diarrhea are divided into 2 groups of 50 people. One group is given a
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HEALTH FOOD DRINKS? Health food drinks are nutrient supplying drinks which are prepared by adding malt or wheat based powders to milk. There are some health food drinks which are prepared from other ingredients unlike malt or wheat. All the powders contain vitamins and minerals like B1‚B2‚B6‚B12‚ vitamin D‚ calcium‚ iron‚ copper‚ niacin‚ magnesium etc which will help in building the body by acquiring energy . Different kinds of Health Food Drinks are available in the market with different
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capsules‚ or pill forms. They add to the diet and should not be substituted for food that we eat. What are some benefits of taking dietary supplements? Dietary supplements can or may help a person obtain an adequate dietary intake of essential nutrients needed for the human body. As I stated before‚ dietary supplements are not a substitute for food‚ therefore‚ it is always necessary to eat a variety of nutritious
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facilitates the handling of large numbers of bacterial clones for classification on a variety of media. METHODS Replica plating. A frequent chore in bacteriological work is the transfer of isolates from one substrate to other selective or indicator agar media. In place of an inoculating needle‚ one might imagine a device consisting of many needle tips in fixed array‚ so that one operation would substitute for repeated transfers with a single needle. The requirements of this design are met by pile
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and starch agar. OBJECTIVES: distinguish between different bacterial species based on colony morphology on agar plates To distinguish the growth characteristics of microorganisms in various differential‚ and selective media. Differentiate bacteria based on their ability to hydrolyze starch. Materials: Plates of EMB‚ Starch and blood agar. Stool sample. Inoculating loop. Bunsen burner. Soil sample. Cotton soap. Skin sample. Gram iodine. Results: Starch agar: Special
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Purpose The principle of this experiment is to have a thorough perceptive of: • Culture washed and unwashed lettuce on agar plate. • Culture fresh and opened milk with the same expiration dates. • Explain the significance of food safety. • Illustrate foodborne sicknesses.
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that will be of use to you throughout your biological work. Procedure 1. Heat the test tubes of sterile agar medium in the water bath until the agar melts. 2. Remove the test tubes from the water bath. Let them cool enough to hold in your hand‚ but not so much that the agar becomes solid again. Perform the following transfer as quickly as possible. You must work rapidly so that the liquid agar will not cool and solidify before the transfer is completed. 3. Hold both a test tube
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