Experiment 5 Title : Thin Layer Chromatography Objectives: i. To distinguish polar and non-polar solvents. ii. To familiar with the analysis technique by using the thin layer chromatography. iii. To differentiate the retention factor‚ Rf for different compounds. [pic] Result: |Compound |Distance traveled by the compound | |o-nitroanaline |2.45
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the slurry to be placed on the glass plates. A guide allows the applicator (with an adjustable layer thickness gauge) to be pulled along the glass plates as the slurry is dispensed. Some practice is needed to get a continuous body motion pulling the applicator across the plates to get an even layer. The glass plates must be the same thickness and extremely clean. Any oil or grease will result in a poor layer. Handle the plates with rubber gloves when washing or rinsing. Care is also needed if the edges
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Thin Layer Chromatography Submitted in partial fulfillment of the requirements for: CHE 324 Organic Chemistry Laboratory Dr. Robert Duncan Fall Semester 2012 Caitlin Inman‚ Team Leader October 9‚ 2012 Tyler Byrd‚ Data Collection Shared Role‚ Technique Expert Introduction: “Chromatography is used to separate components of a mixture. For example‚ imagine a mixture of wood pieces‚ pebbles‚ and large rocks to be separated and the chromatography setup as a stream. Flowing water
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Jaslyn Low Foon Siang CFD Assignment: Laminar Boundary Layer Form a group of 4-5 students to run a CFD simulation in laminar flow to answer the following questions. You can use any geometry with proper dimensions and make necessary assumptions in this simulation. Questions a) Show the developing and developed flow patterns. b) Show the inviscid and laminar flow patterns. c) Show the growth of the boundary layers. d) Compare the boundary layer thicknesses with Blasius solution. e) Show the centerline
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It was concluded that the product created was paracetamol when a thin layer chromatography test was performed and observed under ultraviolet light (which is discussed further down the page). An unknown substance was treated with acetic anhydrate and resulted with paracetamol. A compound that behaves in this matter is 4-aminophenol and is widespread in the industrial production of this drug. Upon these observations was the build up of “Chemical A” decided. When 4-aminophenol is t treated with
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The 7 layers can be split logically into two subgroups. Layers 7 thru 4 focus on the end to end communication of data source and destinations. Layers 3 thru 1 are provide consistent communication between the network devices. An easier way of looking at the OSI model is dividing the upper layers (7‚ 6‚ 5) from the lower layers (4‚ 3‚ 2‚ 1). The upper layers deal with application issues and are implemented only in software. The highest layer‚ the application layer‚ is the closest to the end user
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Checkpoint: OSI Model Paul Tegner IT/242 April 22‚ 2011 Mark Burke OSI Model The OSI Model consists of seven layers. These layers are the application layer‚ presentation layer‚ session layer‚ transport layer‚ network layer‚ data link layer‚ and physical layer. The Application Layer The application layer is responsible for interaction between the operating system and the network services and provides an interface to the system. It provides the user interface to a range
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Cfcs Cause Deterioration of the Ozone Layer The deterioration of the ozone layer ‚ caused by Cfcs‚ endangers the lives of humans ’. Cfcs have a diminishing effect on the ozone layer. Furthermore‚ the deterioration of the ozone cause an increase of Ultraviolet (UV) radiation which can have a negative effect on human skin and eyes. As a writer for newsweek‚ I have investigated the scenario and found the following information. The earth ’s atmosphere is a blanket of air that surrounds the planet
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makes it a useful solvent for many chemical processes. The solid was already in powder form therefore we did not have to crush it but it the unknown was in a tablet form‚ one would need to reduce it to a powdery-like form in order to perform this Thin-Layer Chromatography experiment. The powder solution was thoroughly mixed with a glass rod in the test tube containing
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Date:7.6.2013 Objectives: Part 1: (i)To learn the technique of TLC and the visualization of colourless components. (ii)Identify the unknown drug by a TCL comparison with standard components. Part2 :to learn the separation technique by using TLC plate in separating a mixture of compound by using spinach leaf. Apparatus:UV lamp‚capillary ‚250 ML beaker Materials:aspirin‚acetaminophen‚caffeine‚unknown A‚unknown B‚TLC plates‚ethyl acetate‚Hexane‚acetic acid‚iodine Introduction: Procedure:
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