placed in agar plates simulating different environments: the bacteria lacking the pGLO plasmid was subjected to an environment solely contaiing nutrients and another containing nutrients and ampicillin; the bacteria containing pGLO was subjected to an environment containing solely nutrients and one containing nutrients‚ ampicillin‚ and the sugar arabinose. After being allowed to grow in their respective environments‚ the following agar plates grew E. Coli colonies: agar containing nutrients and a colony
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control plates. This indicates that the Tn10 exposure was the cause of mutant variation. There were ten hypothesized auxotrophic colonies based on the comparison of mutagenized colonies in minimal and nutrient agar. These ten colonies were confirmed to be auxotrophic by their lack of growth in the minimal agar replica plate. The required growth factors for each auxotroph were determined based on which pool plates fostered growth for each streak. Auxotrophs 1‚ 2‚ 3‚ and 4 require valine as a growth
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title for each of the two experiments you did : (i)‚ Experiment 1 demonstrated the growth of bacteria when placed in liquid nutrient broth culture‚ the number of species present had increased in growth. .(1) (ii) Experiment 2 illustrated the growth of bacteria when placed on different surfaces of solid agar plates which included: nutrient agar‚ CLED agar and MacConkey agar; the number of species present also had increased in growth. (1) 2(A). Experiment 1: Choose 2 words that describe the appearance
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Staphylococcus aureus‚ were grown singly and mixed on four different types of agar in order to observe the varying morphologies within the colonies. Resulting data was analyzed to provide understanding of the use of differing culture media and conditions for bacterial growth. RESULTSFour different agar types were used in this experiment. The first (Nutrient) allowed for growth of both E. coli and S. aureus. The second agar used (MKL) inhibited the growth of S. aureus but allowed the growth of E. coli
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3 antibiotic dicks | Distilled water | Culture of Serratia marcesans | Sterile filter-paper disks | Transparent tape | 1 sterile nutrient agar plate | Metric ruler | Forceps | 1 sterile cotton swab | Safety goggles | Lab apron | | Materials: Procedure: In the Antibiotic Resistant Bacteria lab‚ the effect of different antibiotics on the zone of inhibition on bacteria‚ Serratia marcesans‚ was measured in millimeters. The safety equipment‚ lab apron and goggles were worn at all times
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2010‚ p.157). Although microbes can be found everywhere around us‚ such as soil‚ water‚ food‚ sewage‚ body surfaces and also air‚ but to grow microbes is laboratory for research purpose‚ different microbes may have different growth requirement. A nutrient material prepared for the growth of microbes in a laboratory is known as the culture medium. Some bacteria can grow well on just about any culture medium while the other required special media‚ and still others cannot grow on any nonliving medium
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causative bacteria or fungus. Potato dextrose agar is a good nutrient agar for mycelia to thrive on which is present in most fungal moulds.1 Standard nutrient agar is a general utility used for non-fastidious microorganisms.2 Aim The aim is to isolate fungi and bacteria colonies from diseased and healthy leaves. Materials and Methods Materials used for the experiment was two of each: standard nutrient agar plate and potato dextrose agar plate. To remove any epiphytic or saprophytic
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Equipment: * 3 agar plates * Blank discs * Antibiotic assay disc * Culture of Bacillus subtilis * Forceps * Bunsen Burner * Inoculating loop * Tea Tree oil * Heat Mat Method: First Agar Plate * Sterilise inoculating loop using Bunsen burner‚ allow inoculating loop to cool before using inoculating loop to pick up‚ the liquid of the culture. * Agar plate to be open as little as possible‚ minimising
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semi-permeable outer membrane of E. coli would protect the bacteria from any antimicrobial properties of cilantro. A drop of cilantro juice and water in varying concentrations (1:10‚ 1:20‚ 1:40‚ 1:80) was added to a nutrient agar plate inoculated with S. epidermis and a nutrient agar plate inoculated with E. coli. The plates were incubated for 48 hours and then observed for a zone of clearing where the cilantro juice drop was placed. Cilantro was found to not display antimicrobial activity against
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Materials • 22 prepared nutrient agar in petri dishes • Timer • Starburst candy (11) • 22 sterile swab • ½ slice of Bologna- Oscar Myer (11) • Sterile gloves G. Procedures 1) Prepare 4 petri dishes with nutrient agar. 2) Put on your sterile gloves 3) Pick up your first food (Starburst candy) and drop it on the ground. 4) Start the timer. 5) Pick up the Starburst candy
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