volume ratio. As the surface area of the Agar cube increases‚ the rate of conductivity will steadily increase‚ too. The trend line of the graph shows as an exponential graph because at one point there will be no more conductivity due to too small or too big Agar cubes. A difference can be seen in the average rate of conductivity depending on surface area because the Agar cubes with a larger total surface area are able to diffuse the ions faster. When the Agar cube has larger surface area‚ more area
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dilution process in which culture is spread over an agar plate in a certain manner. Using a loop rod‚ culture was taken from the tube and dragged across area 1 several time‚of the agar. The agar was then turned 90º‚ and the loop was flamed and cooled. Taking some culture from area 1‚ it was dragged over area two‚and the same steps were done for areas 3 and 4.Another technique used was spread-plate‚ where the same culture is spread over the agar plate using a sterile L-shaped bent glass rod. The rod
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fight‚ and would stop its growth. HA- If we add E. coli and B. subtilis to agar‚ and add Penicillin and Tetracycline to the agar‚ then the E. coli will grow more around the Penicillin and the B. subtilis will grow more around the Tetracycline‚ because E. coli is resistant to Penicillin and B. subtilis is resistant to Tetracycline. HO- If we add E. coli and B. subtilis to agar‚ and add Penicillin and Tetracycline to the agar‚ then the E. coli will grow more around the Tetracycline and the B. subtilis
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types of media used to grow microbes. They consist of Enriched media‚ Nutrient media‚ Selective media‚ and differential media. Enriched media is a growth media supplemented with complex biological molecules. This type of media is used for blood‚ coagulated blood‚ amino acids‚ etc. Nutrient media provides nutrients necessary for the growth of microorganisms. Selective media is used for specific organisms. For i.e. Mackonkey agar only grows on gran(-). If a sample is negative it grows and if it is positive
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Experiment No. 03 Date: Name of the experiment: Aerobic plate count (APC) and total coliform count of supplied yogurt sample. Purpose: To determine the aerobic plate count and total coliform count of the supplied yogurt sample. Principle: Yogurt squeezed or extracted from milks are more or less acidic‚ depending on the product‚ the pH generally ranges from about 2.4-4.2 and contain sugar (4.7g)‚ fat
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diffusion rate in agar cubes? Hypothesis: The rate of diffusion in directly related to the surface area to volume ratio of cells and is responsible for the efficiency of absorbing nutrients‚ oxygen‚ minerals etc. in the cell. This ratio is specific to cells as they require a ratio that isn’t big enough to take too long to receive the nutrients and oxygen or too small to impair diffusion‚ known as the maximum sustainable cell size. Due to this it would be expected that the smaller the agar cube the larger
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The aseptically inoculate nutrient broth tubes A and B did not have any microbes inoculated into the tubes so it should not have been cloudy and that was what was seen. Tube C should have been cloudy because it had E. coli inoculated into the nutrient broth and that was also seen. A simple streak was also performed and it looks has if the loop went over previous streaks which should not have been done. A complex streak was also done. No colonies grew towards the end of the streak‚ this could be due
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Microbiology Module 02 Homework Assignment Use the information presented in this module along with additional outside research to answer the questions: 1) Compare and contrast prokaryotic and eukaryotic. a) Prokaryotes and eukaryotes are two types of cells that are very different but share some certain properties such as methods of reproduction‚ protein synthesis‚ an organized metabolism‚ response to stimuli‚ and plasma membranes. One significant difference is that prokaryotes are without a cell
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ISOLATION OF INDIVIDUAL BACTERIAL COLONIES ON SOLID MEDIA Robert Koch developed a method for isolating pure cultures on solid media in 1883. To this end he added agar (a solidifying agent) to liquid nutrient broth; the nutrient broth supports the growth of a wide variety of microorganisms while the agar provides a solid substrate on which bacteria can be mechanically diluted and therefore isolated as independent colonies representing different bacterial species. The isolation of independent bacterial
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Laguna. B. Maintenance of Microbial Cultures Bacterial isolated were subculture on slants of Nutrient Agar (NA). Incubation of the subcultured specimens for bacteria was done for 7-14 days in Isolation Room. C. Media Preparation for Assay Three tubes with nutrient agar were prepared. The cultures were inoculated onto their respective media by placing 0.2ml of each. The inoculated agar was then poured to individual sterile plates. D. Extraction from Leaves The extraction was done
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