EXPERIMENT 8 THIN LAYER CHROMATOGRAPHY AND MELTING POINT DETERMINATION: DETECTION OF CAFFEINE IN VARIOUS SAMPLES Additional Resources http://orgchem.colorado.edu/hndbksupport/TLC/TLC.html http://coffeefaq.com/caffaq.html Materials Needed TLC equipment: 1 5-cm x 8-cm TLC plate‚ 2 capillary micropipettes‚ TLC tank and lid‚ ruler‚ UV lamp Extraction Equipment: spatula‚ 2 small test tubes‚ Pasteur pipet and bulb Chemicals: caffeine‚ ethanol‚ dichloromethane‚ TLC solvent (5% acetic acid in ethyl acetate)
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Purpose 1. To prepare a dry-packed sample of product mixture of ferrocene and Acetylferrocene 2. To separate and purify the components in the product mixture by column chromatography. 3. To check the purity of the components by thin-layer chromatography (TLC). 4. To calculate the yield of acetylferrocene and the percent recovery of unreacted ferrocene. Table of Quantity showing various physical properties Type of substance Molecular Formula Molecular Weight (g/mol) Density(g/cm3) M.P
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descended to just above the sand layer. This yellow band later developed to about 2 centimetres long‚ and moved downwards almost as quickly as the eluent (hexane). This band was possibly beta carotene‚ as beta carotene is a non-polar molecule‚ and interacts well with the non-polar solvent‚ but poorly with the polar static phase (SiO2). The yellow band was collected and labelled S2. No colour band was seen above this yellow band‚ but in the meantime‚ a dark green layer was seen attached to the top of
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Janna Vernice R. Villalon*‚ Christian V. Villanueva‚ Cyd Vincent L. Zamora Department of Chemistry‚ College of Science *Corresponding author; e-mail: janna.villalon@rocketmail.com Abstract In analyzing the chromatography of analgesics‚ thin layer chromatography (TLC) was used. A very thin (micron) film of silica is coated on a glass plate or a sheet of aluminum or plastic. The ingredients of commercial analgesics were characterized in comparison with chromatograms of aspirin‚ Acetaminophen
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Assignment in Computer Science 1 1. IMAP (Internet Message Access Protocol) -is an application layer that allows an e-mail client to access e-mail on a remote mail server. It supports both on-line & off-line modes of operation. The current version‚ IMAP version 4 revision 1 (IMAP4rev1)‚ is defined by RFC 3501. 2. POP (Post Office Protocol) -is an application layer Internet standard protocol used by local e-mail clients to retrieve e-mail clients to retrieve e-mail from a remote
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| | | No more than one bad frame per 105 bytes of data. | ____________________ depicts routers‚ logical links between routers‚ and high-level routing protocol configuration information. Answer | | The Layer 2 STP domain | | | A data link layer device map | | | The Layer 3 topology of an internetwork | | | A logical map | Most systems follow a cyclical set of phases: Answer | | start-up‚ iterations‚ feedback‚ final release. | | | initiation‚ assessment‚ completion
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XYZ NETWORK DESIGN XYZ Network Design Franklin Joel Grimes IT 230 Executive Summary This paper will help to clearly define the network design of XYZ Corporation by ABC Computing. Together we will meet your IT challenges in a seamless‚ highly organized fashion. What we must address together‚ are the following: ⎫ What are the goals of the network design project ⎫ What things may be assumed ⎫ The pros and cons of our proposed IT solutions ⎫ The exact
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4. Ibuprofen (C13hH18O2) Introduction In this experiment‚ several analgesics were analyzed by Thin Layer Chromatography (TLC) and the composition of an unknown tablet was identified. We define chromatography as the separation of two or more compounds or ions by their molecular interactions by either a moving or a stationary phase.1 There are different types of chromatography: Thin Layer Chromatography (TLC)‚ Gas Liquid Chromatography (GC)‚ and Column Chromatography (CC). All of which there
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pigments were extracted by using column chromography and alumina was used as the solvent. Solvents of different polarities were used‚ starting with the least polar‚ to extract the certain components from the leaves. They were then analyzed by using thin- layer chromatography. Procedure: The first part of the experiment dealt with breaking down the spinach leaves in a mortar and pestle. Acetone was added to this to help with the breakdown of the spinach leaves. Once the spinach leaves were broke down
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Query Parsing At the Application Layer to Prevent Sql Injection Presented by Saurabh Jain --------------------------------------------------------------------------------------------------------------------- Abstract SQL injection is a code injection technique that exploits a security vulnerability occurring in the database layer of an application. Thus when user input is either incorrectly filtered for string literal escape characters embedded in SQL statements or user input is not strongly
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