Enumeration of Bacterial Contamination in Hamburger Meat from Unknown Sources C March 6‚ 2012 The importance of bacterial enumeration has become even more apparent in recent years due to the increasing numbers of harmful bacteria found in meat products. This process is the key to understanding the populations of microorganisms that contaminate the food supply. Much of the bacteria in meat has been shown to be resistant to multiple drugs; so disease-causing microbes
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turn the agar plate over and divide the plate into four quadrants and label the agar plate whether you used the E. coli or B. megaterium and number the quadrants 1 through 4. Please keep in mind that one pair will test the E. coli and Environment 1 or 2‚ and one pair will test B. megaterium and Environment 1 or 2. After‚ you will need to swab the E. coli and B. megaterium on two different nutrient agar plates using a sterile disposable inoculating loop. Remember not to dig in into the agar or the
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Microbes are everywhere. Objectives: The experiment performed in the Lab was isolation of microbes taken from us and the environment. We used Nutrient Agar which is a growth medium used to culture microorganisms or small plants and Sabourand Dextrose Agar plates used to cultivate moulds and yeasts. The objective of it was to demonstrate that microbes are everywhere. We expected to find a variety of bacteria‚ moulds and yeasts. We were introduced to aseptic techniques as they help ensure that
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for medicines and cure. Identification of bacteria is a multistep process because while some preliminary guesses can be made from the morphology of microbes on various differential agars‚ various other tests need to be done to differentiate and confirm their identity. RESULTS Bacteriology – When grown on MacConkey agar‚ A had abundant growth of pink punctiform colonies that are circular and moist. Microbes A are gram-negative cocci. B had moderate growth of yellow punctiform colonies that are circular
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Abstract Spore suspension of Bacillus pumilus was inoculated into universal bottles containing sterile distilled water in water baths at temperatures of 85°C‚ 90°C and 95°C. At specific time intervals‚ a sample was removed and spread on nutrient agar plates. The number of colonies formed was used to determine the D-value and z-value. The D-value for 85°C is 64.1 minutes‚ 25.7 minutes for 90°C and 8.2 minutes for 95°C while the z-value is 11.2°C. Introduction Bacterial endospores are
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of incubation‚ results were collected as growth or no growth for each temperature. Methods and Materials: Figure 1a: agar plate Materials 6 nutrient agar plates media grown overnight in Tryptic Soy Broth E. coli P. fluorescens B. stearothermophi lis Inoculum loop
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was place on a rack then the stain were apply. B. From agar cultures. 1. A drop of water was place in the center of the glass slide. 2. With sterilized inoculating loop to obtain a minute of bacterial culture from the agar cultures 3. It then mix with the drop of water and then proceeds as broth cultures. SIMPLE STAINING TECHNIQUES Material: 1. 24 hours broth cultures of a. E. coli b. Staph. aureus 2. 24 hours nutrient agar slants of a. Bacillus subtilis b. Pseudomonas aeruginosa
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Systematic Identification of Bacillus subtilis and Serratia marcescens Through a Battery of Tests and Plates Introduction: The purpose of this experiment was to use a systematic battery of tube tests and plates designed to lead to identification of two unknown bacterial species‚ from the combination of all results. A sample of bacteria was used‚ labeled “Sample 4”‚ from which both species was to be obtained‚ one gram positive and one gram negative. Table 1 is a list of the possible bacteria to
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confirm. Finally only MTCC cultures were used to prepare inoculum. Inoculums Preparation Primary isolation was initiated by nutrient agar plating and subsequently transferred to selective media like Pseudomonas Isolation Agar‚ MacConkey Agar and EMB agar for better isolation. Using sterile inoculation loop colonies of the test organism are transferred to 5ml of sterile nutrient broth and incubated at 37 0C overnight for 24hrs. Then this bacterial culture were suspended in saline solution (0.85%Nacl)
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coli)‚ Enterobacter aerogenes (E.aerogenes)‚ Klebsiella pneumoniae (K.pneumoniae)‚ Proteus mirabilis(P.miranilis)‚ Pseudomonas aeruginosa(P.aeruginosa)‚ and Salmonella typhimurium (S.typhimurium). The biochemical tests utilized were; Triple Sugar Iron Agar (TSIA) test‚ Sulfur Indole Motility (SIM) test‚ Methyl red test and Voges-Proskauer (MR-VP) test‚ Citrate test‚ Urea test‚
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