Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Table of Contents I. Introduction * About the author page 2 * Preface page 3 II. Laboratory Techniques * Cell Culture Technique page 4-5 * Sterile Technique
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What is it? Sickle cell anemia is a type of blood disorder that can be inherited from parents. This is when red blood cells that are meant to carry oxygen around the body become deformed. Normal red blood cells are round and disc shaped‚ however‚ when they become sickle red blood cells‚ the deform and change shape into a crescent-like shape or sickle shape. The sickle cells have a shorter life-span than a healthy red blood cell and are not replaced as quickly as normal; this leads to a shortage
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CE121: Construction Materials Laboratory Report No. 1 Particle Size Distribution Van Joseph E. Casalme 2010-14928‚ BS Civil Engineering Institute of Civil Engineering‚ College of Engineering University of the Philippines Diliman‚ Diliman Quezon City 1101 Submitted to: Engr. Christian R. Orozco ABSTRACT This test (ASTM C136-06) determines the grading of materials being used as aggregates using two parameters (coefficient of uniformity and gradation) from particle-size distribution
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Life Science Lab. A. Arnold Tuesdays @ 2:30 September 30th‚ 2011 Lab Report: Nuclear and Cell Division. PART A: Stages of Mitosis in my own words. 1. Interphase: DNA has formed already‚ but it remains in the simple form of chromatin. Chromatins are structures that are loosely coiled in the cell.3 I also observed during my lab that this was the only stage where I could still see a nucleus and nucleolus intact within the cell; this is because it’s the only stage where the nuclear membrane has
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Acid Base Titration Purpose: The purpose is to calculate the molarity of a NaOH solution by titrating the base with 5mL of standard HCl solution in each trial. By adding the base with unknown molarity to the acid with 0.10M the molarity of NaOH can be calculated. The base‚ NaOH‚ helps bring the pH of the acid‚ HCl‚ closer to seven‚ which neutralizes it. When using the buret the amount of NaOH used is able to be determined. Then by writing a balanced chemical equation and using the titration
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Respiration: How Cells Harvest Chemical Energy 1. A cell must work just to maintain its complex structure‚ for order is intrinsically unstable. Because of entropy (the second law of thermodynamics)‚ which states that any enclosed system tends toward disorganization‚ cells must constantly use energy to keep themselves in order. If the cell was to stop using energy and let itself go then it would eventually get so disorganized that it wouldn’t function properly. 2. With the help of enzymes‚ the cell systematically
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Bubble Inside a Bubble Materials • • • • • • • • Granulated sugar (we had our best results using Imperial Sugar and Dixie Crystals) Dish soap Water Tablespoon Scissors Pipette Cup Adult supervision Bubbles form because of a combination of water’s hydrogen bonds and the oily film you can see shimmer in the light. The oily film you see is actually two separate layers of soap attached to‚ and surrounding‚ hydrogen-bonded water. Solar Oven S’mores Materials • Pizza box • Two clear sheet protectors
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Abstract This lab’s purpose was to test the patterns of percolation in various places. This lab tested how soil would absorb water based on different places. Distance from a group of trees‚ distance from a swamp‚ and different types of soil were tested. From the results collected‚ you can not prove‚ but you can infer that the ability to absorb increases as you go farther away from a swamp‚ and a group of trees. The ability to absorb also increases based on how much silt is in soil. Introduction
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INTRODUCTION There are many reasons for knowing the identity of microorganisms. The reasons range from the knowing the causative agent of a disease to knowing the correct microorganism in order to make antibiotics. This study was done by applying the following methods; OF Glucose‚ Indole Production‚ and Malonate Utilization test for the identification of an unknown bacterium. The methods will assist in determining the unknown bacterium found in a 55 year old male that was passing blood and mucous
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of basic units of matter called atoms. When atoms are bonded together they make up a molecule. Often associated with nonliving things are inorganic molecules‚ and associated with organisms are biomolecules. Today in lab‚ a series of tests were ran. Tested were the biomolecules of cells which are lipids‚ sugars‚ proteins and starch. The purpose of these tests were to test for the presences of cellular biomolecules. Some of the things that fat consist of are one glycerol and three fatty acids. Proteins
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