Abstract Some bacteria are able to go through transformation making new combinations of genes. Transformation is a way of gene variability in bacteria. This experiment is based on the transformation mechanism of bacteria and gene regulation. The bacteria used for the experiment was Escherichia coli and the genes introduces for the transformation were: gfp and bla by a pGLO™ plasmid. After the insertion of the target genes and growing the bacteria on specialized LB media‚ it could be seen that the
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I wonder why I Wonder I was born the 9th of August‚ 1994. I’ve always wondered if my premature birth had anything to do with my mother’s stress before I was born. Could it have been the earthquake? Could have it been the stress of having to wait a child for 13 years? Could it have been caused by the argument that my mother had with my aunt? The 1994 Bolivia earthquake occurred on June‚ 9. The epicenter (center) of the earthquake happened 200 miles from La Paz‚ Bolivia. It was one of the largest
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The Birth of Freedom Just recently we celebrated the Sesquicentennial of the Civil War‚ a milestone in civil rights. We decided to base our National History Day Exhibit on this milestone‚ more specifically the Emancipation Proclamation. The Emancipation Proclamation is important to us because we ourselves would be considered a minority in a new nation. During the creation of the new nation our ancestors probably faced a difficult society unlike their own. The battle for civil rights lasted more
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Seven Transformations of Leadership Throughout this whole semester‚ we’ve learned about the tools‚ mannerisms‚ and styles of an effective leader‚ but I never thought that there were seven unique styles of leadership. The Seven Transformations of Leadership tells us about different styles of leading. Though we may learn what an effective leader does‚ it is ultimately up to the person and his or her personality to really define their leadership style. I found it comforting to see that David Rooke
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The birth of Musa Prophet Musa was born at the time when phiroun was ruling. Phiroun hated the Bani Israel the descendants of Prophet Ya’qoub or Jacob. Phiroun hated and disrespected the Bani Israel. They were kept in places where they keep slaves‚ and forced to work for him for small wages or nothing. The pharaoh wanted the people to obey him only‚ and to believe in the gods of his invention. One day he was told that a child from the tribe of Bani Israel would be born and overthrow him. He then
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The Birth of Amul The Kaira District Cooperative Milk Producers’ Union Limited was registered on December 14‚ 1946 as a response to exploitation of marginal milk producers by traders or agents of existing dairies in the small town named Anand (in Kaira District of Gujarat). Milk Producers had to travel long distances to deliver milk to the only dairy‚ the Polson Dairy in Anand. Often milk went sour as producers had to physically carry the milk in individual containers‚ especially in the summer
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F. The Birth of Venus was created on canvas with the use of tempera paint. Botticelli would hand grind a material that changes according to the light that it reflects‚ also known as pigment. The powdered pigments are traditionally mixed with egg yolk which dries quickly and stays firmly in place (Saitzyk‚ 245). During the Renaissance‚ fabric such as canvas were used instead of the popular wooden panel. As tempera
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Causes and Effects” In this paper‚ I am going to focus on a couple high risk topics that can cause birth defects in an unborn child. I will discuss the effects of smoking‚ alcohol consumption‚ illicit drugs and prescription drug use. Letting people know more of the causes and outcomes of these examples is important because children are always our future and if birth defects and death can be prevented‚ should we not present hardship to them even before their first breath. It is important
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As the birth rates in recent years have been declined rapidly‚ the social issues that associate with older people’s social care come in quick succession. It is important to find out the way in solving these severe problems instead of ignoring. Therefore‚ this essay would analyze the approaches that different countries used and obtain the most sustainable way in order to achieve the social balance. Before comparing the elderlies’ social care approaches in different countries‚ providing the backgrounds
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Bacterial Transformation Lab Introduction: In this experiment we transformed a strain of E. Coli bacteria without antibiotic resistance with plasmid DNA. This plasmid produces a fluorescent green glow under black light due to the gfp(green fluorescent protein) as well as antibiotic resistance. E. Coli cells will be plated on an agar medium‚ some with and some without the antibiotic ampicillin. Only bacterial cells that contain the plasmid will survive the ampicillin and produce the green glow
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