of LB agar. We then put 250 µn of CaCl2 transformation solution into two micro test tubes for the purpose of changing the bacterium’s cell wall to allow the pGLO plasmid to enter more easily. Second‚ we placed one colony of E. Coli from our original plate into each of the micro test tubes. Subsequently‚ we extracted a loopful of pGLO plasmid with a sterile loop and then placed it in one of the micro test tubes. We incubated the tubes on the ice for ten minutes while we prepared four new agar plates
Premium DNA Gene Bacteria
• Eye protection • 250 mL beaker • Timing device • Scoopula • Ruler • Scalpel • Sodium hydroxide solution • 3 different sized cubes of phenolphthalein agar • Paper towels Purpose 1. Put on eye protection 2. With the scalpel cut the block of phenolphthalein agar in to a 1x1x1 cm cube (Cube A)‚ a 2x2x2 cm cube (Cube B) and a 3x3x3 cm cube (Cube C). 3. Pour enough sodium hydroxide solution in to the beaker to cover the
Premium Chemistry Diffusion Osmosis
synthesis of ATP (Hertfordshire‚ 2011). Without ATP cellular functions fail and then die. Research Question: How does increasing the concentrations of Dettol Antiseptic Liquid from 0% to 100% affect the growth of Escherichia coli bacteria on the agar plates? Hypothesis: As the concentration of Dettol Antiseptic Liquid increases the inhibition of bacterial growth around the antiseptic disks also increases. Variables: |Type of variable
Premium Bacteria Immune system Inflammation
bacteria’s genome (Hanahan‚ Studies on transformation of Escherichia coli with plasmids‚ 1983). To see the reaction of this plasmid on the cells‚ bacteria treated with the plasmid were grown on two separate agar plates containing LB nutrient broth and ampicillin‚ and another containing LB nutrient broth‚ ampicillin
Premium DNA Gene Bacteria
ii) Tube of sterile distilled water. iii) Petri dishes with nutrient agar iv) China Marker v) Object to be tested (all are from the front of the class room): (1) Teachers Chair (2) Computer Keyboard (3) Computer Mouse (4) Teachers Desktop b) Method: vi) draw‚ with a china marker‚ on the outside of the agar portion‚ not the lid‚ of a Petri
Premium Bacteria Antiseptic
Lab Report T 1:35 Prof. Clemente Fernandez MCB2010L 11/19/13 Cristina Carvajal Fatima Hussain Nordia Johnson Jessica Rignack Amoeba: It is a unicellular protozoan that lacks a definite shape. Live in both fresh water and marine habitats. Some are found in soil‚ and many have adapted to parasitic life on the body of marine animals or internal organs of both aquatic and terrestrial animals. Amoeba reproduces asexually by binary and multiple fissions. They eat algae‚ bacteria‚ other
Premium Bacteria
suspension were put into a 1/3 stength of Hay Infusion Agar. Then‚ 0.4 mL of 24-hour old E. coli was added to serve as nutrient source of the Dictyostelids. The plates were incubated at 24°-25°C for 24 hours and were observed periodically the second day. Using a compound light microscope‚ the development of sorocarps were observed and noted. From the results collected‚ each stage on the life cycle of Dictyostelids was observed. When the nutrient source was abundant‚ generally‚ solitary amoebae were
Premium Slime mold Dictyostelid
if organisms possess this trait is useful in microbial identification schemes. The Ziehl-Neelsen method has endured as a reliable and effective way to demonstrate the acid-fast bacteria. Materials: 18-20 nutrient hour agar slant culture of Staphyloccus aureus 4 day old nutrient agar slant culture of Mycabaterium smegmatis Hot plate and beaker Inoculating needle and loop Bibulous paper Carbolfuchsin Acid- alcohol Methylene blue Staining procedure: Fill beaker 2/3 full with water. Place
Premium Bacteria Cell wall
procedure performed was an isolation of my unknown bacteria with the goal of obtaining a pure culture. This was done by streaking the unknown onto a nutrient agar plate using the streak method. The plates were incubated for two days and the bacterium was able to grow. I studied the bacteria based of its physical characteristics of how it grew on the agar. I began my quest by conducting a Gram stain on my bacteria. I prepared a smear by placing a drop of water onto the center of a slide and then removed
Premium Bacteria Enzyme Staphylococcus
greater microbial diversity was present in hairy cap moss than in lichen‚ with a greater percentage of growth for each colony. This could be the result of the mutual symbiotic relationship that bacteria share with hairy cap moss‚ a greater source of nutrients available for bacteria in hairy cap moss in soil than in lichen on bare rock‚ or due to the temperature differences between the two sites being sampled. Introduction Lichen‚ or Cladonia sp.‚ is a plant that is made up of algae‚ bacteria and fungi
Premium Bacteria Organism Symbiosis