Production of carrageenan‚ particularly κ-carrageenan can be done in either of two ways. The first method‚ which is considered as the original one‚ involves the extraction of carrageenan into an aqueous solution‚ filtration to remove the seaweed residue and finally‚ the recovery of the carrageenan from the solution. Hence‚ product of this extraction is called refined or filtered carrageenan. In the second method‚ carrageenan is not actually extracted but rather‚ everything soluble in alkali and water is
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3.2.4 Genomic DNA extraction of the root tips After the treatment of the onion bulbs with lake water sample at different period of time‚ the DNA from the root tips will be ready to be extracted and observed. First of all‚ the genomic of the Allium cepa will be easily done by the already available Uneasy® Plant Mini kit (Qiagen‚ Germany) in the laboratory. All of the procedures can be performed by referring to the manual provided by the kit. A very small amount of root tips that weigh 100 mg will
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DNA Extraction Lab 1/17/14 Purpose : This lab was conducted in order to show and analyze the way DNA is extracted. Hypothesis: If the lab is conducted properly then we should be able to view a visible amount of DNA from the strawberry and detergent mixture. Variables: The independent variable in this experiment is the strawberry mixture while the dependent
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QUIZ 02 ECO402 Quiz # 2 ECO402 (Microeconomics) Semester Spring 2008 Total Marks 15 Instructions: 1. 2. 3. 4. 5. This quiz covers Lesson 14-17. Last date for submission of quiz is 12/05/08. Upload your quiz with in due date and time. No quiz will be accepted after due date and time. In MCQs‚ write only one option in the answer sheet which you think is correct. More than one answer will be marked zero. 6. No quiz will be accepted through e-mail. 7. Please submit your solution
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Abstract The purpose of this experiment was to perform a liquid-liquid extraction method to extract the caffeine from the tea bags that were provided‚ and then recrystallize the caffeine. The solvents used in the experiment were an aqueous sodium carbonate and dichloromethane (DCM). Anhydrous calcium chloride pellets were used to dry the solution and emulsion layer and the DCM was then decanted. After washing the anhydrous calcium chloride pellets with more DCM‚ the solvent was evaporated‚ leaving
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Procedure 2: DNA Extraction from Cheek Cells Materials: Water‚ Clear Dish Soap‚ Table Salt‚ Isopropyl Alcohol (70%) or Ethanol‚ Food Coloring 1. To 200 Ml drinking water add two teaspoons of salt 2. Gargle the salt water for 1 minute. 3. Spit the gargled water into a beaker (or new cup). Now your cheek cells are suspended in the salt water. 4. Gently stir the salt water with one drop of soap (try to avoid air bubbles) 5. In a separate beaker (or cup)‚ mix 20 ml isopropyl alcohol and 1-3 drops
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DATE: September 16‚ 2012 TO: Patricia Bennett‚ Supervising Principal FROM: Connor Sims‚ Associate SUBJECT: Oil Drilling & Gas Extraction Industry in the US Analysis (21111) This report presents information regarding the industry‚ the primary operator of oil and gas field properties. The industry fuels its key buyers‚ the Natural Gas Distribution (22121) and the Petroleum Refining (32411) industries‚ with crude oil and natural gas. The industry continuously battles
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We wanted to extract‚ see and analyze DNA from a single strawberry( 12.11 g). The long‚ thick fibers of DNA store the information for the functioning of the chemistry of life. DNA is present in every cell of plants and animals. The DNA found in strawberry cells can be extracted using common‚ everyday materials. To start the procedure we needed the following supplies; one strawberry‚ one plastic cup‚ 10 ml of Cell Lysis Buffer (10% detergent‚ 1% NaCl)‚ one plastic zip-lock bag‚ one coffee filter‚
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Micro-extraction of urine and/or stomach contents and analysis by TLC Abstract Thin Layer Chromatography (TLC) is one of the oldest chromatographic techniques used for the identification of compounds and for determination of the presence of trace impurities. The aim of the experiment was to determine two unknown samples (X and Y) by comparing results to the known standard drugs‚ of which there was five‚ using a specific TLC method. The same technique was used for the determination of
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Extraction of DNA from Calf or Hog Thymus/Isolation of Yeast RNA I. Abstract Nucleic acids may be divided into two groups RNA and DNA. DNA contains almost all the genetic information while RNA serves as the bridge between the DNA and proteins. Study of both DNA and RNA initially involves proper extraction/isolation. The storehouse of eukaryotic DNA is the nucleus (and in the mitochondria)‚ so experimentally‚ DNA is extracted from tissues that have a high nuclear to cytoplasmic mass ratio‚ such
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