Objective: The objective of the lab is to separate the caffeine samples we are using‚ using thin layer chromatography. The solvent we are using for the separation is 3:1 mixture of Chloroform and Acetone. Principle: Thin layer chromatography (TLC) is an important technique that is useful for separating organic compounds. TLC is often used to monitor the progress of organic reactions and to check the purity of products. Separations in Thin layer chromatography involve distributing a mixture of
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Wave Nature of Light Objective: The purpose of this lab is to investigate interference‚ otherwise known as the diffraction of light. A beam of light acts a wave‚ and we are able to use equations so calculate the wavelength of the light used. The diffraction of a straight edge demonstrates that light waves bend around straight edges‚ allowing light to enter an area of shadow. When waves are superposed‚ they reinforce each other when crests are in phase and cancel out when they are not in phase
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LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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PEES 4200W- Physiology of Exercise Lab #2: Metabolism and Energy Expenditure 1. The Respiratory exchange ratio is the ratio of the volume of carbon dioxide produced and oxygen consumed. The ratio indicates the energy that the subject is expending for indirect calorimetry‚ how efficient the subject ’s body is at utilizing the oxygen inhaled‚ as well as the main substrate being used for energy during varying intensities of exercise. During rest the volume of carbon dioxide was 0.73L/min‚ the volume
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(gained or lost) an electron. (Pick one) 17. An anion is an atom that has (gained or lost) an electron (Pick one) 18. When a cation bonds with an anion‚ that bond is referred to as a/an _ionic_ bond. 19. Circle the molecules that are classified as compounds (H‚ H2‚ H2O‚ C6H12O6‚ N2) 20. Find the name for each element using the periodical
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The Synthesis of Organic Aspirin ABSTRACT: The purpose of this experiment was to find out how a reaction undergoes for a globally known painkiller called aspirin‚ and to become familiar with achieving successful yields. Aspirin is synthesized from salicylic acid and acetic anhydride. Those two chemicals are mixed together along with sulfuric acid to form a crude solid. Filtration is used separate the impurities from the crude aspirin. To get purified aspirin‚ the precipitate was heated until all
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chemicals before use. Abide by specific warnings and directions. 3. Collect all materials needed for a procedure before proceeding. 4. Perform reactions under the hood when directed. Chemicals may be weighed and prepared at balance or lab tables‚ but tests should be carried out under the hood. 5.Acids and caustic chemicals are stored in the hood. Please do not take these chemicals from the hood. Procedure: PART 1: Metathetical reactions Precipitation reactions A1. Add a
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concentrations of solutes in and out of the cell. Filtration is what is used to remove solid particles and they can be removed by passing through a liquid and gas. The information above is very important because it is exactly what everything in this lab will be about. It explains in detail what every single little definition and important
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of Chemical Engineering CHME 426 –Chemical Engineering Laboratory III Title Page (Full Report) Title of the Experiment: Reaction through three CSTR in series Submitted by: Group (4) Section: Female 1. Name: Amina Ali ID: 200550284 2. Name: Duaa Tabarak ID: 200553858 3. Name: Mariam Rustom ID: 200552242 Date of experiment: 31st March‚ 2010 Date of submission: 11th April‚ 2010 Grades: Report presentation………………………………………… /15 Abstract and Objective(s)……………………………………/10 Introduction
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Data collection Quantitative Data Raw Data Table 1: Table showing the mass of the amount of unknown acid X measured in grams (±0.001g) Table 2: Table of reading of the burette initially filled with 25mL of 0.201moldm-3 sodium hydroxide (NaOH) to titrate 25mL (±0.03mL) of unknown acid X in mL (±0.05mL) after each titre. Reading on the burette initially filled with 25mL of 0.201moldm-3 NaOH (±0.05mL) First titre 21.3 Second titre 18.2 Third titre 15.2 Fourth titre 12.0 Qualitative
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