DNA extraction lab 1. A number of steps are required to isolate DNA from cellular content. Describe what happens at each step‚ and why it acts to separate the parts of the cell. The steps include a) breaking cell open to release the DNA; b) separating the DNA from cellar materials and proteins; c) using alcohol to precipitate the DNA; d) cleaning the DNA; e) confirming the presence of the DNA. a) Breaking cell open to release the DNA: the cells are separated from each other by physical means such
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Extraction of Caffeine from Tea Leaves Introduction Caffeine is soluble in boiling water and as a result it is easily extracted from tea bags by steeping in hot water. This process leaves behind the water insoluble portions of the tea bag. However‚ water extracts more than just caffeine‚ so a final separation is done with an organic solvent that will dissolve primarily caffeine. The organic solvent used in this experiment is Dichloromethane (CH₂Cl₂). Dichloromethane is less polar than water
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H83 CEL – CHEMICAL ENGINEERING LABORATORY PROJECT TITLE: SOLID LIQUID EXTRACTION OF GANODERMA LUCIDUM NAME: WIJETUNGE MUDALIGE DINITHI ISHARA WIJETUNGE ID NO: 007118 SUPERVISOR: DR SIVAKUMAR MANICKAM GROUP MEMBERS: KOW NIEN WEI AAZRA OUMAYYAH PANKAN JASON HEW YIP SENG DATE: 30 APRIL 2012 H83 CEL Chemical Engineering Laboratory Solid Liquid Extraction of Ganoderma Lucidum Author: W.M.Dinithi Ishara Wijetunge I certify that: (i) Apart from the experimental results reported in the
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DNA EXTRACTION FROM KIWI ISGR SCIENCE Aalah Yousif‚ Ella Sobek April 27‚ 2012 Background All living organisms (plants‚ animals‚ and bacteria) are made up of cells. Any cell consists of many parts but the parts that play the key roles are the nucleus‚ cell membrane‚ and cytoplasm. The nucleus is like the “brain” of the cell (that also contains DNA). The cell membrane is like a wall that guards the cell. And the cytoplasm is a jelly-like substance which contains other smaller organelles like
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EXTRACTION OF DNA FROM ONIONS ABSTRACT The purpose of the experiment was to experience firsthand the isolation of DNA form a plant tissue without destroying its structure and sequence. A white onion was used for the experiment. After several processes‚ DNA isolate was the visible result. Different chemical tests were performed on the DNA isolate‚ namely: Dische Test‚ Murexide Test‚ Wheeler-Johnson test and Test for Phosphate. Visible results were then noted. INTRODUCTION DNA (deoxyribonucleic
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Chemistry IA How does the molecular mass of alcohols affect its rate of evaporation? Introduction Alcohols are some of the most important molecules in organic chemistry. They can be converted into many different types of compounds. Alcohols contain the hydroxyl functional group (-OH)‚ bonded to a carbon atom of an alkyl or substituted alkyl group. The weight of the different compounds will differs because of the amount of hydrogen bonding. There are three kind of alcohols‚ primary‚ secondary
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Liquid CO2 Extraction of D-Limonene This convenient‚ effective and inexpensive liquid CO2-based extraction of a natural product (D-limonene) provides an engaging example of natural products extraction using an alternative solvent (liquefied CO2). Because the procedure can be carried out in a polypropylene centrifuge tube and uses crushed dry ice as its CO2 source‚ it provides an inexpensive alternative to the equipment-intensive extraction with supercritical CO2. A short video clip is available
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much weaker. Besides strength of the brew‚ growing conditions‚ processing techniques- and other variables also affect caffeine content. Certain types of tea may contain somewhat more caffeine than other teas. II. Methodology Firstly‚ the extraction must be considered. Three tea bags were opened and the combined weights of the tea leaves were taken. Recording the weight is necessary. The tea leaves were returned in the bags. The next step was boiling of the tea bags in 100 mL distilled water
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SEGi University Extraction of Benzoic Acid by Balvinder Singh (SCM 015 677) Lab Partners: 1) Surrean Rao 2) Wong Kai Jun 3) Kanaga Luckshimi 4) Sasiskala Regal ECE2332 Chemical Engineering Practices Submitted: 1st October 2013 Supervisor: Mr Ayman William 1.0 Abstract /summary The purpose of this experiment to carry out in the lab is because to determined the capability to water droplet to carry out the liquid-liquid extraction. The studies is to determine
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DNA Extraction Lab Purpose: To compare the amount of DNA extracted from two different species‚ despite using the same method. Hypothesis: I predict that the liver will produce a higher quantity of DNA than the strawberry. This is because I believe that animals have a higher DNA yield because our structure is more complex than a plant’s structure. Materials: -Sample of Strawberries -Zip lock bag -DNA extraction buffer -Cold ethanol -Glass rod -Double ply cheese cloth -Two test tubes
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