In this lab we employed various assays utilizing a biuret reagent‚ coomassie brilliant blue reagent‚ and ultraviolet light in order to determine the identity of six unknown solutions and the concentration of a bovine serum albumin sample. We were given three samples that lacked protein‚ and three samples containing proteins‚ and using a spectrophotometer we assessed the amount of light absorbed versus the light transmitted‚ based on the principles of the Beer-Lambert Law. The three proteins used
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The lab was to include a purpose‚ procedure‚ data/observations‚ all reactions and side reactions written out with qualitative data beneath each product and reactant except H20‚ and a summary. The purpose of this experiment is to observe the qualitative aspects of a series of reactions involving copper. Procedure 1.Measure about 1g of solid copper. 2.Place Cu in Erlenmeyer flask and place flask under fume hood. 3.Add dropwise 15M HNO3 until solid copper is completely reacted. 4.Place flask
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Every cell‚ whether it belongs to a plant‚ bacteria or a human‚ requires energy from varying food sources to survive. However‚ when cells break down food into its sugar components‚ i.e glucose‚ they cannot use the components directly because glucose holds a lot of energy it would be inefficient to use for reactions that don’t need a lot of energy. Imagine you want to buy pencils for $5 from a store that won’t give change‚ it is more economical to use a $5 or $10 bill than a $100‚ that way there is
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If I want to target the peptide to different compartments‚ I would use specific signal sequences or uptake-targeting sequences encoded into my peptide. Signal sequences contain information that will target the peptide to a particular organelle. The information is usually encoded within amino acid sequences of the protein itself‚ usually within the 20-50 amino acids. Also‚ each organelle has specific set of receptors that bind only to the specific signal sequences‚ thus making sure of target specificity
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Aspirin or acetylsalicylate acid is a compound that is widely used in medicine. The use of aspirin in medicine began long before the active reagent was removed from its natural source. The first documented use of aspirin was found in the writings of Hippocrates‚ the so called father of medicine. Hippocrates prescribed that willow bark could be used to remedy a wide range of conditions such as pain‚ fever and inflammation. Scientists began to seek to extract the active compound from willow tree bark
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Angelina Mass-Valderrama CHM1032 summer ‘12 Graphing and Estimating Formal Lab Report Introduction: Today scientists put acquired data into a form of a graph. This said graph is designed to help make predictions and furthermore‚ study and understand the experiment and its contents at hand. The Graphing and Estimating lab involves just that. The lab is designed to collect data from several tests involving burn time of a candle. Oxygen‚ O2‚ is a kind of gas. A lot of the air we breathe
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ANALYSIS OF ASPIRIN TABLETS The aim of this investigation is to determine the percentage by mass of aspirin aspirin present in different commercial preparations and to find out‚ which the best value is‚ using a neutralization reaction followed by a direct titration with NaOH. Chemicals Used: 100 ml of distilled water in a wash bottle 50 ml of 0.100 M NaOH 40 mL of 96% ethanol phenolphthalein as an indicator (3 drops per titration) 325 mg non buffered aspirin tablets Materials Used: One
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1. Aim To determine the percentage of aspirin in different commercial preparations and to find which is the best value for money 2. Hypothesis The greater the percentage of acetylsalicylic acid (ASA) in the tablet‚ more powerful and efficient the aspirin would be due to the way aspirin works in human’s body. Aspirin is often used to alleviate the pain‚ and the sensation of pain is mainly attributed to a chemical substance called prostaglandins‚ which is responsible to send a strong signal
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The Grignard Synthesis of Triphenylmethanol Organic Chemistry Lab II March 19‚ 2012 Abstract The purpose of this experiment was to synthesize the Grignard reagent‚ phenyl magnesium bromide‚ and then use the manufactured Grignard reagent to synthesize the alcohol‚ triphenylmethanol‚ by reacting with benzophenone and protonation by H3O+. The triphenylmethanol was purified by recrystallization. The melting point‚ Infrared Spectroscopy‚ 13C NMR‚ and 1H NMR were used to characterize and confirm
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is associated with lactase persistence is the located upstream of the LCT gene. In this mutation a single nucleotide polymorphism changes a cytosine into a thymine that then can be detected using the polymerase chain reaction technique (Biology 225 lab manual‚ S2017). In this experiment amplification Refractory Mutation System PCR was used to detect this nucleotide change. Two primers were used in two different PCR reactions‚ one to detect the wild type allele and the other to detect the mutant allele
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