Estimating Osmolarity by Change in Weight Abstract Optimum water content for normal physiological processes in plants is crucial. In order for normal activities to take place‚ the amount of water relative to osmotically active substance (OAS) in cells must be maintained within a reasonable range. The one way to estimate optimum water content is to find osmolarity of plants cells. Osmolarity can be indirectly measure by comparing change in weight and volume when plant cells are incubated in
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Osmosis in Potato Tubers Andrew Dickson Background When a plant cell is bathed in a solution of the same concentration (isotonic) as its intracellular environment‚ its mass and volume remain the same. This is because water enters and leaves the cells at the same rate. There is no net loss or gain of water by osmosis. Samples of cells can be placed in a range of solutions of different concentration. The cells will gain water by osmosis when placed in solutions which are more dilute (hypotonic)
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Determining the Osmolarity of a Potato Mishal Hasan Abstract Students in Biology find it difficult to understand the concept of tonicity and osmolarity in a real time situation. In this investigation‚ several concentrations of sucrose were used to determine the osmolarity of a potato. It was found that the concentration of sucrose was close to 3.6 in the potato used showing that it was isotonic. Anything above caused the potato to gain weight showing that it was a hypotonic solution and anything
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Lab Topic: Movement of Materials Across the Cell Membrane—Potato Tuber Cells Introduction: Osmosis is a form of diffusion in which water diffuses through a selectively permeable membrane from a region of high concentration to a region of lower concentration. This form of diffusion takes place when the molecules in high concentration are too large to move through the membrane. Three factors that determine cell membrane permeability are shape‚ size‚ and polarity. For this experiment‚ it must
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Using Sucrose Solutions to Determine Osmolarity of Potato Tubers Based on Weight By Thomas Pelikan Biology 200A Section 004 Kimberly Schmidt October 2‚ 2012 Abstract: In this experiment we were trying to determine the osmolarity of potato tubers by weighing them before and after incubating them in solutions of sucrose with varying molarities. To find the osmolarity we took a potato and used a cork borer to obtain seven samples of potato tubers. We then prepared seven beakers with concentrations
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Measuring the Water Status of Potato Tubers Objectives: Upon completion of this laboratory you should be able to: 1. determine the water potential of a plant tissue by the Chardokov and gravimetric techniques and understand the underlying theory. 2. determine the osmotic potential of a plant extract by the freezing point depression method and understand the underlying theory. 3. describe techniques for measuring the pressure potential of a plant tissue. 4. describe techniques for measuring
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an experiment from which I should be able to draw a firm conclusion that will either prove or disprove any predictions I make. This essay aims to assess and investigate the effect of various solution concentrations on the activity of osmosis in plant tissue. Background scientific theory: Plants exchange gases (CO2 and O2) in maintaining vital respiratory processes and in carrying out photosynthesis; they absorb certain minerals and sugars so to use as a source of energy and eradicate wastes in
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Unit 4 Lab – Effects of Osmosis on Living Tissue Assignment Total: 35 Points Course Weight: 6 Pre-Lab Preparation - Review the following resources to complete this assignment: Textbook: Chapter 5‚ pg. 83-86 Mastering Biology / Study Area / Chapter 05 – The Working Cell / Student Home Key Concepts Video: Plasmolysis Video: Turgid Elodea Activity: Membrane Structure Activity: Diffusion Activity: Osmosis and Water Balance in Cells Bioflix: Membrane Transport Additional Resources Blast Animation: Diffusion
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Experiment: Turgor in Potato Tissue 1. We labelled three test tubes A‚ B‚ C‚ D and added initials of people in our group. 2. Used a syringe or graduated pipette to put 20 cm3water in tube A and 10 cm3 in tube B. 3. Put 20 cm3 of 17% solution in tube C and 10 cm3 in tube B; put 20 cm3 pure sucrose solutions in tube D. 4. Working on the dissecting board I cut the ends off large potato. I was careful with the knife and cut it onto the dissecting board away from myself. 5. Used a cork
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Osmosis in Potato Tissue Experiment Background Information Osmosis can be defined as the movement of water across a semi-permeable membrane from a region of high water concentration to a region of low water concentration. The semi-permeable membrane allows small particles through it but does not allow large particles such as sodium chloride. Osmosis will continue until a state of equilibrium is reached i.e. there is no area with a higher or lower concentration than another area
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