5-dinitrobenzoic acid to oxidize the aldehyde group on the carbohydrate‚ the reducing end of glucose increases in absorbance of 540 nm. Using a UV spectrophotometer‚ the concentration was calculated by using a regression line of the standard curve. The experimental data concluded a 1068.912 µg/ml. With the actual concentration being 1200.000 µg/ml‚ a 10.924% error was deduced. Though the error is slightly high‚ the deviation of 134.370 µg/ml makes up for some of the error through this process;
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13‚ 2013 Determining the Concentrations of Red Dye in Sodas Abstract The use of red dye #40 is common in various soft drinks today. The labels on these beverages do not specify how much dye we are consuming. We did this experiment to find out which soda uses the most dye. Using a spectrophotometer‚ we measured how much light is absorbed by various known concentrations of red dye. After collecting this data‚ a standard curve was made that correlated the concentration of red dye #40 to its absorbance
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The effect of salt concentrations on the mass of the pear cubes ‘Pyrus’ after being soaked in water Aim: The effect of salt concentrations on the mass of the pear ‘Pyrus’ pieces shaped as cubes of 1cm. Background: Osmosis is the net movement of water molecules through a semi-permeable membrane from an area of higher water potential to an area of lower water potential. Water makes up to 70-90% of living cells and cell membranes are partially permeable membranes. Dissolved substances attract
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Noel Arackal 1.25.16 Bisc 4930 Experiment No. 1 – Varying Extracellular Concentration Introduction: This experiment sets out to determine the tonicity of solutions with varying concentrations of sodium chloride on the red blood cells of sheep by measuring the transmittance of a red blood cell/NaCl solution with a spectrometer. This is done in order to study the effects of solutions containing varying levels of tonicity to red blood cells. Tonicity describes what happens to a cell when it is
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The Effect Of Enzyme Concentration On Enzyme Activity The pancreatic duct in individuals who have cystic fibrosis frequently becomes blocked‚ reducing or preventing the release of pancreatic enzymes into the small intestine. The aim of this activity is to investigate the effect of a reduction in enzyme concentration on the rate of reaction‚ in this case the breakdown of protein by protease enzymes. Aim – Milk powder contains a white protein called casein. A white suspension of
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investigating how the rate of reaction differs when we change the concentration of Hydrochloric Acid whilst reacting with Magnesium. The rate of reaction is explained by the Collision Theory. This theory explains how various factors affect the reaction rates and how chemical reactions occur. The 4 factors of the Collision Theory are: • Temperature • Concentration • Surface area • Catalyst I am investigating how different concentrations of acids affect the rate of reaction. However I must control
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Experiment 2: Estimation of protein concentration Name: Wong Jiun Hao ID: 00000011136 Cohort: BM114 Module: Biological Science Table of Contents Title 1 Table of Contents 2 Introduction 3 Objectives 4 Procedure 4 Results 5-6 Discussion 7-8 Conclusion 9 References 9 Introduction The concentration of protein in a sample can be obtained by using the Bradford protein assay method. The method requires a spectrophotometer
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No; the experiment was not successful‚ because it did not show the correct fluorescence gradation of the serial dilution of the DNA concentration. As shown by the picture‚ only the first drop of the DNA/EtBr mixture for the DNA standards fluoresce brightly under the UV light‚ while the other spots for both the DNA standards and the unknown DNA standards were all dimly fluoresce. This was due to pipetting error; the tip of the pipette did not touch the liquid (TE) in the micro-centrifuge‚ so no DNA
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There are numerous concentration camp that were constructed across Nazi Germany from 1939-1945. During this time period‚ Jews‚ gypsies‚ homosexuals‚ and bountiful of others forced by the Nazi regime to these concentration camps. There were countless experiments performed on those who were in certain camps‚ but mostly forced labor to build items for the German army. Of all of these camps one was the most notorious‚ which was Auschwitz concentration camps. This camp was the largest of its kind‚ but
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Minimum Inhibitory Concentration (MIC) is a way to measure the sensitivity of microbes to antibiotics or antifungals (antimicrobials). Organisms are tested for growth in various concentrations of antibiotic. This allows microbiologists to see which concentration (MIC) of antimicrobial will be effective against the pathogen. It also used for determining treatment for patients suffering from infections such as pneumonia‚ meningitis‚ endocarditis or osteomyelitis or managing the treatment of high-risk
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