Activity One: 1. The two major variables that affect the rate of diffusion: a. The composition of the lipid bilayer (eg. more cholesterol‚ less permeability to polar substances) b. The structure of the molecule undergoing diffusion (eg. steric conformation‚ size‚ polarity‚ amount and strength of hydrogen bonding) 2. Urea was not able to diffuse through the 20 MWCO because the pores of the membrane were too small for the urea to pass through. The molecular weight of urea is 60.06 g/mol‚ over
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Transport Hands-On Labs‚ Inc. Version 42-0034-00-01 Lab Report Assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a su Premium 609 Words 3 Pages Diffusion and Osmosis of Solutes and Water Across a Membrane Diffusion and Osmosis of Solutes and Water Across a Membrane Brittany Bacallao Nova Southeastern University Abstract: This experiment gave a visual understanding of osmosis and diffusion. The first experiment
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Osmosis Lab: The Incredible Egg How does the solution a cell is in effect the movement of substances into and out of the cell? Eggs were soaked in vinegar for twenty-four hours before the lab to dissolve the shell‚ then placed them into the distilled water. Then the eggs were taken out and dried by the paper towel to measure their weight on the electronic scale. First egg weighted 78.92g‚ most of the parts of it’s skin was white translucent‚ and was spongy. After the egg was submerged into the
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DIFFUSION AND OSMOSIS Chapter 3 of your textbook explains diffusion and osmosis. Diffusion is simply the net movement of atoms or molecules from a region of higher concentration to a region of lower concentration. The force behind the movement is heat or kinetic energy (also called Brownian motion). Diffusion occurs when you spill water on the carpet floor and it spreads out‚ or when you open a bottle of perfume and it leaves the bottle and spreads throughout the air in the room. Osmosis is a
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Advanced Placement Biology ® AP Biology Lab 1 281 EDVO-Kit # Storage: Principles & Practice of Diffusion & Osmosis Store entire experiment at room temperature. EXPERIMENT OBJECTIVE The objective of this experiment is to develop an understanding of the molecular basis of diffusion and osmosis and its physiological importance. Students will analyze how solute size and concentration affect diffusion across semi-permeable membranes and how these processes affect water potential. Students
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Hypothesis - If the glucose and starch solution stays within the beaker of water for a longer period of time‚ then more water will travel through the selectively permeable membrane due to the effects of diffusion on the solution. If the level of sucrose within the solution increases‚ then the effects of osmosis will cause the change in mass to also increase due to higher concentrations of sucrose and the increased net movement of the water molecules within the solution. Analysis - When the glucose and
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Retrieved October 29‚ 2013‚ from http://prezi.com/wii0fgw48euc/potatoe-enzyme-lab/ What enzyme is present in liver and potatoes. (n.d.). Retrieved October 29‚ 2013‚ from http://wiki.answers.com/Q/What_enzyme_is_present_in_liver_and_potatoes#slide2 What Is the Optimum PH for Catalase - Ask.com. (n.d.). Retrieved October 29‚ 2013‚ from http://www.ask.com/question/what-is-the-optimum-ph-for-catalase What is the optimum PH for potato catalase and why? - Yahoo! Answers. (n.d.). Retrieved October 29‚ 2013
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The Effect of pH on Enzyme Activity A piece of Solanum tuberosum (potato) was removed and mixed with distilled water in a blender. The resulting solution was filtered through multiple layers of cheese cloth to filter out the liquid by eliminating any large pieces in the solution. The solution created was catechol. Five different solutions were prepared as blanks with each test tube containing 6.0mL of a different pH (pH 4‚ pH6‚ pH7‚ pH8‚ pH10) of phosphate buffer‚ 1.0mL of the enzyme and 1.0mL of
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which came from the potato juice. The function of an enzyme is to catalyze reactions in cells. The specific function of the enzyme in this experiment was to see if it affected the activity if it was exposed to different temperatures. A substrate is a material or substance which the enzyme acts. The substrate in our lab was hydrogen peroxide. Procedure For our experiment we used‚ three test tubes‚ nine milliliters of potato
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3%‚ was diluted using 10 mL of hydrogen peroxide and 20 mL of water. In the second beaker‚ a ¼ dilution of potato extract was made using 2 mL of potato extract and 6 mL of water. The third plastic beaker contained 8 mL of water. Using the first and second beaker the experimental assay was performed. Using forceps a small paper disk was dipped into the second beaker containing the potato catalase for exactly 5 seconds. It was next dried on a paper towel for 5 seconds‚ and finally placed at the
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