should be opened into a 90o angle and the small end bent to close it. * 1 Sharpie marking pen. * 1 glass test tube with a cap containing 2 ml of sterile nutrient broth and labeled "Broth". * 2 Petri dishes containing only nutrient agar and labeled "No Amp" on the bottom with date. * 2 Petri dishes containing nutrient agar and the antibiotic ampicillin. The dishes should be labeled "Amp" on the bottom with the date. * The laboratory instructions. Methods: Pre-Lab:
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(RESEARCH TREATISE) By SITUMBEKO LIWELEYA (s213459531) Submitted in fulfilment of the requirements for the degree of BACHALOROUS TECHNOLOGIEA: BIOMEDICAL TECHNOLOGY At the At Nelson Mandela Metropolitan University Port Elizabeth‚ 2013. SUPERVISOR- PROFESSOR SMITH. N. DECLARATION I‚ the undersigned‚ hereby declare that the research work contained in this study is my own original work‚ and all the sources I have used or quoted have been indicated and acknowledged
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points Describe the Kirby Bauer Test? Make sure you describe all the key elements. 1. Using sterile technique‚ inoculate 3 nutrient agar plates individually with: a. E. coli b. S. aureus c. M. smegmatis 2. Place antibiotic disks evenly spaced on the inoculated agar plates and incubate at 37C for 24-48 hours. 3. Using sterile technique‚ inoculate 3 nutrient agar plates individually with: a. E. coli b. S. aureus c. M. smegmatis 4. Using sterile technique place disk in each of the solutions:
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LB/AMP/+PGLO‚ LB/AMP/-PGLO‚ LB/-PGLO. These threes represented nutrients and ampicillin‚ plasmids and arabinose. -The purpose to bacterial transformation is to insert DNA into bacteria to change it’s traits. Materials: Quantity: E. coli starter plate 1 Poured agar plates 4 Transformation solution 1 LB nutrient broth 1 Inoculation loops
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Question: What antibiotics work best in preventing E. coli k12 from growing; amoxicillin‚ ampicillin‚ or ciprofloxacin? Hypothesis: If ciprofloxacin‚ an antibiotic‚ is added to petri dishes covered in live bacteria and left to sit for four to six days in an incubator at 37 degrees Celsius‚ then the petri dishes containing ciprofloxacin will have the largest zone of inhibition out of all of the other antibiotics tested because research shows that ciprofloxacin in one of the strongest antibiotic currently
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for transformation to occur and then the solutions were placed on an agar plate and incubated at 37° for a week. Our results failed to reject our hypothesis and predictions and thus resulted that there was indeed a high percentage of transformation efficiency. The agar plate with the ampicillin environment but no plasmid uptake resulted in no growth while the rest that were a variation between ampicillin environment and nutrient broth have both lawn growth and colonial growth. Introduction
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testing will be done to determine the species of bacteria. It is predicted that Pseudomonas syringae will be the pathogenic bacteria. Materials and Methods: The bacteria from a rotten tomato was streaked on nutrient agar plate. The different colonies were then isolated and grown on nutrient agar slants. Slices of healthy tomato were inoculated using the grown bacteria (one type of bacteria per slice) and‚ there was a control which was not inoculated. The slices of tomatoes were allowed to incubate
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and human body samples by culturing them on the appropriate media using aseptic transfer techniques. Materials • Distilled water • Test tube • 6 Unopened packages of 1 sterile cotton swab • 2 sterile nutrient agar Petri dishes • 1 sterile blood agar Petri dish • Incubator • Refrigerator • Bunsen burner • Gas connection • Plastic tubing • Inoculating loop • 12 sterile glass slides • Wax pencil • Igniter • Crystal violet
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Gram-negative bacteria and fungal strains by measuring zone of inhibition. The antimicrobial activity was performed by Agar disc diffusion method at concentration level of 2.5‚ 5.0‚ 7.0‚ 10µg/ml respectively. Ampicillin (antibacterial)‚ Itraconazole (antifungal) as the standard drug at a concentration of 200µg/ml. LB Agar was used as the culture media for antibacterial and potassium dextrose agar was used as culture media for the antifungal activity. The results of the antimicrobial activity are shown in
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General Purpose media is designed to grow most organisms and do not contain growth inhibitors. Standard Methods Agar and Blood Agar Bases are examples of general purpose media. Differential Media Differential media contain a component that allow an observable change when a specific chemical reaction takes place. Simmons Citrate Agar is an example of a differential medium. In Simmons Citrate Agar‚ there is a pH indicator that turns from green to blue when citrate is utilized as the sole carbon source
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