This experiment is to determine the unknown DNA plasmid using restriction enzymes and conducting electrophoresis finally comparing the resulting fragments with the known restriction map. In this lab‚ it succeeds in showing the fragments. In this report we will discuss the‚ results‚ limitations and possible errors. Introduction In biology restriction enzymes are used in several ways to modify and manipulate DNA molecules. One common use is to compare pieces of DNA from one that is unknown‚ with fragments
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The First Amendment is the cornerstone of American democracy and the primary protector of the freedoms Americans enjoy. While some might argue that there should be no restrictions on the First Amendment‚ the First Amendment should be restricted in order to promote public safety and ensure the smooth functioning of society‚ and those limitations should be narrowly crafted and explicitly defined by the Supreme Court. A person has the right to voice his or her opinion on a matter‚ regardless of what
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BIO 219 Group 1 Section 66 October 19‚ 2012 The extraction of purified DNA from A. fischeri by restriction digestion using Sal I enzyme and pGEM for shotgun cloning Introduction: The ultimate goal of this experiment is to isolate the lux operon‚ a targeted piece of DNA that causes bioluminescence‚ from Aliivibrio fischeri and insert it into the DNA of Escherichia coli in order to make it glow. A. fischeri is a gram-negative bacteria which participates in a symbiotic relationship with
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necessary to identify the vehicles that run on the road. So Automatic Number Plate Recognition (ANPR) System was developed. Automatic Number Plate Recognition (ANPR) System is essential in Intelligent Traffic Systems. ANPR Systems are made up of high-speed cameras designed to capture a photograph of each and every passing license plate‚ combined with software that analyses those photographs to identify the license plate number. These systems store extensive location information about each automobile
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In the early 1960s‚ the emergence of the theory of plate tectonics started a revolution in the earth sciences. Since then‚ scientists have verified and refined this theory‚ and now have a much better understanding of how our planet has been shaped by plate-tectonic processes. We now know that‚ directly or indirectly‚ plate tectonics influences nearly all geologic processes‚ past and present. Indeed‚ the notion that the entire Earth’s surface is continually shifting has profoundly changed the way
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How has your understanding of Letter Two reshaped your understanding of marriage and female identity in Pride and Prejudice? In Weldon’s novel‚ Letters to Alice‚ she scrutinises and exposes the reality that women faced in the Regency period in regards to marriage and female identity. This forces the reader to rethink their prior views of women that were shaped by Austen’s context‚ thus appreciating her novel on a deeper level. In comparison to Austen’s text which emphasises the necessity for Georgian
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| | |Plate Tectonics Press Release | | | |
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In today’s society there seems to be many different restrictions in place. Many people would say too many. For example‚ restrictions on the minimum age requirement before you are allowed to drive a vehicle‚ minimum height requirements for children and adults in order to be able to ride a roller-coaster‚ and how old you have to be in order to legally be allowed to consume alcoholic beverages. Although‚ many of these restrictions are in place to ensure the safety of not only ourselves‚ but
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However‚ the First World War forced them to scrap this policy and increase the restrictions placed on civilians in order to successfully fight the war. They did this in a number of ways‚ and this answer will include: Conscription‚ Censorship‚ restrictions on women and food rationing. It will be argued that the most extensive increase in state control was food rationing. The most extensive increase in restrictions on civilians was rationing of food. Food had to be rationed due to the fact that
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Biology Assessment: Influenza Structure: Viruses are non-cellular obligate intracellular parasites‚ requiring a living host cell in order to reproduce. A developed viral particle (virion) lacks the metabolic machinery of cells‚ containing just a single type of nucleic acid (DNA or RNA) encased in a protein coat or capsid. Viruses can be distinguished by their structure and by the nature of their genetic material (single or double stranded DNA or RNA). Viruses that affect humans are more difficult
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