------------------------------------------------- COLORIMETRIC DETERMINATION OF pH ------------------------------------------------- Abstract This experiment examined the result of the various color indicators combined with different buffer solution‚ thereby testing the precision and accuracy of determining the pH value colorimetrically. Colorimetric method is a simple and practical procedure with an important advantage: they do not get out of order as from time to time do the pH-meters. Four buffers (Acetate buffer‚ phosphate
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Determination of pH Almira‚ Faerie Carleen Lucile L. Gallardo‚ Charlotte O. Group #6‚ Chemistry 18.1‚ MHEG1‚ Ma’am Arlou Angeles September 23‚ 2013 I. Abstract The acidity of the four unknown solutions were determined with the use of colorimetry using McIlvaine’s buffer solutions varying in proportion of its constituents (disodium phosphate and citric acid). These buffer solutions were subjected to the addition of corresponding pH indicators and the variation of colors depending on its pH level was
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Experiment 3: Soil pH measurement Introduction Aims The aims of the experiment were to determine the pH of a variety of soils which included sedentary‚ 3:2‚ sand‚ organic matter with the means of a pH meter at various soil water ratios and with or without calcium chloride. Method and Materials Samples of sedentary soil‚ 3:2 soil‚ sand‚ organic matter and compost‚ 100ml vials(x12)‚ bottle of distilled water‚ analytical balance‚ pH meter‚ 2 buffer solution of known pH‚ 0.25M calcium chloride
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Introduction When using different methods to measure pH levels there are some tools that can be useful. Some more than others but by putting into action the different methods it may determine which tools will work best and give the best results when testing the pH within a solution. The pH‚ which stands for the proportion of hydrogen ions in a solution‚ could be acidic (acidosis)‚ neutral or basic (alkaline). The pH scale goes from numbers 1 through 14. A pH of 7 is neutral; meaning the amount of hydrogen
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The Effects of pH on Slime Mold Dg Nicole Harris BIOL181 – 059 1 December 2011 The theory that slime mold is attracted to acidic solutions was examined by the slime mold‚ Dg. Dg’s preference of pH was examined by using a variety of pH solutions in a petri dish. The data suggests that the growth of fruiting bodies observed in pH 9 was comparable to that of pH 4. INTRODUCTION The 2002 scientific journal‚ “The Costs and Benefits
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effect of three different pH levels (4‚ 7‚ 10) on the rate of germination of spanish beans Research Question: What is the effect of three different pH levels (4‚ 7‚ 10) on the rate of germination of spanish beans? Independent Variable: The different pH levels in the water would be the independent variable in this lab. Three different pH levels were tested‚ 4‚ 7 and 10‚ both 4 and 10 were chosen because they are 3 pH levels away from 7 which would be the regular pH level of water. We measured
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Title: pH and buffer solutions Aim This experiment was carried out to determine the role of buffer solution and the factor which affect the buffer capacity. Besides‚ this experiment was carried out to investigate the solubility of protein casein over a range of pH concentration. This experiment also was carried out to determine the isoelectric point of the casein and the effect of the isoelectric point toward the casein solution. Methods Verification of the Henderson-Hasselbalch equation
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Red Cabbage Indicator Aim – To create a pH indicator out of a red cabbage solution and to construct a basic pH scale to determine the pH of unknown solutions. Materials - • red cabbage leaves • 250 mL beaker • hotplate or Bunsen burner‚ tripod‚ gauze mat and bench mat • 10 test-tubes – equal size • test-tube rack Methods - Part A: Making the indicator 1. Tear up one or two red cabbage leaves‚ and place them in the beaker with enough water so that the cabbage is just covered. 2. Heat the beaker
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Self-design Practical: The effect of pH on enzyme activity Rationale Amylase is an enzyme involved in the digestion system which catalyses the breakdown of starch into sugars. It is not only present in human saliva but also in the pancreas‚ where it hydrolyses dietary starch into disaccharides and trisaccharides which are converted by other enzymes to glucose to supply the body with energy. pH has an effect on the activity of all enzymes‚ including amylase. As the pH level increases‚ the enzyme activity
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the effects of an incorrect pH level. pH measures the concentration of hydronium ions and can be modeled by the function p(t) = −log10t. The variable t represents the amount of hydronium ions; p(t) gives the resulting pH level. Water at 25 degrees Celsius has a pH of 7. Anything that has a pH less than 7 is called acidic‚ a pH above 7 is basic‚ or alkaline. Seawater has a pH just more than 8‚ whereas lemonade has a pH of approximately 3. 1) Create a graph of the pH function either by hand or using
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