the 1X TBE buffer. The surface of the gel should be completely submerged. Finally‚ we removed the gel comb and added the sample. Thirty micro meters of each sample was placed in the wells according to the following order: Lane Tube Description 1 Markers Standard DNA Fragments 2 CS1 DNA from crime scene cut with enzyme 1 3 CS2 DNA from crime scene cut with enzyme 2 4 1 DNA from suspect 1 cut with enzyme 1
Premium DNA Molecular biology Protein
Entry on conversation analysis This entry attempts to analyze a conversation between a teacher and two students in the classroom. The key issue will be discussed is turn-taking‚ especially overlap and interruption when speakers take turns. The following episode was chosen from a textbook which is now used for discourse analysis. The conversation was produced by a lecture and two students in a university tutorial. The lecturer asked student Kylie and Tadashi about certain topics which
Free Madrid Metro Metropolitana di Napoli
continue to develop your communicative competence in the target language and to understand the target culture. Required Materials: Suggested Materials: . Notebook . Spanish/English‚ English/Spanish Dictionary . Pencils/ Pen . Color Markers . Three ring binder . Construction Paper . Index cards . Scissors‚ glue‚ and ruler Classroom Expectations: Students are expected to conduct themselves in a respectful manner. Every student is expected to be prepared to come to class
Premium Spanish language Education Language acquisition
morality of crossing species gene‚ next‚ is the food safety issue. Plants are the basis of human diet. A major worry of the public is that genetic modified plants contain ethically sensitive genes‚ for example‚ antibiotic marker genes. The obvious fear is that antibiotic marker gens could be recruited into humans rendering antibiotics ineffective in curing bacterial
Premium Genetically modified food Genetically modified organism DNA
Animal Intelligence In the beginning of “The Most Dangerous Game” Rainsford stated “Who cares how a jaguar feels?....They’ve no understanding.” “The Most Dangerous Game” is a short story about how a character believes that animals do not have feelings‚ just when he‚ ironically‚ gets hunted himself‚ but by a human. The author‚ Richard Connell‚ has written various American short stories for all ages. Contrary to Rainsford’s statement‚ I believe that all animals can reason. If humans cannot understand
Premium Human Sign language Thought
ChickaChicka Boom Boom Jaclyn Gosciak Early Childhood Curriculum CE215 Kaplan University Misty Kimbrell Activity 1 Activity name: Putting Letters on the Top of the Coconut Tree Age group: 3-4 Objective(s): They will be working on letter identification and formation. Developmental domain addressed: By the children working together‚ they will be working on communication by having to recognize what letter goes where and social/emotional by working with each other to make the tree. This
Premium Typography Orthography Water
1.1 Types of Inventories Inventory is a list of goods and materials‚ or those goods and materials themselves‚ held available in stock by a business. 1) The type of inventories using by Padini Holdings Berhad is categorised under “Goods for Sale”. For example‚ cotton‚ polyester‚ viscose‚ fibre‚ yarn and et cetera. For “Goods for Sales” inventory is usually divided into 1. Raw Materials : a) Natural Raw Materials: Cotton‚ Wools and etc. b) Synthetic Raw Materials: Acrylic‚ Viscose‚ Nylon
Premium Supply chain management terms Textile Yarn
Multiplication Facts 0 - 12 worksheet A Name_____________________________ Date _____________ Score ______/100 Minute Marker 1 2 3 4 5 Multiplication Facts 0 - 12 Five minute timed drill with 100 problems. 4 × 4 6 × 3 7 × 4 0 × 0 2 × 2 7 × 1 5 × 3 2 × 1 10 × 7 9 × 1 8 × 0 12 × 6 11 × 5 10 × 8 3 × 1 11 × 9 5 × 2 3 × 3 12 × 4 10 × 1 10 × 10 12 × 0 10 × 2 9 × 7 11 × 8 4 × 3 10 × 5
Premium Harshad number
-1Observer: Michelle Stanley Date of Observation: 3/2/10 Fictitious Name of Child: Abby Setting: Bed room and living room Child’s Age: 3 years and 10 months Physical Description of Child: When I walked into the house Abby was wearing her princess Belle dress-up gown. Her hair is bright blonde‚ down l loose and knotted‚ her eyed are blue‚ her skin is pale and her cheeks are rosy. Time Observed: 4:00pm - 4:10pm Behavior Observed: Upon entering the house Abby politely
Premium Median Developmental psychology The Age
1. The final yield of the cells can be determined by use of an automated cell counter or by use of a hemocytometer (Neubauer chamber). In our laboratory‚ we determine the number of purified cells in a Neubauer-improved‚ bright line chamber with V-slash with a depth of 0.1 mm and a counting area of 0.0025 mm2 from Marienfeld (Lauda Königshofen‚ Germany‚ PN # 0650030). 2. For determination of the final cell yield‚ mix a 10 µl aliquot of the purified cells (Figure 9D) with 10 µl of trypan blue solution
Premium Natural number Left-wing politics Sample size