Lab #6: pH Effects and Enzyme Concentration on Catecholase Activity Abstract: This experiment examined the effect on how the changes in pH balance of potato juice affected the enzyme activity‚ structural stability and solubility. Introduction: Enzymes are macromolecules that allow chemical reactions to occur. They function as biological catalysts. A catalyst is a substance that is involved in‚ but not changed by or consumed in‚ a chemical reaction. The amount of energy required for a reaction
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because the high amount of salt causes more dehydration. Variables: Independent- Salt Solution Dependent- Mass of Potato Control- Tap Water Materials: •Tap Water (50 ml for each cup) •Salt •Beaker •3 cups •3 pieces of potato •Scale Procedure: Step 1: Gather Materials. Step 2: Weigh each potato separately on the scale and record weight in grams on table. Step 3: Put each potato in a separate cup and add 50 ml of water to each cup‚ measure with beaker. Step 4: To create the 40% salt solution
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Germans‚ימ’’ש ? What is this that’s worth his whole life? A potato. Not even a whole one‚ but half of a potato‚ smuggled out of the kitchen. He had risked his life‚ both by going there without permission‚ and by taking the potato. But it’s so very important to him‚ so he did it anyway. And now‚ here he is‚ waiting impatiently to go into his barracks. Finally‚ the endless roll call is over‚ and he sighs with
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References 13 Journal 14 1 Design 1.1 Defining the problem Research Question This lab will be driven by the research question; do changes in temperature (from 0˚C‚ 7˚C‚ 21˚C‚ 37˚C‚ and 90˚C) have an effect on the activity of the enzyme catalase (found in beef liver) in the breakdown of hydrogen peroxide? Hypothesis If liver is placed in different temperatures of 0˚C‚ 7˚C‚ 21˚C‚ 37˚C‚ and 90˚C and hydrogen peroxide is added to each piece of liver‚ then the liver placed in 37˚C will
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affected by environmental conditions. Researchers tested the level of potato extract enzyme activity with 1-11 pH‚ varying temperature‚ catechol solution‚ hydroquinone solution‚ and different measurements of catechol. In Figure 1A and 1B‚ pH levels were tested with potato extract to see how pH would affect the amount of Benzoquinone is formed in the potato. Although it was hypothesized that enzymes would form Benzoquinone better in acidic pH levels‚ the absorbency recorded stated that the higher the absorbency
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concentrated‚ which was hard to identify the shape of the bacteria‚ while other sections were less concentrated and was easier to analyze the shape. The next step according to the result‚ will be a catalase test.
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Enzyme Activity and Oxygen Production Throughout this report you will gain information as to how temperature effects the amount of oxygen produced in an enzyme- catalase experiment. In the experiment we used liver extract as a catalase and created a chemical reaction within a reaction chamber between the catalase and hydrogen peroxide as well as three different controlled temperatures. In the procedure below there will be a step by step process as to how the experiment was created. As
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Summary The poem begins with our speaker at his desk‚ his pen poised to begin writing. He gets distracted by the sound of his father outside‚ working in the garden‚ and this sends our speaker into a spiral of memories about his father working in the potato fields when the speaker was a young boy. The memory stretches even further back to his grandfather and the hard work he did as a peat harvester (there’s all kinds of hard work going on). Eventually‚ our speaker snaps out of his daydream‚ and we find
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Armadillium vulgare taxis response to different pH levels. Abstract The terrestrial isopod‚ Armadillium vulgare is commonly referred to as a slater or pill bug. Since transitioning from the sea to land and originally colonizing in Mediterranean regions‚ it has adapted throughout evolution to inhabit local microhabitats. The pill bug is bound by several parameters and also has specific requirements that need to be met for optimal biological functioning. As such‚ behavioural and physiological
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pH & Enzyme Action Aim: To inspect the effects of the pH on enzymes. Apparatus: 100 cm³ Beaker 3 – 5cm³ Syringes 2 Test Tube Racks with 8 Test Tubes Stop-watch Ruler Dropping bottle of detergent Marker Pen Masking Tape 400cm³ Hydrogen Peroxide 200cm³ Liver Catalase Solution 100cm³ of following Buffer Solution – pH5 pH7 pH9 pH11 Method: The materials were collected. The test tube rack one with 4 test tubes had been labelled A to D. The 2cm³ of each buffer solution
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