Techniques for Isolation of Pure Cultures Objective : i. To perform the spread plate and the streak plate inoculation procedure for the separation of the cells of a mixed culture so that discrete colonies can be isolated. ii. To prepare a stock culture of an organism using isolates from the mixed cultures prepared on the agar streak-plate and/or the spread plate. Introduction : In order to be able to adequately study and characterize a certain microorganism‚ microbiologists need to separate
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What happens when you make a baby monkey choose between food and comfort? The Harlows answered this question in a series of primate experiments. Love is important‚ so how will these lonely monkeys function without it? Though the Beatles confidently tell us that ’all you need is love‚’ behavioral psychologists were skeptical that people and animals need--or are motivated by--anything other than food‚ water‚ shelter and sex. Psychologists Harry and Margaret Harlow decided to determine scientifically
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Anthony Young Partner: Nick Dale TA: Alehkya 6/8/15 Isolation of Trimyristin from Nutmeg and Preparation of Myristic Acid from Trimyristin by Hydrolysis. Introduction In this experiment‚ trimyristin will be isolated from the nutmeg by extraction‚ and then used to prepare myristic acid by hydrolysis. The isolation of a substance from a natural product is a common technique used nowadays. Usually‚ the isolation is a complex procedure and requires a lot of steps and preparation. For this experiment
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Assay of succinate dehydrogenase of after isolation of mitochondria in Cauliflower (Brassica oleracea) using differential centrifugation. Kelly M. Messick‚ Rebecca Conner Department of Biological Sciences‚ Salisbury University‚ Salisbury‚ MD‚ 21801 U.S.A Address for correspondence: Kelly M Messick Department of Biological Sciences Salisbury University Salisbury‚ MD 21801 Phone: 410-546-2060 Fax: 410-543-6433 e-mail: km96536@gulls.salisbury.edu Running title: Assay of succinate dehydrogenase
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Othello racism Othello tragedy Dustin Mills English 102 Dr. Elisabeth Sachs Othello Essay Honest Iago One of the most interesting and exotic characters in the tragic play Othello‚ by William Shakespeare‚ is honest Iago. At first Iago seems to be motiveless. However‚ the motivation behind his actions lie more in Iago’s quest for personal gain‚ as opposed to just being evil for evil’s sake. Iago’s greediness can be validated by examining his manipulation of Roderigo‚ Cassio‚ and most importantly
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Isolation of Bacteria in our Physical Environment Introduction: The purpose of the lab is to apply what has been learned so far in Microbiology‚ and use it to collect and examine microbial species from the physical environment. The physical environment around the lab will be utilized to find out the variety of different microbial species growing in and around the lab. This investigation will help in preparation for the individual projects that will be done at the end of the semester. Materials
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Experiment 1: Isolation of Casein from Milk * pH of milk – 6.6 * milk = 87.1% water‚ 4.9 % CHO 3.9 %‚ fats‚ 0.7% minerals Experiment 2: Protein Hydrolysis and Characterization | Reagents | Principle | Test for | Positive Result | Negative Result | Biuret | CuSO4‚ NaOH | Complexation of Cu+2 with amide N atoms | Polypeptide bonds | Violet/purple solution | Blue color solution | Sakaguchi | 10% NaOH‚ 0.02% α-naphtol solution‚ 2% NaOBr | arginine condenses with α-naphtol and NaOH
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Isolation and identification of an unknown bacterium Pillay‚ Esmerelda (209504371) School of Biochemistry‚ Genetics and Microbiology Department of Microbiology University of Kwa-zulu Natal 25 October 2010 ABSTRACT Different types of bacteria in various forms are found all around us‚ and it is a microbiologist’s job to be able to identify these bacteria. Using various staining techniques and physiological tests‚ an isolated bacterium can be identified. In this experiment‚ a single bacterial
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Isolation of Caffeine in Tea Introduction Today’s world is addicted to coffee and tea for an easy wake up. Both contain caffeine‚ a drug known for its stimulant properties. Tea‚ by weight‚ contains approximately 2-4% caffeine‚ and the average cup of tea has anywhere from 40 to 120 mg of caffeine (Landgrebe‚ 327; Mayo Clinic Staff). According to the American Heart Association‚ caffeine not only stimulates the brain and nervous system‚ but it releases fatty acids from adipose tissue‚ and affects
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Othello Othello is a Shakespearean play and a morality tale‚ which is set in Venice‚ Italy and represents the themes of race‚ death and at its very heart is the ‘green-eyed monster’: Jealousy. The character of Othello is insecure and naïve. His race‚ a major theme in the play‚ shows how Othello perceives himself as a rough outsider‚ though he is nothing of the sort and his skills are nevertheless powerful‚ his power being personified by the fact that he is always surrounded by people. In Act 1
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