Expressing and Purifying the Recombinant form of Green Fluorescent Protein (rGFP) from the E.coli strain using Ni2+ agarose affinity chromatography technology Abstract The purpose of this experiment was to express and purify the his6-tagged recombinant form of GFP (rGFP) from the organism E.coli using Ni2+ agarose affinity chromatography. The expression of rGFP was confirmed qualitatively using the UV light and was expressed in the E.coli strain BL21 (DE3) (-- removed HTML --) (-- removed HTML
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ATP synthase (EC 3.6.3.14) is an important enzyme that provides energy for the cell to use through the synthesis of adenosine triphosphate (ATP). ATP is the most commonly used "energy currency" of cells from most organisms. It is formed from adenosine diphosphate (ADP) and inorganic phosphate (Pi)‚ and needs energy. The overall reaction sequence is: ADP + Pi → ATP‚ where ADP and Pi are joined together by ATPsynthase Energy is often released in the form of protium or H+‚ moving down an electrochemical
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Thesis Statement This is my thesis statement -- while American animation and Japanese animation both have their virtues‚ the style of American animation‚ in general‚ has a significant amount of higher quality. Where to Begin? Where to be Going? To begin with‚ one of the major problems that has hindered American animation is budget and time constraints. On the other hand‚ in Japan‚ anime has been allowed to flourish all over. When it comes to animation‚ it seems that Hollywood simply does
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Maruti Suzuki has two manufacturing facilities in India. Both manufacturing facilities have a combined production capacity of 14‚50‚000 vehicles annually. During a recent meeting of the Gujarat chief minister with Suzuki Motor Corp chairman & CEO Osamu Suzuki‚ the Chairman had said that the work on car manufacturing plant at Mandal near Ahmadabad would be started soon. GURGAON MANUFACTURING FACILITY:-
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Declaration: I declare that this project is the result of my own individual efforts and that it conforms to university‚ departmental and course regulations regarding cheating and plagiarism. No material contained within this study has been used in any other submission‚ by the author‚ for an academic award….. Pulkit Sharma March 25‚ 2010 [H-16062] Acknowledgement: I would like to thank my module leader M.R
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Marketing Plan Project: Sony Playstation 3 Planned Designed for (Present- End of 2013) MKTG-3103-01 Fall Semester 2012 Submitted to: Richard Brand FVSU Executive Summary This marketing plan is over the Sony Playstation 3 (PS3). The Playstation 3 is a six year old video game system‚ and one of the top 3 gaming consoles along with Nintendo’s Wii and Microsoft’s Xbox. Sony Table of Contents Introduction Situational
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International Journal of Electronics and Computer Science Engineering Available Online at www.ijecse.org 492 ISSN: 2277-1956 3 Factor Securities based on RFID‚ GSM and face Recognition for Visitor Identification 1‚2 Minakshi Gupta 1‚ Ketki Deshmukh 2 Electronics & Telecommunication Department‚ Mukesh Patel School of Technology and Management NMIMS University Bhakti Vedant Swami Marg‚ JVPD Scheme‚ Vile Parle (west) MUMBAI (Maharashtra) Email- 1minakshiagarwal14@gmail.com‚2Ketki.deshmukh@yahoo
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FDT 4 Task 2 Part C Concepts Map Japanese Language Japanese Family Life Parents Extended Family Holidays ObonLabour Thanksgiving Day Emperor’s Birthday African American Language Ebonics Creole Family Life Parents Extended Family Holidays Black History Month and Martin Luther King Day Kwanzaa South central Lower Michigan is mostly white middle class. The most dominant sub cultures are Japanese and African American. Jackson County has a population of 89% whites‚ 8% African American
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Resource.” Journal of Travel Research 33.51 (1995): n. pag. JTR.SAGEPUB. Web. Feb. 2014 Patterson‚ K. (March‚ 2013). Tourism’s Negative Impact on Native Hawaiians Retrieved from http://mphawaii.tripod.com/Tourism/TourismsNegativeImpact.htm Saito‚ Osamu. “Resource Use and Waste Generation by the Tourism Industry on the Big Island of Hawaii.” Journal of Industrial Ecology 17.4 (2013): n.pag. Web. Feb. 2014 Sheldon‚ Pauline‚ and Sun Yun Park. “An Exploratory Study of Corporate Social Responsibility
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MANGA: IT’S VARIATIONS A TERM PAPER Presented to Ms. Fideliza U. Cojuangco Instructor In Partial Fulfillment Of the Requirements for the Course Eng 12: Writing in the Discipline by Edrian Carlos Yu Molina BSCE 1A MARCH 2013 ACKNOWLEDGMENT The researcher would like to express his sincere gratitude to all the persons who helped in making this research study a success. His earnest appreciation is especially given to: Ms. Fideliza U. Cojuangco‚ his
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