Alpha Amylase with Starch at Specific pH ’s and Temperatures Page 1 Abstract Enzyme ’s are used as catalysts in certain reactions. They help lower the activation energy needed for the reaction to go to completion. At optimum temperature and pH the amount of collisions of substrate and enzyme is at its highest‚ any deviation from the optimum temperature and pH will result in the denaturization of the enzyme. The purpose of this experiment is to find the optimum temperature and pH for the
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Starch Diffusion in a Caterpillar’s Digestive Tract The object of this particular experiment is to gain knowledge on diffusion across a plasma membrane‚ which is selectively permeable. Diffusion is the movement of a substance from a region of high concentration to lower concentration. Experiment 2 was performed to see how the substance’s‚ starch‚ and diffusion process across a plasma membrane is affected by amylase. We prepared a simulated digestive tract of a caterpillar‚ as the plasma membrane
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Temperature and pH will both effect and change the active site of an enzyme. Denaturing‚ the changing of the shape of an enzyme‚ will occur if a big enough change in temperature and pH to go away from that enzyme’s optimal conditions. Each enzyme has optimal conditions which is the best temperature and pH for that enzyme to function. When temperature and pH move away from those conditions the enzyme could slow down its rate of activity
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effect of pH on amylase activity This practical allows you to: * discover how pH affects the rate of an enzyme controlled reaction * evaluate the experimental procedure Procedure SAFETY: Follow your teacher’s instructions for handling the solutions. Wear eye protection when handling the iodine solution. Investigation * Place single drops of iodine solution in rows on the tile. * Label a test tube with the pH to be tested. * Use the syringe to place 2 cm3 of amylase into the
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Activity of Salivary Amylase Domingo‚ Guray‚ Hugo‚ Lorenzo‚ Mohammad Isa Intro Because everything has a start Catalysis The process of increasing the rate of reaction with the use of a catalyst. Catalyst – any substance that increases rate of reaction upon addition to a certain reaction Page 3 Enzymes Act on substrates in a reaction Highly specific Breaks down complex macromolecules‚ synthesizes compounds essential for the cell Active site Enzyme-substrate
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enzyme‚ ’-amylase in starch-iodine solution. We will be testing the relationship between enzymatic reaction affected by temperature and pH. Through the testing the enzyme at different temperatures‚ and different pH levels; it would determine at which temperature and pH level the enzyme worked the most efficiently. Analyzing absorbance of the solutions with spectrophotometery will determine the reaction rate. To test the optimal pH‚ the starch and a buffer were combined at a specific pH level and
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found in and out of cells and lower the activation energy of a reaction. Amylase is the enzyme which catalyses starch hydrolysis. Alpha amylase and beta amylase are two types of amylase enzymes. The amylase which is the most commonly found in the human body is the alpha amylase. Beta amylase is mainly found in bacteria‚ fungi and plants. Amylase breakdown starch into maltose. During the process of hydrolysis‚ Amylase degrades starch by splitting the long glucose units into smaller intermediates and finally
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Abstract This experiment was carried out to monitor the ability of the Barley Amylase Enzyme to effectively break down starch in solutions that are increasing in neutral pH. To do this the experiment was carried out so that tubes containing a reaction solution of the Amylase enzyme and starch were simultaneously mixed. The reactions were then introduced to I2-KI‚ which stopped the reactions‚ at two minute intervals. Each of these trials was repeated three times to ensure proper accuracy. After
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Tomas‚ Manila Philippines Abstract Salivary amylase‚ found in humans‚ is enzyme that catalyzes the hydrolysis of starch into simpler compounds. Its enzymatic activity is affected by several factors‚ such as temperature and pH. The rates of enzymatic activity of salivary amylase in different temperatures and pH were measured and resulted to be very near 50 C and 7 respectively. However‚ due to some errors that were committed‚ the expected optimum temperature was not achieved. Introduction Of
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Biology- Change in pH Aim: To investigate the affect that change in pH levels has on a particular enzyme‚ in this case amylase. Hypothesis: In this investigation I expect as the pH reaches the optimum level‚ the rate of reaction will be fastest‚ compared to other pH levels. It is also suspected that after the enzyme has reached optimum level the enzyme activity will decrease. Through further study of the optimum level of amylase I found that the enzyme usually has an optima pH of 8. It is known
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