Title: DNA analysis Aim: a) Isolate and Purify Bacterial Chromosomal DNA from a strain of E.coli b) Visualization of restriction fragments by Agarose Gel electrophoresis Objectives: * to isolate and purify bacterial chromosomal DNA from a strain of E.coli * to analyze and identify DNA by use of a spectro-photometer * to use restriction enzymes to cleave DNA into fragments * to visualize the restriction fragments by gel electrophoresis * to compare the different
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temperature)‚ and 70°C. This investigation tested the hypothesis: The rate of osmosis will increase as the temperature increases 2x2x2cm cubes of potato were weighed and then placed into 100mL of 10% NaCl solutions for 10 minutes. After 10 minutes the cubes were again weighed to determine the amount of mass that they lost/gained and thus get an indication of the rate of osmosis. The results collected showed that the rate of osmosis was highest in the 70°C solution and lowest in the 20°C solution. From
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Gadget Toys Case Write-Up Gadget Toy Company is faced with a choice between two options to reduce project time for their new toy line. Gadget must cut down project time in order to meet their Christmas deadline that is 19 weeks away. Analysis shows that there are two critical paths for the current process‚ which is 20 weeks long and includes the following critical activities: obtain funding approval (A)‚ finalize engineering (B)‚ build dies/tools (D)‚ raw material delivery (K)‚ initial production
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Gone Rural Case Write-Up 11/19/14 1. How would you advise Ms. Thorne concerning growing her organization‚ being financially viable and achieving her mission? In order to grow her organization‚ our group advises that Ms. Thorne invest a total of R1 million in new additional facilities to double the operating capacity of the company. In order to grow the company and expand into foreign markets‚ the company needs to build another facility. It was made clear that the limiting factor in production
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Diffusion is one of several transport phenomena that occur in nature. A distinguishing feature of diffusion is that it results in mixing or mass transport without requiring bulk motion. Thus‚ diffusion should not be confused with convection or advection‚ which are other transport mechanisms that use bulk motion to move particles from one place to another. In Latin‚ "diffundere" means "to spread out". There are two ways to introduce the notion of diffusion: either a phenomenological approach starting
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THE CHEMICAL CONSTITUENTS OF LIVING MATTER Relevant IB topic: T3 Time: 2 hours BACKGROUND The principal chemical constituents of living matter are: water‚ mineral salts‚ organic compounds such as carbohydrates‚ proteins‚ lipids‚ nucleic acids. In this exercise we shall concentrate on carbohydrates‚ lipids‚ proteins‚ and we shall estimate the concentration of Vitamin C‚ an organic compound‚ in a solution‚ by the
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Introduction Osmosis is the movement of water from a region of higher concentration ( hypertonic ) to a region of lower concentration ( hypotonic solution ) through a cell membrane or other semi-permeable membrane until an equilibrium is reached. It is a special case of diffusion called “ passive transport “ which means no energy is required. Diffusion is the movement of a substance by which the molecules move from a region of higher concentration to a region of lower concentration. Aim
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Apple PowerBook Case Write-up After the failed release of Apple’s first miniaturized computer‚ the Portable‚ the company was faced with the likelihood of losing significant ground in the mobile computing market if it didn’t bring a product to market at record speed. Apple had anticipated the Portable would be competitive enough to maintain market share until its longer term “Companion” project was complete. However‚ thirty-six months remained until Companion would be ready. Weak sales coupled
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Crime Scene Write-up CRJS 210 Professor Rinehart August 27‚ 2012 Upon entering the crime scene to my right (North wall)‚ I noticed a white male‚ wearing a gray sweat suit‚ hanging from the ceiling with a small rope around his neck and his hands bound behind his back with plastic zip-ties. In addition‚ there is blood splatter on the wall (North wall) to the right of the victim. The second victim‚ a white male wearing a black ball cap‚ black tee shirt and plaid pants were found semi lying on
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the work she will deliver. Therefore‚ losing future clients and eventually leading to the close of her business. The second alternative of raising money by borrowing‚ whether from a financial institute or from family and friends‚ will definitely pump up the solvency/cash criteria. Which will lead to enhancement in the quality of work delivered‚ due to the fact that that money will allow her to keep all her employees and keep the space she’s renting - providing the employees a stable environment to
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