Alyssa Caparelli Organic Chemistry 12A Professor Alston October 28‚ 2014 Isolation of Chlorophyll and Carotenoid Pigments from Spinach Purpose The purpose of this experiment was to isolate ß-carotene‚ chlorophyll-A‚ and chlorophyll-B from spinach using column chromatography. Spinach was dehydrated using ethanol‚ and the pigments were extracted with dichloromethane. The spinach extracts were dried using CaCl2. Then‚ the solid pigments were run through a column using a non-polar solvent‚ hexane
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cheese. Lactic acid bacteria(LAB)‚ a bacteria that can be found in the production of cheese‚ its stress gene was investigated in the experiment by using various biochemical and genetic techniques to identify and extract. The characterisation of the strain illustrates how identification of strains differ using different methods‚ such as gram stain and 16s rRNA screening. After the characterisation‚ the stress gene isolation assist the further understanding of the gene on LAB be giving different stress
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Objective: The purpose of the lab is to learn filtering techniques. Specifically‚ to form and filter a calcium carbonate precipitate using a Buchner funnel. Procedure: a.) Prepared a mixture of .5 M calcium nitrate (45 mL) and .01 M sodium carbonate by combining and stirring the two liquids in a beaker. Allow the mixture to stand. b.) Prepare the filtration assembly. The assembly consists of a tapered flask with a buchner funnel inserted in the top. The funnel has a rubber stopper attached
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Chemistry lab report Experiment 32 Voltaic Cell Measurements Dinmukhamed Yeraly Partner Azamat Bashabayev General Chemistry II lab Instructor Rostislav Bukasov Nazarbayev University Introduction Most of the chemical reactions can be classified as redox reactions‚ which include two half reactions‚ oxidation and reduction respectively. To measure the tendency for a readox reaction to occur‚ special apparatus called voltaic cell can be used. It is made by connecting a voltmeter between
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Research Question How will the addition of different pH buffers to amylase affect the rate of starch digestion measured using starch and iodine? Introduction Amylase is an enzyme found in human saliva and pancreas. It is the digestive enzyme that is needed to breakdown starch molecules. Amylase must be kept at certain conditions to function at its optimum level. This experiment will explore the effect of pH (1‚ 4‚ 7‚ 10‚ and 14) on the function of amylase by using starch and iodine. Usually
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INTRODUCTION: Carboxylic acids are organic acids characterized by the presence of a carboxyl group‚ -COOH. This acid acts as a weak acid‚ which can react with a strong base. Carboxylic acids form hydrogen bonds with many water molecules and are more soluble with one to four carbon atoms. Also‚ it may have an R group that consist of hydrogen or an alkyl group that changes its water solubility. Carboxylic acids with low molecular weight have odor at room temperature and higher molecular weight
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The purpose of this lab was to determine the effects of changes in variables such as temperature‚ pH and concentration on catalase enzyme activity. Proteins are organic compounds made up of amino acid subunits consisting of carboxyl and amino functional groups‚ linked together by peptide bonds through condensation reactions. They are an essential part of all living organisms. Enzymes are “biological catalyst used to speed up chemical reactions (Athanasopoulos‚ 2014). Normally‚ for chemical reactions;
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OLD COINS TURN TO GOLD DESIGN: Problem or Research Question: How does zinc effects the color change in a copper penny? Hypothesis: If copper and zinc comes together‚ then it will form brass‚ which gives gold color to copper penny. Variables: There were no variables at this experiment PROCEDURES: Materials: Zinc (SN) filling‚ 3M NaOH solution‚ Copper penny‚ tongs‚ Hot plate‚ 100 ml beaker‚ 250 ml beaker‚ Bunsen burner‚ Water‚ Spoon. Procedure: First‚ we turned on the hot plate.
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Lauren Sullivan Plants Imperfections Abstract The purpose of this experiment was to plant different crosses and observe the different phenotypic ratios the plants present. The procedure of this experiment was plant six different crosses and water them correctly so that we could observe the different phenotypes and compare them to Mendel’s proposed ratios. Mendel‚ who had studied peas‚ did a similar experiment and came up with specific ratios that a monohybrid and dihybrid cross should show. His
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Abstract……………………………………………………………………………………………2 Introduction………………………………………………………………………………………..2 Background………………………………………………………………………………..2 Objectives…………………………………………………………………………………2 Scope………………………………………………………………………………………3 Theory review……………………………………………………………………………………..3 Design of report…………………………………………………………………………………...5 Procedures…………………………………………………………………………………………5 Results……………………………………………………………………………………………..6 Discussion…………………………………………………………………………………………6 Conclusion………………………………………………………………………………………...7 Reference………………………………………………………………………………………
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