Keep bottle closed when not in use.If alcohol in bottle ignites place lid on bottle.In case of fire on bench cover with fire blanket.Inform laboratory staff if spill occurs | Nutrient agar plates | Non-hazardous | Wear personal protective clothing‚ including gown‚ closed shoes & safety glasses.Dispose of contaminated agar in appropriate‚ labelled contaminated waste container. | Biological Hazard 7 | Risk (Harm) | Risk Control Measures | | | | Escherichia coli (Biotype 1) | Risk group
Premium Personal protective equipment Protection Occupational safety and health
ABSTRACT Nature has been a source of medicinal agents for thousands of years and an impressive number of modern drugs have been isolated from natural sources Plants used for traditional medicine contain a wide range of substances that can be used to treat chronic as well as infectious diseases. Clinical microbiologists have great interest in screening of plants for antimicrobial activities and phytochemicals as potential new therapeutics. The use of plant extract for medical treatments is enjoying
Premium Bacteria Antibiotic resistance Penicillin
urease. Casein hydrolysis tests for organisms capable of hydrolyzing casein via the casease. Materials and Methods Bile Esculin Hydrolysis The organisms Lactococcus lactis and Enterococcus faecalis were spot-inoculated on a bile esculin agar plate. The bile esculin agar plate is a both selective and differential medium contains primarily esculin. The plate was then inverted and incubated at 37 oC for 24 hours. Bile salts‚ the selective agent‚ can allow only Enterococcus and group d streptococcus to hydrolyse
Premium Starch Enzyme Bacteria
The results that were collected in lab #1 shows the relationship of surface areand volume in the artificial cells to the diffusion rate using the phenolphthalein-NaOH agar and the HCl solution. Lab #2 was a model of diffusion and osmosis‚ in which we filled the model cells with different solutions and determined the rate of diffusion. In lab #3‚ the results demonstrated the interactions between selectively permeable membranes‚ water‚ and solutes and how they are important in cellular and organismal
Premium Osmosis Chemistry Diffusion
aureus. Introduction: The purpose of this lab was to determine the identity of an unknown bacteria slant culture using a series of differential tests. The tests used to identify the unknown bacterial culture included: Gram stain‚ mannitol salt agar‚ coagulase tube test‚ and an antimicrobial susceptibility test. The tests selected were based on the results of a gram stain. Gram staining‚ the most commonly used differential stain‚ allows for the fast and easy detection between gram negative and
Premium Bacteria Staphylococcus aureus Gram staining
this study. All procedures‚ unless otherwise noted‚ have been followed and performed exactly as stated in the laboratory manual Leboffe & Pierce(1). Using the Quadrant Streak technique the unknown and ubiquity were streaked onto a trypticase soy agar (TSA) plate. This method was performed as stated in the Leboffe & Pierce lab manual(1). The TSA plates were incubated at 30 degrees Celsius for 48 hours. This process was repeated until isolated colonies appeared. At this point cell morphology was
Premium Bacteria Microbiology Microorganism
slants are used for two reasons. Firstly‚ the slant can be used to make sure that there is no contamination from the Nutrient Agar plate. Secondly‚ the second slant will become a stock culture to prevent the shortage of slants during performing the series of tests. Kliger’s Iron Agar tests can be used to determine multiple characteristics of unknown microorganism #17. Kliger’s Iron Agar slants also contains a pH indicator‚ phenol red‚ which can be used to test the presence of fermentation. If there
Premium Enzyme Metabolism Glucose
Bacterial Transformation I. Abstract In the lab‚ the purpose is to see if we could move genes using plasmid. As well as getting better understand of transformation methods using shock wave. To see the effects five trays are being used containing LB nutrient broth. The results showed that the LB‚ ampicillin‚ and arabinose with a positive pGLO had the most amount of growth compared to the other four trays. Although when there is arabinose there is no fluorescence‚ fluorescence was seen under UV lighting
Premium Bacteria DNA Molecular biology
5 minutes after inoculating. The turbidity of the broth is observed and compared with the control after 24 hours. Part B ( Effect of osmotic pressure on growth) 1) Label four plates of glucose agar containing varying concentrations of NaCl (0.5%‚ 5%‚ 15%‚ and 25%) and three plates of glucose agar containing varying concentrations of sucrose (0.5%‚ 15%‚ and 30%). 2) Escherichia coli‚ Pseudomonas fluorescens‚ Micrococcus luteus‚ and Saccharomyces cerevisiae were inoculated into each plate
Premium Bacteria
heard of. The materials we used to make this test possible consists of a sterile nutrient agar Petri dish‚ the bacteria Serratia marcescens‚ Erythromycin antibiotic disk‚ Ampicillin antibiotic disk‚ Penicillin antibiotic disk‚ sterile disk of blank filter paper‚ marking pen‚ long handled cotton swab‚ forceps‚ metric ruler‚ and a 37°C incubator. For our procedure we first filled our sterile Petri dish with agar‚ just so that the bottom of it was covered‚ then waited a day for it to condense.
Free Bacteria Antibiotic resistance Antibiotic