We successfully adapted a commercially available protein detection assay for the quantiWcation of PEI. All experiments described below were performed with linear 25-kDa PEI (Polysciences‚ Warrington‚ PA‚ USA) that we routinely use for the transfection of mammalian cells [4]‚ but it was possible to quantitate other PEIs (diVerent molecular weights; branched) with this method (data not shown). The mixing of a PEI solution with the Advanced Protein Assay (APA) reagent (Cytoskeleton‚ Denver‚ CO‚ USA)
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Bio 6 (Mon. Lab) 5/6/13 The Effects of Pepsin VS. Trypsin in The Digestion of Protein Introduction As food is mechanically and chemically digested through our oral cavity then passes through our pharynx and down our esophagus‚ our food then enters the stomach. The stomach‚ being the main organ for storage‚ also helps with breaking down our food‚ but in order to accomplish that our pancreas helps by excreting an inactive digestive enzyme called pepsinogen. Pepsinogen is the inactive form
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in Two Carbohydrate Solution | Objective: 1. To show the action of saliva in two carbohydrate solutions. 2. To show the action of hydrochloric acid in two carbohydrate solutions. Apparatus & Equipment’s: Boiling tubes Metal test tube racks Beaker Graduated plastic dropper Water bath‚~37°C Water bath‚~95°C Stop watch Test tube holder Materials: Carbohydrate solution
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Name: Piyush Sonawane Student Id: 901-767-730 The Big-O-Notation in Data structures Introduction:- The Big-O-Notation is done to test the efficiency of one algorithm or many algorithms of same code and the time duration it takes to run and to execute in the given input size or it can be also be defined as how algorithm or similar algorithms are execute more quickly in its worst case scenario or outcome. Example: A programmer would want to execute his program
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October 2‚ 2012 Biochemistry I Circular Dichroism and Secondary Structure of Proteins Proteins are vital to an organism life; they are involved in nearly all cellular functions. It is an essential part of enzymes‚ the cellular membrane‚ active transport‚ protein synthesis and wound healing. Because one relies so heavily upon proteins and its function the structure of proteins is also very important. “The way a protein will fold over its self-determines how it interacts with other chemicals in its
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How Primary Structure Determines Protein Function Proteins are everywhere. As enzymes‚ they catalyse chemical reactions. There are many different types of proteins: carrier and channel proteins which transport substances‚ receptor proteins which are recognition site for antibodies and hormones‚ structural proteins which support cells and tissues‚ and hormones which transmit information. Proteins are big molecules with large molecular masses that are made up of many amino acids; they’re‚ essentially
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Task-2 Part-2 Fibrous proteins /collagen: From my diagram I can observe the quaternary structure is 3 helical polypeptides which are looped around and is held by hydrogen bonds. The 3 stranded molecules runs parallel to others and the disulphide cross-linkages between the r-groups of the amino acid holds the molecules together forming fibres. Collagen molecules forms covalent bonds which are called cross links‚ with other collagen molecules next to it. The cross-links that form are shocked along
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Atmosphere: Thought the story‚ O’Conner presents us with several reoccurring themes of irony‚ realism‚ and revelation. She creates the atmosphere by beginning how the family interacted with each other and how they got along or how they didn’t. Baily and his wife tend to “ignore” the grandmother‚ because they feel like she always has something on her mind thus they had no reason to try and reason with her‚ while the children like to argue with grandmother‚ resulting further igniting her flame. O’Connor
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To examine three different ways of denaturing proteins. HypothesisIt was predicted that the physical characteristics of the egg white solution at room temperature would appear clear and normal like a raw egg white. This is because nothing would be done to the egg white. It was also predicted that when the egg white solution gets heated‚ the protein would denature if the temperature exceeds 65 °C. The protein would solidify‚ turn opaque‚ and turn white in colour. This would happen because the heat
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essentially the building blocks of protein. There are 20 different kind of amino acids that form protein but only three of them are essential for humans. those three are phenylalanine‚ tryptophan‚ and valine. These amino acids make up about 75% of the body and the essential amino acids need to be ingested every day or else protein degradation can occur. 2) List all of the roles proteins have in our bodies. page #202 There are many different type of proteins that are configured for different kinds
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