In this lab we tested the effect of temperature has on the rate of enzyme activity. The way we figured this out was by taking four different temperatures and testing the difference absorbance levels they produced every 20 seconds for about 2 minutes straight using a spectrophotometer. The important part of this experiment was the temperature the enzyme concentration was made at. What we got from the experiment was at lower temperature we got very low numbers for the absorbance‚ which gave us a lower
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Effect of Temperature on Enzyme Activity Bernard Stepteau Biology Lab 101/Th 8:00am – 9:50am 2-26-2014 Dr Laynes Hypothesis: As the temperature of the enzyme catalase rises the activity of the reaction will decrease. Objectives: The objective is to compare catalase activity at different temperatures. Introduction Catalase speeds the breakdown of hydrogen peroxide. Catalase is present in every plant and animal organ. This is useful because hydrogen peroxide is harmful
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Centripetal Force Lab Activity Analysis: 1. A) Average Percent Difference: 50g: (values expressed in newtons) Step 1: Calculate the average value of the two variables Average Value= Value 1+ Value 2 /2 = 0.49+ 0.61/2 = 1.1/2 = 0.55 Step 2: Calculate the difference between the two variables Difference= Value 2- Value 1 = Fc- Fg = 0.61- 0.49 = 0.12 Step 3: Calculate % difference % difference= difference
Free Energy Potential energy Kinetic energy
Title: Enzyme Activity Lab Purpose: To measure the rate of enzyme activity from a tissue abstract and experiment with different factors‚ such as the enzyme solution and the substrate with different hydrogen peroxide percentages and temperature‚ that affect enzyme activity. Hypothesis: 1) If the disk is placed into each beaker with 100 units/ml of enzyme solution‚ then the time for the disk to float will be 30 seconds. 2) If the temperature of the solution is at 5 degrees Celsius‚
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Activity 1: Simple diffusion Introduction: Simple diffusion is the net movement of substances from a region of high concentration to a region of low concentration so its overall net movement is along the concentration gradient‚ simple diffusion does not require energy therefore it is ’passive’‚ substances are diffused across the membrane between the phospholipids. Materials and methods: * 20 mwco dialysis membrane * 50 mwco dialysis membrane * 100 mwco dialysis membrane * 200 mwco
Free Diffusion Molecular diffusion Osmosis
2/15/2013 background on transformation of bacteria with pGLO plasmid Experiment #5 Aim: Purpose of this lab is to have plasmid activity transformed Material: Bacteria starter plate‚ pGLO DNA Plasmid‚ microcentrifuge tubes‚ Ice‚ water bath‚ CaCl2 Transformation solution‚ (LB) agar plate‚ (LB/Amp) agar plate‚ (LB/Amp/ara) agar plate‚ Micropipette‚ and Micropipette tips. Method: Genetic transformation is a procedure which is done by taking genes from one organism and putting them in another organism
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Factors Affecting Enzyme Activity Abstract: In the following lab factors affecting enzyme activity‚ temperature‚ pH‚ enzyme concentration‚ substrate concentration and surface area will be tested on a beef liver enzyme to see if there will be any effect of performance. By doing 2 or more trials the results will show whether there is an effect to the enzyme from the following factors or not. Some of the factors may denature the enzyme and some will do nothing. Using a table qualitative and the
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LABORATORY REPORT (Click on the Save a Copy button on the panel above to save your report) Activity: Enzyme Activity Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 60 °C (140 °F) 3. Sucrase activity decreases with increasing sucrose concentration. Materials and Methods Effect of pH on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable. pH 3. Controlled Variables
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temperature on the enzyme activity was that the reaction’s rate would increase as the temperature increased‚ until they go over the optimum temperature where the enzymes denature and the reaction’s rate quickly drops to zero. At 5 degree C the rate is 0.00059mole PNP/min. This then increases to 0.01031mmoles PNP/min at a temperature of 50 degree C. The rate then drops drastically to -0.00215moles PNP/min. This point is where the enzymes have been denatured and have no activity‚ shown as the last point
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ASSAYING PLANT SUBSTANCES FOR ANTIBACTERIAL ACTIVITY PURPOSE To test plant extracts to see if they possess antibacterial activity. PROCEDURE First create a list of plant extracts being tested and choose 2 extracts per group. The plants being tested are Oregano‚ Tea tree‚ Eucalyptus and Colloidal Silver. Obtain two nutrient agar plates and using a permanent marker draw four quadrants on the bottom of each agar plate. Using a sterile pipet transfer 250 ml of E. coli broth to the middle of
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