Streak Plate Isolation for Obtaining Pure Cultures Data Interpretation 1- In order to unsure that you get a pure sample. 2-pure culture: laboratory culture containing a single species of organism. A pure culture is usually derived from a mixed culture (containing many species) by methods that separate the individual cells so that‚ when they multiply‚ each will form an individually distinct colony‚ which may then be used to establish new cultures with the assurance that only one type of organism
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the centre of the cells further away from the surface. Therefore‚ when dipped in sodium hydroxide‚ the larger cells will not be dyed pink all the way through like the smaller cells after the ten minutes. Materials: 1x1x1 cm agar cube 2x2x2 cm agar cube 3x3x3 cm agar cube 100 ml of sodium hydroxide Goggles Petri dish Scissors Timing device Tweezers Beaker Graduated cylinder Procedure: Refer to ’Nelson Science Perspectives 10’ pages 38-39‚ section 2.4; ’What Limits Cell Size?’.
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as to provide i) the nutrients for the survival of the plant cells‚ tissues and organs under culture and ii) the optimal physical condition of pH‚ osmotic pressure‚ etc. In the culture of plant cells formulating optimum type of medium favorable for in vitro culture was achieved many years ago. The Knop’s (1865) mineral solution was the widely used medium by early investigators. Gautherat (1939) developed callus culture medium from Uspenski and Uspenskaia (1925) nutrient solution. A systematic
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Biodegradation of Hydrocarbons from Crude Oil by Pseudomonas putida A Project done under the guidance of Dr. K. Bharathi Department of Biotechnology. Submitted to the faculty Of Department of Biotechnology National Institute Of Technology‚ Warangal (A.P) Submitted By Febin P. Nalpady‚ Anzal Rahman‚ Shruti Sharma‚ Sindhuja Nandiraju‚ Giraboina
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ANTIMICROBIAL ACTIVITY OF SOIL USED IN THE TREATMENT OF BACTERIAL DIARRHOEABYSITUMBEKO LIWELEYA(s213459531)Research Proposal Submitted in fulfilment of the requirements for the degree of BTECHBIOMEDICAL TECHNOLOGY | | FACULTY OF HEALTH SCIENCES | atNelson Mandela Metropolitan University | MODULE- RESEARCH METHODOLOGYDUE DATE- 16th MAY‚ 2013.SUPERVISOR- PROFESSOR N. SMITH | | Contents ANTIMICROBIAL PROPERTIES OF SOIL USED IN THE TREATMENT OF BACTERIAL DIARRHOEA
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an isolated bacterium can be identified. In this experiment‚ a single bacterial colony was isolated form Mycorrhizal spores‚ and further tests done on that colony. Sub culturing was done after each week to ensure that the bacterium has sufficient nutrients required for optimum growth that will last the duration of the entire experiment. A flow chart was created based on the results of the physiological tests in order to identify the isolated bacterium. After 4 weeks‚ the isolated bacterium was identified
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temperature as temperature is lower their growth is also slower and temperature above 30ºC may cause mutation in petite and sporulation is highly inhibited. 6. Isolating Single Colonies: Done by streaking cultures out on an agar plate to dilute the cells on the surface of the agar‚ overlapping streaks‚ separation or distribution of cells to form isolated single colonies. 7. Estimating the Number of Yeast Cells in a Culture: How to differentiate between molds and yeast culture: Traditional methods:
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tapai‚ cheese‚ bread and others. Starter cultures is used in these food production. A starter culture is a microbiological culture which actually perform fermentation. These starters usually consist of a cultivation medium‚ such as grains‚ seeds‚ or nutrient liquids that have been well colonized by the microorganisms used for the fermentation. These starters are formed using a specific cultivation medium and a specific mix of fungal and bacterial strains. microorganisms used in starters include various
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he root system is developed in the soil since soil can help establish their strand and protect the fragile roots from stressful conditions such as drought‚ nutrient deficiency‚ unfavourable temperature‚ etc. Hydrotropism is a directional growth response exhibited in terrestrial plants in which water or water vapour is the orienting stimulus. In hydrotropism the root caps perceive and respond to moisture gradient of the soil simultaneously which will then regulate the directional growth of the root
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Catalog Number 166-0003EDU Week 7: pGLO Transformation Introduction to Transformation In this lab you will perform a procedure known as genetic transformation. Remember that a gene is a piece of DNA which provides the instructions for making (codes for) a protein. This protein gives an organism a particular trait. Genetic transformation literally means change caused by genes‚ and involves the insertion of a gene into an organism in order to change the organism’s trait. Genetic transformation
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